潮间带产絮凝剂放线菌的筛选及其培养条件的优化耿强

　　2011,Vol.28No.7Chemistry&Bioengineering70　　　:2011-04-27:耿强(1987-),男,山东临沂人,硕士研究生,研究方向:重质油加工和环境微生物应用研究;通讯作者:刘其友,讲师。E-mail:[e-mail]。doi:10.3969/j.issn.1672-5425.2011.07.018　1,2,　3,1(1.(),266555;2.,300073;3.,100083)　:从唐岛湾潮间带泥沙中筛选出放线菌18株,进行含油废水实验复筛后得到较高絮凝活性的菌株Y-3,初步鉴定为链霉菌属。采用单因素实验和正交实验对Y-3菌株进行发酵条件的优化。结果表明,Y-3菌株最适培养基为:乙醇为碳源,酵母膏为氮源,低盐度;最适培养条件为:培养温度30℃,初始pH值7.0,乙醇用量35mL·(L海水)-1,摇床转速130r·min-1。在最优条件下,絮凝剂对高岭土的絮凝率达到92.76%,对含油废水也具有较好处理效果。:潮间带;放线菌;生物絮凝剂;含油废水:X703　　　　　:A:1672-5425(2011)07-0070-04　　、、,、、、、、[1]。,,,。,Y-3,。1　1.1　。:20g,NaCl0.5g,KNO31g,K2HPO4·3H2O0.5g,MgSO4·7H2O0.5g,FeSO4·7H2O0.01g,20g,KCrO40.1g,1000mL,pH7.4～7.6。:20g,NaCl0.5g,KNO31g,K2HPO4·3H2O0.5g,MgSO4·7H2O0.5g,FeSO4·7H2O0.01g,KCrO40.1g,1000mL,pH7.4～7.6。:。1.2　1.2.1　,。NikonTS100、,[2]。1.2.2　,pH、、。,、,4pH、、、[3],1。1Tab.1FactorsandlevelsoforthogonalexperimentA.pHB.℃C.mL·(L)-1D.r·min-116.5281513027.0302513537.532351401.2.3　　:/20117　71　　　500mL300mL,,,(200r·min-1)2min,(60r·min-1)6min,10min。50mL,、COD。COD、、、[4]。1.2.4　[5],。250mL150mL4g·L-1、3mL,,。(200r·min-1)1min,(60r·min-1)3min,5min。,721OD550。。:　　=A-BA×100%:A;B。2　2.1　2.1.1　3,,,。18,2。1。2/%Tab.2Flocculationactivitydeterminationvaluebypreliminaryscreening/%1#17.217#52.4013#36.902#-4.118#0.9314#54.843#85.049#-7.9815#62.444#80.1610#81.7416#49.265#12.3311#5.7817#36.016#23.4812#78.5318#41.3621,,,1　Fig.1　FlocculationeffectdistributionofActinomycetes。(80%)3,16.67%,。2.1.2　3#、4#、10#,3。3/%Tab.3Flocculationeffectonoilywastewaterbysecondscreening/%COD3#59.1214.7619.444#57.2911.8813.2210#53.1812.4618.213,3#,,Y-3,。2.1.3　Y-3,2。2　Y-3Fig.2　IndividualandcolonialmorphologyofstrainY-32,,,,,,,,。　　:/2011772　　　,、,,、,[6]。,Y-3。2.2　2.2.1　(4)4/%Tab.4Theoptimizationofthemediumcomposition/%NaClg·L-181.12KNO385.040.585.0486.8887.431.560.8585.0486.773.038.074,、,。,,。,,,。,[7]。,。2.2.2　pH(3)3　pHFig.3　InfluenceofinitialpHvalueonflocculatingeffect　　3,,pH;(pH=6.0～7.5),;,,[4]。2.2.3　(4)4　Fig.4　Influenceofculturaltemperatureonflocculatingeffect　　4a,pH,pH,30℃pH。pH,NH+4,,pH。,,,,[8],,,pH。4b、c,30℃,,,[9],,,,0。2.2.4　(5)5　Fig.5　Influenceofrotationspeedonflocculatingeffect5,140r·min-1,Y-3。2.3　(5)5,　:/20117　73　　　5Tab.5ResultsandanalysisoforthogonalexperimentforculturalconditionoptimizationABCD/%1111179.262122291.433133386.534212385.725223192.766231284.417313282.478321387.059332181.37k185.7482.4883.5784.46k287.6390.4186.1786.10k383.6384.1087.2586.43R4.007.933.681.97:pH,。,A2B2C3D1,30℃、pH7.0、35mL·(L)-1、130r·min-1,,Y-392.76%。2.4　Y-3。,66.4%,COD19.23%,23.64%,。3　(1)Y-3,:,,;30℃,pH7.0,35mL·(L)-1,130r·min-1。,92.76%。(2)Y-3,,66.4%、COD19.23%、23.64%,。:[1]　,,.———[J].,2004,35(1):52-55.[2]　,,,.19G-317[J].,2009,35(3):243-248.[3]　,.[M].:,2008:110-115.[4]　.()[M].:,2002.[5]　.[D].:,2003.[6]　.[M].:,1975:13-15.[7]　ZhangYubin,LinPeng,WeiXiaoyong,etal.Effectofsalinityonmicrobialdensitiesofsoilinthedilutionplatetechniqueappliedinmangroveareas[J].ActaEcologicalSinica,2008,28(3):1287-1295.[8]　,.[J].,2001,28(1):10-13.[9]　,,.[J].,2005,6(4):243-248.ScreeningandCulturalConditionOptimizationofBioflocculant-producingActinomycetesfromIntertidalZoneGENGQiang1,FENGSheng-ke2,ZHANGChao3,LIUQi-you1(1.CollegeofChemistry&ChemicalEngineering,ChinaUniversityofPetroleum,Qingdao266555,China;2.CollegeofChemicalEngineeringandTechnology,TianjinUniversity,Tianjin300073,China;3.ResearchInstituteofPetroleumProcessing,SINOPECCORP,Beijing100083,China)Abstract:Inthissudy,18strainsofActinomyceteswerescreenedfromintertidalzonesedimentofTangIs-landBay.Afterrescreeningontheoilywastewater,astrainnamedY-3withhighflocculantingactivitywasob-tainedandidentifiedasStreptomyces.Throughsinglefactorexperimentandorthogonalexperiment,theopti-mummediumcompositionwasobtainedasfollows:ethanolascarbonsource,yeastasnitrogensource,lowsa-linity.Theoptimumculturalconditionswereobtainedasfollows:culturaltemperatureof30℃,initialpHval-ueof7.0,ethanolamountof35mL·(Lseawater)-1,rotationspeedof130r·min-1.Thebioflocculanthadgoodeffectonoilywastewaterwithflocculatingrateof92.76%againstkaolinclaysuspensionundertheopti-mumconditions.Keywords:intertidalzone;Actinomycetes;bioflocculant;oilywastewater