常温限氧条件下SBR反应器中的部分亚硝化研究田智勇

SBR,,,,,((),　100022):(14.1～24.2℃)(NH+4-N30～100mgL),(DO0.3～0.4mgL)SBRANAMMOX.ANAMMOXNH+4NO-2=11.31,.,DO,,NH+4-NNO-2-N.57%,HCO-3NH+4pH.pH,(OUR),DO(DO1.0mgL),.30～100mgL4.,(NO-2NH+4),ANAMMOX.:;;;;:X703.1　:A　:0250-3301(2008)04-0931-06:2007-04-04;:2007-06-21:(863)(2003AA601010):(1978～),,,,E-mail:[e-mail]SBRUnderNormalTemperatureandLimitedOxygenConditionTIANZhi-yong,LIDong,CAOXiang-sheng,ZHANGShu-de,YANGHong,ZHANGJie(KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing100022,China)Abstract:PartialnitrificationwasresearchedinaSBRreactorunderthelimitedoxygencondition(DO0.3-0.4mgL)andthenormaltemperatureof14.1-24.2℃toproducethecorrectinfluentforANAMMOXprocessfromsecondarywastewater(NH+4-N30-100mgL).TheinfluentfortheANAMMOXprocessmustbecomposedofNH+4andNO-2inaratioof11.31andthereforeonlyapartialnitrificationofammoniumintonitritewasrequired.ThecontrolofalkalinityandtheindicationofmonitoringprofileofDOallowedtoachievethispartialnitrificationwithafinaleffluentonlycomposedofNH+4-NandNO-2-Nattherightstoichiometricratio.TheequalconsumptionofHCO-3NH+4insecondarywastewaterresultedinanaturalpHdecreasewhenapproximately57%ofNH+4-Nwasoxidized.WhenthepHdroppedtoacertainlevel,theloweringofthemetabolicrateoftheammoniumoxidizerwouldleadtorapiddeclineoftheoxygenuptakerate(OUR)inreactor,andthejumpcharacteristicpoint(itwasdistinguishedbythejumpover1.0mgLofDOvalue)intheDOprofilewouldemerge,whichsignalledtheend-pointofthepartialnitrifying.Theoptimumalkalinityofpartialnitrificationwasstudiedunder4concentrationsintherangeofNH+4-N30-100mgLofsecondarywastewater.Theresultindicatedthatthebicarbonateammoniumratiowasthekeyfactorinfluencingthenitrificationratio(NO-2NH+4),anditwasabsolutelypossibletosuccessfullyachievepartialnitrificationthatprovidedrightsubstrateforANAMMOXreactorthroughthecontrolofthealkalinity.Keywords:advancedtreatment;limitedoxygencondition;partialnitrification;ANAMMOX;alkalinity　　(anaerobicammoniumoxidation,ANAMMOX)[1].,,、CO2、,[2].,,ANAMMOX[3][(1)],NH+4-NNO-2-N11.31.NH+4+1.31NO-2+0.066HCO-2+0.13H+1.02N2+0.26NO-3+0.066CH2O0.5N0.15+2.03H2O(1)　　,ANAMMOX,、、.,,NH+4-NNO-2-N.,.,29420084　　　　　　ENVIRONMENTALSCIENCEVol.29,No.4Apr.,2008DOI:10.13227/j.hjkx.2008.04.009ANAMMOX.,NH+4-NNO-2-NANAMMOX.,pH7.9～8.2、30～35℃、DO(1mgL)[4～6],(KS=0.3mgL)(KS=1.1mgL)[7],.,DO、pH,,.,,NH+4-N800～1000mgL,37℃、SRT2d,(SHARON),[5,8～10].,.,(14.1～24.2℃),AO(),(DO0.3～0.4mgL)SBR(NH+4-N30～100mgL),ANAMMOX.1　1.1　SBR,6.6L,5L,1.42mm,5%,SBR,,10mm.,,,.(A=1264mm2,200rmin),,.SBRSV30、SVI、MLSS、NH+4-N、NO-2-N、NO-3-N,DO、ORP、pH.1.2　AO()(COD50～60mgL,1.pH;2.pH;3.ORP;4.ORP;5.DO;6.DO;7.;8.;9.;10.;11.12.1　Fig.1　Experimentalapparatus　NH+4-N80～110mgL,NO-2-N1mgL,NO-3-N1mgL,TP0.18～0.74mgL,14.1～24.2℃,pH7.65～7.79).,.1.3　,,MLSS4.85gL,1L.,0.10～0.15Lmin,DO0.10～0.20mgL.pH、ORPDO,DO,,1.5h.1,MLSS1000mgL,MLVSS820mgL,SV30SVI5%50mLg,98%,90%,95%,.,.,.,,,2～3,.,,,,DO0.03～0.60mgL,6.,pH、ORPDO,3,1.5h,3932　　　　　　29.ANAMMOX,(14.1～24.2℃)(DO0.3～0.4mgL)4,,1.1　mg·L-1Table1　Influentconcentrationofammoniumandalkalinityinthepartialnitrificationtestmg·L-11)191.8～102.6(97.2)2)218324445548631356.3～58.8(57.6)228278332383434273.7～81.5(77.3)149251379460435.6～36.7(36.6)2382642893151)CaCO3,pH3.7;2)2　1SBRFig.2　ProfilesofoperatingvariablesandnitrogencompoundsofnitrificationinaSBRcircle1.4　NH+4-N;NO-2-NN-(1-)-;NO-3-N;DOWTWinoLabStirrOxG;pHOAKLONWaterproofpHTestr10BNCpH;ORPOAKLONWaterproofORPTestr10ORP;、MLSS、MLVSS、SV30SVI[11,12].25mL,30min,15mL.,.2　2.1　,,95%,,0.86kg(kg·d),0.70kg(m3·d),[13～15].[16](35±1)℃、(860～3500mgL)1.39～3.01kg(kg·d),、DO.21(DO、pHORP).,,pHORP,,,,DO9334:SBR.,,,DO,;,ORPDO1(2“★”);,CO2,pH,“pH”[17];,pHORP,pHDO,ORP2,(2“◆”).,6,,.2.2　SBR4,1.(0.028～0.032m3h).,0.3～0.4mgL.,,HCO-3NH+4pH.《》(8),pH5.8～8.5,.[18,19],pH6.5,pHNH3NH+4,,.,pH,,(OUR),DO(DO1.0mgL),.1,DO、ORP、pH334.DO,1,.、pH;.,pH3　Fig.3　Profilesofoperatingvariablesofpartialnitrification4　Fig.4　Profilesofnitrogencompoundsconcentrationofpartialnitrification934　　　　　　29“pH”,;ORP,(3).(DO1.0mgL)[NO-2(NO-2+NO-3)]97%,3%,.3pH(4).2.3　,pH,.pH,(OUR),DO(DO=1.0mgL),.(NO-2NH+4).4(NO-2NH+4)5.5,4(R20.9992、0.99990.9988),.4,,(5,1.97).4,,,.5　Fig.5　Relationshipbetweennitrificationratioandalkalinity　5,4(NO-2NH+4=1.31).4c(NH+4-N),,(6).6,,R2=0.9992.k=3.8242,(-107.84)3.8242,,DO1.0mgL,,ANAMMOX(NO-2NH+4=1.31).6　Fig.6　Relationshipbetweenthealkalinitydemandedinpartialnitrificationandtheinfluentammoniumconcentration　2.4　(1000mL)1L,40g()1,,(681-)h,25～35℃,10%NaOH,.,(N2),11,,pH,ANAMMOX.,SBRANAMMOX.,ANAMMOX,,,ANAMMOX.3　　　(1)(14.1～24.2℃),AO,9354:SBR,SBR.(2),.(3),.(4),NO-2NH+4,DO1.0mgL,(-107.84)3.8242,SBRANAMMOXNO-2NH+4=1.31.:[1]MulderA,vandeGraafAA,RobertsonLA,etal.AnaerobicAmmoniumOxidationDiscoveredinaDenitrifyingFluidizedBedReactor[J].FEMSMicrobiologyEcology,16(3):177-183.[2]VandeGraafAA,deBruijnP,RobertsonLA,etal.MetabolicPathwayofAnaerobicAmmoniumOxidationontheBasisof15NStudiesinaFluidizedBedReactor[J].Microbiology,1997,143:2415-2421.[3]StrousM,HeijnenJJ,KuenenJG,etal.Thesequencingbatchreactorasap