不同温度下应用比值控制实现连续流好氧颗粒污泥短程硝化梁东博

39420184ENVIＲONMENTALSCIENCEVol．39No．4Apr．20181121111*1．1001242．100045AGSDONH+4-NDO/NH+4-NＲ30、20、10℃Ｒ．30、20、10℃Ｒ0.50±0.05、0.35±0.030.20±0.02．．fluorescenceinsituhybridizationFISHAOBNOB．．X703.5A0250-3301201804-1713-07DOI10．13227/j．hjkx．2017080672017-08-092017-09-142015ZX07202-0131992～[e-mail]*[e-mail]ationinaContinuous-flowAerobicGranularSludgeＲeactoratDifferentTemperaturesThroughＲatioControlLIANGDong-bo1BIANWei1KANＲui-zhe2WANGWen-xiao1ZHAOQing1SUNYi-qi1LIJun1*1．NationalEngineeringLaboratoryofUrbanSewageAdvancedTreatmentandＲesourceUtilizationTechnologyCollegeofArchitectureandCivilEngineeringBeijingUniversityofTechnologyBeijing100124China2．CityPlanningTechnologyServiceCenterofBeijingBeijing100045ChinaAbstractMatureaerobicgranularsludgeAGSwasinoculatedinacontinuous-flowreactortotreatlowammoniasewageandthefeasibilityofachievingpartialnitritationinacontinuous-flowaerobicsludgesystemandthedemandforＲvaluetheratioofdissolvedoxygenandammonianitrogenwhenpartialnitritationisachievedatdifferenttemperatures3020and10℃wereinvestigated．ThecontrolstrategywasdesignedtomaintainaconstantratiobetweendissolvedoxygenDOandammonianitrogenNH+4-N．Theresultsrevealedthatstablenitritationinacontinuous-flowaerobicsludgereactorcouldbeachievedviaratiocontrolandthevalueofＲwere0.50±0.050.35±0.03and0.20±0.02atthetemperatures3020and10℃respectivelyfromwhichitcanbeconcludedthatstrongeroxygen-limitingconditionswererequiredwhenthetemperaturewaslower．TheexperimentoffluorescenceinsituhybridizationFISHrevealedthatammoniaoxidizingbacteriaAOBhaveacertainconcentrationandtherelativenumberofnitriteoxidizingbacteriaNOBgraduallyreducedthroughtheratiocontrolstrategy．Basedontheratiocontrolstrategyandthecharacteristicsofwastewaterqualityfullnitritationofhighammoniawastewatermaybeallowedhoweverforlowammoniawastewateronlypartialnitritationisrecommended．Keywordslowtemperaturepartialnitritationaerobicgranularsludgecontinuous-flowratiocontrol、、、、．1～3．、、4～6．AGS．AGSDOAGSDODO．78．AOBNOB39AGS．AGSSBＲSBＲ9～11．SBＲ．．1213、FA14、15、pH16．DO/TAN17．SBＲAGSAGS．11.110.8L1.4L．4cm．1.2SBＲAGS3.0mm60m·h－1MLSS3500mg·L－1MLVSS/MLSS=0.7．NH+4-NNH4Cl60mg·L－1CODCH3COONa300mg·L－1PKH2PO44mg·L－1．NaHCO31．2．3．4．5．6．7．8．pH9．DO10．1Fig．1SchematicdiagramoftheexperimentaldevicepH7.8～8.2．1L0.5mLFeCl3·6H2O1500mg·L－1、H3BO3150mg·L－1、CuSO4·5H2O50mg·L－1、KI150mg·L－1、MnCl2·4H2O110mg·L－1、CoCl2·6H2O150mg·L－1、Na2MoO4·2H2O60mg·L－1、ZnSO4·7H2O120mg·L－1．1.360mg·L－1．DONH+4-N．ＲＲ．Ｒ．A～H81．1.4Harremoes181．DO＜γO2/NH+4DNH+4/DO2NH+41DONH+4DONH+4DNH+4DO2γO2/NH+4．Bartrolí19．DONH+4-NAOB．DONH+4-N．DOHＲTNH+4-N．ＲDO/NH+4-NＲ417141Table1Operatingconditionsofthereactor/℃DO/mg·L－1NH+4-N/mg·L－1Ｒ/dA308.0±0.210.0±0.50.80±0.0510B307.5±0.215.0±0.50.50±0.0520C308.0±0.210.0±0.50.80±0.0520D307.5±0.215.0±0.50.50±0.0520E207.5±0.215.0±0.50.50±0.0520F207.0±0.220.0±0.50.35±0.0320G107.0±0.220.0±1.00.35±0.0330H105.0±0.225.0±1.00.20±0.02402Fig．2NitritationperformanceofthereactorDONH+4-NDONH+4-N．ＲDONH+4-N．DONH+4-NＲ2．1.5NH+4-NNO－2-NN-1--NO－3-NDO、TpHWTWMLSSMLVSS517139CODCOD．2NAＲ=NO－2-N/NO－2-N+NO－3-N2NO－3-N、NO－2-NNH+4-N．1.6FISHAOBNOB20．4%PFA4℃2h．4min50%、80%98%3min．46℃2h．250mL48℃15min．FV120010Imageplus-pro6.0．2Table2ProbesusedforFISHandthecorrespondinghybridizationconditions5'-3'NSO1225CGCCATTGTATTACGTGTGAAmmoniaoxidizingβ-ProteobacteriaNIT3CCTGTGCTCCATGCTCCGNitrobacterEUB338GCTGCCTCCCGTAGGAGTMostbacteriaEUB338ⅡGCAGCCACCCGTAGGTGTPlanctomycetalesEUB338ⅢGCTGCCACCCGTAGGTGTVerrucomicrobiles22.1SBＲAGS30℃HＲT8h．AGSAGSSBＲ．AGS5～7dNH+4-N10mg·L－1COD80%NAＲ90%．AGS．10d．Ｒ＜0.821．Bartrolí19Ｒ0.170.35．Ｒ0.8．NH+4-N、NO－3-NNO－2-N2．AＲ0.832.01mg·L－1NAＲ12.69%．BＲ0.55dNO－3-N6.37mg·L－1NH+4-N75%NAＲ80.32%．C、DA、B．Ｒ=0.8A、CＲ=0.5B、DAGS．ＲE30℃20℃Ｒ0.5．FＲ0.35．G10℃Ｒ0.35NAＲ59.95%．FＲ0.20．Ｒ．DO5～8mg·L－1B、D、F、H4NAＲ95%．DO．DONH+4-NC．DONH+4-N．DONH+4-N61714．2.2AOBNOB30℃A～D、20℃E～F、10℃G～H3．NH+4-N、NO－3-NNO－2-N2．B、D30℃Ｒ0.5E10℃Ｒ0.5FＲ0.35．10℃GＲ0.35HＲ0.20．．Bao22deKreuk23AGSAGS．AGS．AGS、24．AGSAOBNOBNOBAGSNOBNO－2-NNO－3-N．NH+4-NAOBAGSNH+4-NAOBAGSAGSNOBNOB．Ｒ．2G、HNAＲ59.95%AOBNOBAOBNOB．2.3FISHFISHC、D、F、H4AOBNOB．3．AOBAOBNOBNOBAOBNOB．3FISHFig．3ＲesultsofFISHanalysisCNO－3-N30mg·L－1AOB13.16%NOB2.53%DNO－3-N1.0mg·L－1AOB13.68%NOB2.14%FNO－3-N1.0mg·L－1AOB14.33%NOB2.05%HNO－3-N1.0mg·L－1AOB14.21%NOB1.69%．NOBNOBNO－3-N2．717139NOBNOB25．CD．AOBNOBAOBNOBNOB．2.4．100%57%．．．500～1000mg·L－195%25mg·L－110℃Ｒ0.20DO5mg·L－1．10℃Ｒ0.20DO5mg·L－1．．26NH+4-N50mg·L－1．5mg·L－130℃Ｒ0.5DO2.5mg·L－1．AGS27．NH+4-NNH+4-N．．20℃NH+4-N20mg·L－1DO7.0mg·L－110℃NH+4-N30mg·L－1DO6.0mg·L－1AGS．G．．31DO/NH+4-NNH+4-N．230、2010℃Ｒ0.5、0.350.20Ｒ．．3NOB．4．1TurkOMavinicDS．Maintainingnitritebuild-upinasystemacclimatedtofreeammoniaJ．WaterＲesearch198923111383-1388．2GuoJHPengYZWangSYetal．Long-termeffectofdissolvedoxygenonpartialnitrificationperformanceandmicrobialcommunitystructureJ．BioresourceTechnology2009100112796-2802．3TurkOMavinicDS．BenefitsofusingselectiveinhibitiontoremovenitrogenfromhighlynitrogenouswastesJ．EnvironmentalTechnologyLetters198781-12419-426．4LiuYYangSFXuHetal．BiosorptionkineticsofcadmiumⅡonaerobicgranularsludgeJ．Proce