处理乙硫醇废气生物滤池中微生物的初步鉴定

:02532468(2004)02033305:X7017:A朱国营,刘俊新*(,100085):,.,,.,,.:;;;IdentificationofmicroorganismsinanEMdegradingbiofilterZHUGuoying,LIUJunxin(ResearchCenterforEcoEnvironmentalSciences,ChineseAcademyofSciences,Beijing100085)Abstract:Biofilterisoneofthebiotechnologiesforwastegastreatment.Bacteriaandfungiarethetwodominantmicroorganismgroupsinbiofilters.Thedegradingabilitytohydrophobicorganiccompoundandadaptabilitytoacidanddryconditionsoffungiarehigherthanthoseofbacteria.Inthisexperimentalresearch,abiofilterwasusedtotreatawastegaswithethylmercaptan(EM).Themicroorganismsattachedtothemediainthebiofilterwereisolatedandidentified.TheresultsshowedthatidentifiedmicroorganismsarePenicilliumfrequentans,Paecilomycesvariotii,AspergilluswentiiandCephalosporiumacremonium.Keywords:biofilter;fungi;wastegas;ethylmercaptan:20030416;:20030801:(50178066):(1975),,;*:[e-mail](VOCs),VOCs3gm-3[1].,[2].,.VOCs3:.,,.,,.,[3].,,VOCs,.Woertz,175d,95%[4].pH3~6,,pH.,[5].[6].(EM).,.,150d.,,[7]..24220043ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.2Mar.,200411.11,3.1Fig.1Schematicdiagramofthebiofilterforwastegastreatment:,,...,.1m,8L,5L..1.2,25~30!().,(pH4),,80%~90%.50∀10-9,1m3h-1,,2.2,2Fig.2TheperformanceofEMremovalinthebiofilter,,(60%);,,,95%.,[8].22.113.3,.1cm3,A1,A2,A3,B1,B2,B3,C1,C2,C3.,.2.2,,10-3,02mL,.28~30!,3~5d,,,.1GyM.23[9,10],,28!4~7d,.H2S1GyM528!4d,,.,4.33424ABC191ABC13ABC15ABC192.33.1ABC191ABC1915d,,,,(MonoVerticillata).,,2~25m.,,6~10,,ABC1913,1.3ABC191(∀800)Fig.3ThefastigiatedmycelialbranchofstrainABC191(∀800)1ABC191Table1CulturalcharacteristicsofstrainABC191,,,,,,H2S,1,,,GyM,,,,,ABC191,,,,,,.,,,,ABC191(Penicilliumfrequentans).3.2ABC1328!4~5d,,ABC13,(70~180)m∀22m.(6~22)m∀(15~18)m,(4).2.4ABC13(∀800)Fig.4ThefuniformmyceliumofthestrainABC13(∀800)2ABC13Table2CulturalcharactersofstrainABC13,,,,,H2SH2S1,,GyM,,,3352:,ABC13,.,,,ABC13(Paecilomycesvariotii)..,.33ABC155ABC15(∀800)Fig.5ThesphericityconidiosporesofstrainABC15(∀800)ABC15,2~35cm,,300~500m,mm,8~18m,.,,,6m∀3m~8m∀3m.,45~5m,.5.H2S1GyM5,ABC15H2S,4,,,.,ABC15,,,,(Aspergillus).ABC15,,,,ABC15(Aspergilluswentii).3.4ABC192ABC192,,2~3m,,,,(15~30)m∀(15~3)m,,.,8~12m,4~8..,,12m∀(20~25)m.ABC1926,3.,ABC192,,,4~8,.,ABC192,ABC192(Cephalosporiumacremonium).6ABC192(∀800)Fig.6TheconidiosporesofstrainABC192(∀800)3ABC192Table3CulturalcharacteristicsofstrainABC192,,,,,H2S,,1,,GyM,,3362444Table4DistributionofmicroorganismsinthereactorA1YYYYA2YYNYA3YNYYB1YNNYB2YNYYB3YNYYC1YNYYC2YYNYC3YNNY:Y;N4.4,,,,.,,,.51GyM.,,.,.,95%.:,.:[1]VanGroenestijnJW,HesaelinkPGM.Biotechniquesforairpollutioncontrol[J].Biodegradation,1993,3(4):283301[2]DevinnyJS,DeshussesMA,WebsterTS.Biofiltrationforairpollutioncontrol[M].BocaRaton,FL,USA:CRCPressLLC,1999[3]VanGroenestijnJW,vanHeiningenWNM,andKraakmanNJR.Biofiltersbasedontheactionoffungi[J].WaterSci&Tech,2001,44(9):227232[4]WoertzJR,KinneyKA,MclntoshNDP,SzaniszloPJ.RemovaloftolueneinavaporphasebioreactorcontainingastrainofthedimorphicblackyeastExophialalecaniicorni[J].BiotechnologyandBioengineering,2001,75(5):550558[5]VanGroenestijnJW,LiuJX.Removalofalphapinenefromgasesusingbiofilterscontainingfungi[J].AtmosphericEnvironment,2002,36(35):55015508[6],.[J].,2003,4(3):15[7]DuncanKE,SubletteKL,RiderPA,SteppA,BeitleRR,ConnerJA,KolhatkarR.Analysisofamicrobialcommunityoxidizinginorganicsulfideandmercaptans[J].BiotechnologyProgress,2001,17(4):768774[8]Centerforwastereductiontechnologiesoftheamericaninstituteofchemicalengineers.Biofiltration:projectreport,scaleupanddesignguide[M].NewYork:AmericanInstituteofChemicalEngineers,1999[9].[M].:,1973[10].[M].:,19873372: