餐厨垃圾堆肥发酵菌株筛选

2012－02－012012－03－312010220110161985－。*。[e-mail]**550025HMD6BacillusCohn1872、Z4BacillusCohn1872、X4Brevibacillusshida1996。D626.48U/mLZ46.67U/mLX44.05U/mLHM。D6∶Z4∶X4=1∶1∶1、12.00U/mL、1.83U/mL76.93U/mL。HMHM。Q939.96A1008－0457201202－0153－04。EM、HM、VT、。。HM15～20/kg。HMGBNY525－2002C/N。EM、HM、、、、HM。。11.11.1.1HMEM。1.1.21MRSPDALB。2123。3145。1.21.2.1628、50、55℃。312153～1562012JournalofMountainAgricultureandBiology4℃。1.2.2。DdD/dD/d。1.2.3OD520nm=0.61%4℃7000r/min。789DNS8。1.2.4D/d1987HM。1.2.53∶260%121℃20min。、、OD520nm=0.61%40℃2d1∶104℃7000r/min。1.2.3。10。HMHMHM。40%60%1/dHMHM。HM。22.1151、135、10753。446。D/d857D1～8、Z1～5X1～7。201。3D/dD/d1.50。D1～8、Z1～5X1～7。14。D2、D4、D6、Z2、Z3、Z4、X1、X4、X7。1、、Tab.1ScreeningresultsofstrainswithactivitiesofamylaselipaseorcellulaseDdD/dD/dD/d①D/d②D/d③U/mLD15.003.752.272.066.9D23.863.863.673.7839.43D34.143.603.564.0014.61D44.003.954.084.2324.64D53.223.003.103.005.11D64.504.004.034.4426.48D73.293.103.333.3310.80D83.403.833.773.5722.09Z12.202.202.272.155.42Z22.102.002.002.048.33Z32.802.752.802.696.67Z43.964.004.003.976.67Z52.552.003.002.716.59X16.007.005.504.063.43X24.504.755.337.672.57X35.004.503.334.000.38X47.006.679.508.504.05X54.005.004.254.251.24X66.507.003.634.443.19X74.676.356.006.675.22“D/d”“D/d①、D/d②、D/d③”D1－D8Z1－Z5X1－X73。2.29HM。21D6、X4HMD6、X4。Z3、Z4HMZ3X44512012Z3Z4。、、D6、Z4、X4。2Tab.2ResultsofantagonisticexperimentsD4D6Z2Z3Z4X4X7HMD4－－+++++++D6－－+－－+－Z2+++－－+－－++Z3+－－－－+－－Z4+－+－－－+－X4+－－+－－－－X7++－－+－－+HM+－++－－－+－6mm“+”7mm8mm“++”8mm“－”7mm。2.3。HM。3M3M1M1M3M3M1HM。M1M1HM1∶1HM。3HMHMM1HM。64.4%。NY525－20024HMC/NHMHMHM。M1HMHM。3U/mLTab.3ActivitydetectionofdifferentcompoundmicroorganismM1M2M3HMHM12.009.907.0524.7525.131.831.332.3315.5219.7776.9334.2631.7542.3769.95“M1”D6∶Z4∶X41∶1∶1“M2”D6∶Z4∶X41∶1∶2“M3”D6∶Z4∶X41∶1∶3。4HMHMTab.4DetectionresultsofcompostingproductswithHMorHM-mixedagent%%C/NHM41.085.1417.77HM36.066.5712.70NY525－2002≥30.0≥4.033.1。。。3.211－13。、。。350℃。D6、X4HMZ4。3———HMHM。55123.3。。。。。1.CB－7J.200947－1021.2.UHTJ..2008291230－233.3.J.2011277102－106.4.J.2006253261－268.5.J.200727454－57.6.J.20112058－60.7.M.3.2006.8QB/T1803.93S.9HorikoshiKNakaoMKuronoYetal.CellulasesofanalkalophilicBacillusstrainisolatedfromsoilJ.CanJMicriobiol198430774－779.10.D.2006.11.J.201020112817－2820.12.D.2011.13.J.200626107－110.ScreeningofKitchenWasteFermentationStrains*GUOQian-qianWUYong-jun*LUBiaoHANLi-zhenCollegeofLifeSciencesGuizhouUniversityGuiyangGuizhou550025ChinaAbstractTopromotethedegradationeffectofkitchenwastthreeeffectivestrainsD6Z4andX4ofincompostfermentationwerescreenedfromthechiefdomesticcommercialstrains．D6Z4andX4whichpro-ducesamylaselipaseandcellulasegavetheenzymeactivitiesof26．48U/mL6．67U/mLand4．05U/mLrespectively．NoobviousantagonismamongstrainsaswellasbetweenstrainsandHMwereinvestigated．WiththeproportionofD6∶Z4∶X4=1∶1∶1thebesteffecthadbeenobtainedwhichproduced12．00U/mLamylase1．83U/mLlipaseand76．93U/mLcellulase．ComparedwiththesinglestrainmultiplestrainHMgavethebettereffect．KeywordskitchenwasteAmylaseLipaseCellulase6512012