从污水微生物合成聚羟基烷酸酯

郑裕东*　钟青华　马文石(　510641)　,,,:O6311999-12-29,2000-05-17(990621)(PHA)、、,.PHA,[1,2].PHA.PHA[3],PHA,PHA[4,5].,-,、、、PHAPHA;、PHA.,,;,,;,,..1000mL,10mL(),pH7～8,3h.、,2h,25h.30min,,,4g/L,(1.2～2.8mL/min)20h.2h,,m;1∶130%,30℃3h,,PHA,、、m1[6](PHA=(m1/m)×100%).721600nm(-).,B,XPT-7.NICOLETHP5988A,;BRUKERDRX-400;NICOLETMagna-IR760.,、,PHA.PHA,PHA,-.PHA[7].、、176Vol.17No.6200012　　　CHINESEJOURNALOFAPPLIEDCHEMISTRY　　　Dec.2000Fig.1　Plotofabsorbanceofcultureliquorvsculturetime.,.、、50、88、217mg/L,0.15m3/h,,PHA11.8%.1,.,14h,,4h,,.,14h.2B.,,,,12h,,12h.Fig.2　ThemicrophotosofcellsstainedbySudanBlackBwithdifferentculturetimea.3h;b.10hPHA1HNMRW0.872,H,,2H;W1.243W1.259H,2H;W1.573H,W,;W2.423～2.476W2.556～2.613,;W5.1—CHH,,1H,1/3;W5.212～5.259H.PHAIR29292895cm-1,2929cm-1;3000cm-1CH;1723cm-1;1550～1650cm-1,CC;1378456cm-1,.-,PHA(1),PHA3-,4-,3-,3-,3--9-6616:　　,3-,3-3-,.Tab.1　PHAsproducedfromactivatedsludgebasedonGC-MSresultsRetentiontime/minLibrary/IDCrackedmonomercontent/%　　　　　　　7.22butanoicacid,butylester　　　　　　　21.449.22pentanoicacid,4-oxo,burylester5.3316.09propyldodecanoate3.9618.20propyltetradecanoate0.9019.959-hexadecenoicacid,methylester14.0020.31propylhexadecanoate24.5021.6911-octadecenoicacid,methylester19.8421.749-octadecenoicacid,methylester5.3321.90octadecenoidacid,propylester4.70　　　　1　LefebvreG,RocherM,BrauneggG.Applied&EnvironMicrobio,1997,163(3):827　2　YamaneT,ChenXF,UedaS.ApplEnvironMicrobiol,1996,62(2):380　3　(CHENQi),(HUANGHe-Rong),(YIZu-Hua).微生物学通报(WeishengwuxueTongbao),1994,21(5):297　4　SatohH,IwamotoY,MinoT,etal.WatSciTech,1998,38(2):103　5　SatohH,MinoT,MatsuoT.WatSciTech,1992,26(5):933　6　HalmSK,ChangYK,KimBS,etal.BiotechnolBioeng,1994,44:256　7　(ZHENGYuan-Jing),(SHENGuang-Fan),(WUYang-Shan)(Chief-ed).(TreatmentofWasteWaterbyBiomembrane),(1st),(Chapt4).(Beijing):(ChinaBuildingIndustryPress),1983BiosynthesisofPolyhydroxyalkanoatesfromWasteWaterZHENGYu-Dong*,ZHONGQing-Hua,MAWen-Shi(InstituteofMaterialScience,SouthChinaUniversityofScienceandTechnology,Guangzhou510641)Abstract　Anumberofmicroorganismsinactivatedsludgeintheprocessofwastewatertreatmentwereusedforaccumulatingpolyhydroxyalkanoates(PHA).Mixedbacteriaandorganiccarbonsourcewereusedinmicroaerophilic-aerobictreatmentofactivatedsludgeandsomeculturalconditions:oxygensupplyrate,attachmentcarbonsourceconcentrationandculturetimewereinvestigated.ThePHAsobtainedappearedamixtureofcopolymersorheteropolymerswithvariousmonomerunites:3-hydroxybutyrate,4-hydroxy-pentanoate,3-hydroxydodecanoate,3-hydroxy-tetradecanoate,3-hydroxy-9-hexadecanoate,3-hydroxyhe-xadecanoate,3-hydroxy-11-octadecanoate,3-hydroxy-9-octadecanoate,and3-hydroxyocta-decanoate.Keywords　polyhydroxyalkanoate,biosynthesis,wastewater,activatedsludge662　　　　　　　　　　　　　　　　17