处理高氨氮废水亚硝化细菌培养实验研究郑彭生

、A/O、SBR、ANAMMOX、CANON、OLAND[1-2]。NH4+-N→NO2--N4NO2--NNO3--N[3-5]。[6-8]。。11.1。(NH4)2SO42g、K2HPO40.75g、NaH2PO40.25g、MgSO4·7H2O0.03g、MnSO4·4H2O0.03gNa2CO3pH8.1～8.2121℃20min。1.2120L、、、、DOpH。1.35g4500r/min150mL30℃、120r/min15mL。61Fig.1SchematicdiagramofdirectionalculturedevicespH311201、pH、。0.75μm×0.3μmT=(28±1)℃、pH=(7.6±1)、ρ(DO)=(1.2±0.2)mg/L2.4×109CFU/mL21.8mg/(L·h)≥96.0%。X703A1000-3770(2018)[e-mail]:10.16796/j.cnki.1000-3770.2018.02.01444220182Vol.44No.2Feb.,201860NH4+-NNO2--N30℃。。pH、、DONH4+-NT=(28±1)℃pH=(7.6±1)ρ(DO)=(1.2±0.2)mg/L1d1NH4+-N250mg/L550mg/L。NH4+-NNO2--NN-(1)-NO3--NMPN。22.14、525510dρ(NH4+-N)=0NO2--N93%。2.20.75μm×0.3μm。2.32.3.1pH、pHpHρ(NH4+-N)=200mg/L、T=(30±1)℃、ρ(DO)=(1±0.2)mg/LpH3。pH≤8pHNH4+-N、NO3--NNO2--N、NH4+-N、NO2--NpH≥8.5pHNH4+-NNO2--NpHNO2--NpH≥8.5pHNH4+-N。2.3.24ρ(NH4+-N)=200mg/L、ρ(DO)=(1±0.2)mg/L、pH=8。T=25～40℃NO2--NNO2--N。25℃≤T≤30℃NO2--N87.4%T=25℃96.3%T=30℃NH4+-NNH4+-NT=30℃98.2%。30℃≤T≤40℃NO2--N。2.3.3DODO53pHFig.3InfluenceofpHonnitrosationeffect7.07.58.08.59.0050100150200250pH
/(mg
L)020406080100 /%2NH4+-NNO2--NFig.2ChangesofNH4+-NandNO2--Ninselectivesubculture024681012050100150200250300350400
/(mg
L)d4Fig.4Influenceofwatertemperatureonnitrosationeffect25303540050100150200250

/(mgL)020406080100  /%610.51.01.52.02.5050100150200250
(DO)/(mg
L)
/(mg
L)020406080100 /%5Fig.5Influenceofdissolvedoxygenonnitrosationeffect05101520250100200300400500600
d
/(mgL)020406080100 /%6Fig.6Nitrosationeffectinacclimatizationofhighammonia-nitrogenwastewaterρ(NH4+-N)=200mg/L、T=(30±1)℃、pH=8。NO2--NNO3--N。DODONH4+-N。ρ(DO)=0.5～2.5mg/LDONH4+-N。ρ(DO)=0.5～1mg/LNO2--N≥96%NO2--N。NO2--NDODONO2--NNO2--N。2.46NH4+-N22dNH4+-N≥94.5%21.8mg/(L·h)。NH4+-NNO2--N2.4×109CFU/mL≥96.0%。ρ(NH4+-N)≥400mg/LNH4+-NpH。315NO2--N510NH4+-NNO2--N93%0.75μm×0.3μm。2ρ(NH4+-N)=200mg/LpH=8、T=30℃、ρ(DO)=1mg/L。3NH4+-N22dNH4+-N≥94.5%21.8mg/(L·h)2.4×109CFU/mL≥96.0%。[1],,,.N2O[J].,2016,36(4):1260-1265.[2],,.、[J].,2016,67(10):4040-4046.[3],,,.CANON[J].,2013,39(9):15-19.[4],,,.PN-ANAMMOXPCB[J].,2015,36(7):2591-2596.[5]JIYEOLIm,JINYOUNGJung,HYOKWANBae,etal.CorrelationbetweennitriteaccumulationandtheconcentrationofAOBinanitritationreactor[J].EnvironmentalEarthSciences,2014,72:289-297.[6],,,.[J].,2016,36(4):1027~1032.[7]NICOLAPaccanelli,ARONNETeli,DAVIDEScaglione,etal.Comparisonbasedonenvironmentaleffectsofnitrogenmanag-ementtechniquesinamanuredigestatecasestudy[J].Enviro-nmentalTechnology,2015,36(24):3176-3185.[8]MOOMENSoliman,AHMEDEldyasti.DevelopmentofpartialnitrificationasafirststepofnitriteshuntprocessinaSequentialBatchReactor(SBR)usingAmmoniumOxidizingBacteria(AOB)controlledbymixingregime[J].BioresourceTechnology,2016,221:85-95.8744262ExperimentalStudyonNitrosobacteriaCultureforHighAmmonia-nitrogenWastewaterTreatmentZHENGPengsheng,WUXueqian,ZHOURulu,GUOZhongquan（CCTEGHangzhouEnvironmentalResearchInstitute,Hangzhou311201,China）Abstract:Toculturenitrosobacteriafortheshortcutnitrificationofhighammonia-nitrogenwastewater,theenrichmentprocessandinfluencingfactorsofnitrosobacteriawereresearchedwithselectivesubcultureandsequencingdirectionalculture.Theinfluencesofculturingconditionsonnitrosationeffectswereanalyzed,suchasthewatertemperature,pHanddissolvedoxygenconcentration.Theresultsshowedthat,theenrichednitrosobacteriabelongedtonitrosomonaswiththeshort-rodshapeof0.75μm×0.3μm.Undertheconditionsofwatertemperature,pHandρ(DO)was(28±1)℃,(7.6±1)and(1.2±0.2)mg/Lrespectively,withtheextensionofculturetimeandtheimprovementofinitialconcentrationofammonianitrogen,thenitrosobacteriagraduallyacclimatizedtothewastewaterwithhighammonianitrogen.Thenitrosobacteriaconcentrationreached2.4×109CFU/mL,theammoniaoxidationratereached21.8mg/(L·h)andtheNitrite-Ncumulativepercentagewasabove96.0%.Keywords：nitrosobacteria;shortcutnitrification;ammonianitrogen;enrichmentcultureComparisononEffectivenessofRotatingBiologicalContactorandRotatingBiologicalCageUsedforMustardTuberWastewaterTreatmentWANGJianhui1,CHENYoupeng2,GUOJingsong1,2,DONGYang2(1.FacultyofUrbanConstructionandEnvironmentalEngineering,ChongqingUniversity,400045;2.ChongqingInstituteofGreenandIntelligentTechnology,ChineseAcademyofSciences,400714:Chongqing,China)Abstract:Inordertocomparetheeffectivenessoftworotatingbiofilmreactorsforsaltyindustrialwastewatertreatment,thewastewaterfrommustardtuberfactorywasusedasprocessobject.Thefeaturesandpollutantremovalefficiencyoftworeactorsatstartingphaseandoperationphaseweremeasuredandcompared.WhentheremovalratesoforganicmatterandNH3-Nwerebothmorethan80%,andthethicknessofbiofilmwasmorethan2mm,itwasthemetricsofstartingphasesuccess.Theresultsshowedthat,therewasnosignificantdifferenceofbiofilmculturingratebetweenthetworeactorsatthestartingphase(35dand31d,respectively),buttherotatingbiologicalcagehadhigherremovalefficiencyoforganicmatterandNH3-Nun