产酸相中氧化还原电位控制及其对葡萄糖厌氧发酵产物的影响

31Cord-RuwischRalf211(11,214036;21BiotechnologyDivisionofScience,MurdochUniversity,Perth,6150,Australia):(rumen),,,pH65h10h,Eh-550-300mV,,Eh,EhEh-200mV,,,40%Eh-100mV,pH315,,;,:;;;;:X703,S21614:A:1000-1166(2000)4-0020-04TheStrategyofRedoxPotentialControlandItsInfluenceonAnaerobicFermentationofGlucoseinAcidogenicReac2tor/ZHOUHong2bo1;Cord2RuwischRalf2;CHENJian1;RENHong2qiang1(11SchoolofBiotechnology,WuxiUniver2sityofLightIndustry,Wuxi214036,P.R.China;21BiotechnologyDivisionofScience,MurdochUniversity,Perth,6150,Australia)Abstract:Glucosewasfermentedinachemostatunderanaerobicconditioninoculatedbymixtureoftwokindsofsludgesandrumenfromsheep.Redoxpotentialwascontrolledbyaerationandstirring.Resultsshowedthatredoxpotentialstronglyaffectedproductsdistribution.UnderconditionsofpH6,HRT5hand10h,acetateandlactateweremajorproductsatEh-550-300mV;WithincreaseofEh,butyratedecreasedbutpropionateincreased;allproductsdecreaseddramaticallyexceptethanolwhenEh-200mVwhileelectronrecoverywasonly40%.YeastwasthedominantmicroorganismandethanolwasthemajorproductwhenEh-100mV,pH315HigherEhvalueswasfavourableforyeastgrowthandlowerEhfavoeurableforetha2nolformation.Keywords:acidogenicreactor;redoxpotential;glucose;anaerobic;fermentationproduct.HaesungJee[1]Eh-370-500mV,Eh-315-350mVFetzerhConrad[2],015%(Eh+100mV)Methanosarcinabarkeri32000:2000-07-06:2000-09-15,pH,,,PP,,,02ChinaBiogas2000,18(4),11111,013L,,35011pH4MNaOHHCl,PCLabview(2,3),()=(-)111;21;31;41;51;61;71Eh;81pH;91pH;1012MHCl;1112MNaOH;121;131;141;151;161;171;18,191Eh,,01101001,Eh,,Eh,5mV5mV,,Eh,,,Eh,112(gL-1):21,1,K2HPO4012,KH2PO4014,NH4Cl3,KCl015,CaCl2012,MgSO47H2O011,NaCl1,Na2SO40.51mlL-1[3]113,,255200ml20ml80%N220%CO210min-100mV,,10,114(YSI2700SE2LECT,JohnMorrisScientificPtyLimited)()OLYMPUS578nm105115,C2HC2C,:()=C2CC2H(%)=P10012ChinaBiogas2000,18(4)22112:pH100,Eh-1,,,,Eh(2),,Eh5mV(3)3:pH100,Eh-1212pH,pH6,pH6HRT10hEh(4),,Eh-550-300mV,Eh,EhEh-300mVEh-200mVEh-200mV,,,40%4pH6,10h,Eh(-550-300mV),Eh,Eh,,[4]213EhpHpH,pHpH315,,pH6,Eh-100mV,,,,EhpH,pH,pH3151,,pH210,95%,22ChinaBiogas2000,18(4)1pHEhpHEhPmVODP578nmPmmolL-1P%P%315-80014,12212711316718941068143-50018,103133162116149916691121600197311513113512851259182125001914812510010813551239142128001933711218010510651641102121000195311111801051060104312,(1),,40%70%pH,pH6,,(23d)HRT5h,10h,,,,Eh,,Eh,,,Eh-800mV,,Eh0mV,,,[5,6]3(1),(2)Eh-550-330mV,,Eh,EhEh-220mV,,(3),,,,Eh-80+100mV,,[1]HaeSungJee,NaomichiNishio,ShiroNagai1Influenceofre2doxpotentialonbiomethanationofH2andCO2byMetha2nobacteriumThermoautotrophicuminEh2statbatchcultures[J].JournalofGenernalAppliedandMicrobiology,1987,33:401-4081[2]FetzerSilke,ConradRalf.Effectofredoxpotentialonmetha2nogenesisbyMethanosarcinaBarkeri[J].Arch.Microbiol,1993,160:108-113.[3]ZehnderAJB.,HuserBA,etal.Characterizationofanac2etate2decarboxylating,non2oxidizingmethanebacteria[J],Arch.Microbiol,1980,124(1):1-11.[4]InancB,MatsuiS,IdeS.Propionicacidaccumulationandcontrollingfactorsinanaerobictreatmentofcarbohydrate:effectsofH2andpH[J].Wat.Sci.Tech.,1996,34(5-6):317-325.[5]ChungIS,LeeYY.EffectofoxygenandredoxpotentialonD2xyloseDeok2KunOh,fermentationbynongrowingcellsofPachysolentannophilus[J].EnzymeMicrobiol.Tech.,1986,8:503-507.[6]OhDoek2Kun,KimSang2Yong,Kimjung2Hoe.Increaseofxylitolproductionratebycontrollingredoxpotentialincan2didaparapsilosis[J].Biotech.Bioeng.,1998,58(4):440444132ChinaBiogas2000,18(4)