臭氧预氧化对藻细胞完整性的影响及可同化有机碳的形成规律

13((),0092):,、。:,Aoc,,OAC。,,,OAC;,,,10M,Aoc。,AOC,,,Aoc。:;;;,(),。【`l,、2[],,131,。,,,,4IJ。,,(Intracellularorganiematter,IoM)l5]。(Extraceuularogranicmatter,EOM),,6[]。(Assimilableoaniceahton,AoC),。、nderkooijAOC。,AOC;,Aoc50100gL/,1317,8]。(Toc)(0.1%9.0%),,:(、、),(、、),、。“,,,。,AOC,,:;、。1.1.1(:HB905)。BG119[],,,28OC,200l0ux,12/12。,9[,’0],,14.0x10“/mL,。1.1.2O.ZmolNaZHPO;NaHZPO;,(NaZHPO4:NaHZPO4=39%:61%)(0.2M)。,10mM,pH=7.0.1,。,TOC(MulitNC/3100,),(1OmM)ToC=2.omg。1.1.3,,ToGcZ,,160mmin。,1L,.4ooC(),,。132030min,,,258nm,35,,。1.21..21(AOC),(FCM),AOC(l.ox07=lAoe)[。Aoe:(l)ZomL,0.22m(Millipore),700el。(2),。L20ml;(3):Evian(),5xlm/L;(4)30“C,,AOC。1.2.2:500,SYT0x(0.1M),30Oc810,。:cIPoEs(OtPima5300),0.22(Millipore),pH2.012[】TOCDOC:,MulitNC/3100,,:(IC)(TC),。TOC,;DOC,.022(Millipore)。1.2.3【13]。22.1t1(a),。1(b)、(c)(d)lmsL/、Zm3mg,smin,,,132030min,,,258nm,35,,。1.21..21(AOC),(FCM),AOC(l.ox07=lAoe)[。Aoe:(l)ZomL,0.22m(Millipore),700el。(2),。L20ml;(3):Evian(),5xlm/L;(4)30“C,,AOC。1.2.2:500,SYT0x(0.1M),30Oc810,。:cIPoEs(OtPima5300),0.22(Millipore),pH2.012[】TOCDOC:,MulitNC/3100,,:(IC)(TC),。TOC,;DOC,.022(Millipore)。1.2.3【13]。22.1t1(a),。1(b)、(c)(d)lmsL/、Zm3mg,smin,,,13:Toc.20Zmg;smin。2.250omg/L,(CO3)(Hco3);,.OH。2,,Zminsmin,O3+HCO303。,,smin,+HCo3030.3730.389,,。,03+HC0303Zmin,DOC24.0%29.9%,o3,。,,HC03,,.OH。2DOCCC(s)))%%%mg/LLLmg/LLLCCControlll0SSS75.6660.314441.04440003+HCO3---12055511.3330.379991.2999333330055510.9990.373331.3111OOO3331205551.3330,386661.355533333005550.5550.389991.2888:Toc=2.0.2mg;0.smM2.3tAOC2(a),AOC155,lmgL/3mgL/,AOC188469、389335。:(oIM),AOC。,AOC,EOMOIM,,AOC;,OIM13。smin,lmg~3mg,Aoc304g/L、312363L/,AOC,,。2(b)Aoc,。,03,AOc。1mg,.02mgL,Aoc280g。Zmg3mg,,.0smg,AOCZoogP/L146g/L。Hammes,,AOC!’5】。:l.oxlo,cel/ml:Toe2.o.lmgzL;pH6.90::200.soC.a()AOC(b)AOC2AOC2.4AOC3,,AOC。,155AOC225g;0.smMHcO3,AOc46g,80%。smin,03Aoc312,0.smMHeo3Aoe239.5g,l.6mMHCo3Aoe225g。,.OH,.OH,,。,AOC,13,AOC,AOC。[03]。=2.000.05mg/L;:1.ox10,eellzml:Toe2.0o.lmg/L;pH=6.90;:200.5“C.3Aoc2.5AOC,,AOC。,3,,AOC,480520,,,。3mg/L(CaCO3)UVZs46.2562.144.64}0.1391480520l0011Up7`lù7J4,,AOC,0.48mgmin,AOC246。SUVA3.0,,,AOC。,AoC,0.25mgmi,lmgL/2mgL/,AOC293L/13288.5。,,OIMAOC;AOC;,Aoc。,2mgL/,AOC,,,,IOM,AOC。[03]。2.000.05mg;:1.ox10,cellm/l;pH=7.50:4200.5“C;AOCAoc,,AOC。,。,3,、,Aoc。,2.om,smin。13[03]。2.000.05mg/L;:1.ox10,cel/m;smin;pH=7.80.02::200.5“C.5AOC5,,AOC480520。,AOC468,.64%AOC,AOC;,AOc427gL/,,,AOC。、Aoc220g、7448,AOC。,AOC。lmgL/、,,AOC28gL/、17978L/;Zmg,AOC145、342g222。,AOC,,,AOC。3(l);,,DOC,,,。,HC03,,。13(2),AOC,,AOC。,,,AOC。(3),AOC,,AOC,AOC。(4),AOC,,,AOC。[1]BernhardtH,ClasenJ.InvestigationsintotheMechan1smsof1994,5(43):222232.Sma11A1ga1Ce115.WaterF1OCCU1at1OnSupp1yRes,[2]Edzwa1dJ.A1gae,bub1es,coagu1ants,flotation.Water,1993,10(27):6781.andd15501V6dair[3]ParentL,TwissM.R.Influencesofnaturaldissolvedorganicmatterontheinteractionofaluminumwiththem1croaIgaChlorelIa:Atestofthefreeiondeloftracemetaltox1c1tv.Env1ronSc1.Technol,1996,5(30):17131720.of[4]P1umerJ,Edzwa1dJ.EffectsandoZoneona1ga1ce11ProPertiesWaterSupply,2002,6(51)andremOVach1or1nea1gaebycoagu1at1on.:307318.[5]Freder1kHaes,SeBastienMevlanElisabeth.Formationofassimilableorganiecarbon(AOC)andspecifienaturalorganicmatter(NOM)fract1onsdur1ngozonationofphvtoplankton.2007(41):14471451.[6]HendersonRitaK.Theimpactofdifferingcellandalgogenicorganicmatter(AOM)characteristicsonthecoagulationandflotationofa1gae.2010,44:36173624.[7]VanderKooijD.AssimilabIeorganiccarbonasanindicatorof9813bacterialregrowth.1992,2(84):5765.Journa!lnAer1canWaterWorksAssoeiation[8]Srinivasans,Harrinondr1nk1ngwaterd1stribution2007,10(41):21272138.G.W.Biostabi1ityanalysisforsyst5.WaterResearch,9[〕..:,2009:2662.【,0]..,2007(661):5[Ha.esF,Eg11Tomas.Newmethodfo:assimilableorganiccarbondeterminationusingflo--wcytometrieenumerationandanaturaImierobiaIeonsortiumasinoculum.EnvironmentalScience&Technology,2005,39(9):32893294.〔12」.:,2002:409412.[13]BaderH,Hoign`J.Determinationofozoneinwaterbvtheindimethod.WaterRes.,1981,15(4):,456.[14]JenJengChen,HsuanHs1enYeh.PermanganateOnaIgaeremoval.Water[15]Freder1kHa,es,Elandk1net1eeva!uat1onof1sabethRes,Sa1hiThemechan1smsofPotass1um2005,39:4420-28.0!iverKoster.Mechanisticorganicdisinfectionassimilableorganicearbon(AOC)formationduringdrinkingwater.WaterResearch,2006(40):22752286.byProductandtheozonat1onof99