大气压等离子体射流对水中铜绿微囊藻的灭活作用

94Vol．9No．420154ChineseJournalofEnvironmentalEngineeringApr．2015*214122APPJAPPJpHH2O2O3APPJ。7kV4L/min0．20099．16%H2O2O3NO－3NO－2pH。H2O2O3pHH2O2O3pHAPPJ。X172A1673-9108201504-1651-08InactivationeffectofMicrocystisaeruginosainwaterbyatmospheric-pressureplasmajetXieJingHaoXiaolongZhuBailinSchoolofEnvironmentalandCivilEngineeringJiangnanUniversityWuxi214122ChinaAbstractTakingMicrocystisaeruginosaM．aeruginosaastheresearchobjectanovelatmospheric-pres-sureplasmajetAPPJaqueousreactionsystemwasbuiltthroughairsourceAPPJintroducedintoliquidphase．WeinvestigatedtheeffectsofthedifferenttreatmentmethodsofAPPJontheinactivationefficiencyofM．aerugi-nosaobservedthechangeofalgalcellmorphologybydischargetreatmentanalyzedtheeffectsofmainactivesub-stancesH2O2O3generatedintheliquidandpHchangeonalgalinactivationanddiscussedthemechanismofM．aeruginosainactivation．Experimentalresultsshowedthattheinactivationratereached99．16%underthevolt-ageof7kVtheairgasflowrateof4L/minandtheOD680ofabout0．200．ThetreatedalgalcellsweredeformedandrupturedandactivesubstancesH2O2O3wereproducedinalgalsolutiontreatedbyAPPJmeanwhilelargenumbersofionsNO－3NO－2wereproducedresultingintherapiddecreaseofaqueouspH．Bytheinves-tigationofthefactorsofmainactivesubstancesH2O2O3andpHinalgalmediumitwasfoundthatalgalinac-tivationeffectwasnotbetterbyasinglefactorbutthecombinedfactorsofmainactivesubstancesH2O2O3andpHwerethemainreasonsforM．aeruginosainactivationinwaterbyAPPJ．Keywordsatmospheric-pressureplasmajetMicrocystisaeruginosaactivesubstanceinactivationeffect2110703421376108“”2012ZX07101-013-042014－03－132014－03－151984—。[e-mail]*[e-mail]。M．aerugi-nosa1。、2-6、、7-9、10-13。。atmospheric-pressure9plasmajetAPPJ。APPJ、、、、14-17。AkiyamaH．18B-PFNBaiMindong19100%APPJ。APPJ2021、·OH、·O、O－2、O*2O31922232425。APPJ27-29。APPJAP-PJ、。11．1M．aeruginosaFACHB-924。BG1125±1℃12h∶12h2500lux。1．21、、。APPJ2。0．45μm2～8L/min。0．3L。0～220V0～15kV0～10kV。10kΩ1APPJFig．1SchematicdiagramofAPPJgeneratedinaqueoussolution2APPJFig．2StructureanddischargeimageofAPPJ。1．3100mLOD6800．200pH10．00±0．30aChl-a200mg/L。7kV30min4L/min。OD680、pH4d3H2O2、O3、NO－2NO－3。1．3．1100mL0．45μm25614BG11。1．3．2O3、H2O2pH100mLpH10.00±0．30H2O2O3AN-SEＲOSCOM-AD-01O3。A．0．25mg/LO330min30minB．10．64mg/LH2O2C．0．25mg/LO330min10．64mg/LH2O2。pHpH4．2A．pH4．2B．pH4．20．25mg/LO330minC．pH4．210．64mg/LH2O2D．pH4．20．25mg/LO330min10．64mg/LH2O2。1．4OD680680nmOD680η=A0－AA0×100%1A0A。SEM。50mL4000r/min15min2．5%24h1%2h。10%、30%、50%、70%、90%、100%30。H2O2K2TiOC2O42·2H2O31。32。、33。22．1APPJ、、、、3334。APPJ1．3．13。3276．91%278．44%2597%。APPJ。3Fig．3InactivationratesofM．aeruginosawithdifferenttreatmentmethods2．2SEMAPPJSEM2d。44a。30min4b。2d4c35619。APPJ。2021·OH、·O、O·－2、O*2、O335。4Fig．4ScanningelectronmicrographsofalgalcellularmorphologyunderAPPJexposure2．3APPJH2O2、O3、HNO2HNO333。2．3．1pHAPPJpH530minpH10．24．26．0pH30min4．254．80．6。APPJpHpH。5pHFig．5ChangesofpHwithdifferentincubationtimesAPPJpH6APPJ。620min20min40min80．68mg/L42．52mg/L2。N2NN+2OO2NONO236-39HNO2HNO333N2+e→2N·+e2N·+·OH→NO+H+3NO+·OH→NO－2+H+4NO－2+H2O2→NO－3+H2O5NO2+NO2+H2O→NO－2+NO－3+2H+6NO+NO2+H2O→2NO－2+2H+7NO－2+O3→NO－3+O2856NO－3·OH、H2O2O3NO－220min·OH、H2O2O345614NO－2NO－3。6Fig．6VariationsofconcentrationsofNO－2andNO－3withtreatmenttimeofAPPJ2．3．2H2O2、O3APPJH2O2O3。H2O2·OH·OHH2O38-412。H2O2·OH。H2O+e→H++·OH9·OH+·OH→H2O210O3OO2O333O2+e→O+O+e11O+O2→O312APPJ30minH2O2、O310．640．53mg/L。2．4APPJAPPJO3、H2O2pH。、pHAPPJ。2．4．1O3、H2O22．3．2O3、H2O27pHO3AH2O2B5A、B51．35%58．98%H2O2O3O3H2O2C5C92．34%APPJ30minD599．16%。O3H2O2H2O2O3H2O2O3H2O2。O3H2O2O3H2O2Peroxone·OH·OHAPPJAPPJ17pHpH。A．0．25mg/LO330minB．10．64mg/LH2O2C．0．25mg/LO330min10．64mg/LH2O2D．APPJ30min7Fig．7EffectofactivesubstancesoninactivationratesofM．aeruginosa2．4．2pH2．3．2pH8。pHA548．34%pH4．2O3BH2O2C5BC90．32%87．43%。O3H2O2D5d94．30%APPJ99．16%。pHO3H2O2pH、O3H2O23。55619APPJ。APPJ·OH、O·－2、O*2、O3H2O2NONO2HNO2HNO3。A．pH4．2B．pH4．20．25mg/LO330minC．10．64mg/LH2O2pH4．2D．pH4．20．25mg/LO330min10．64mg/LH2O2E．APPJ30min8pHFig．8EffectofpHchangeoninactivationratesofM．aeruginosa3APPJ4L/min7kV30min99．16%pH、、10．64mg/L0．53mg/L。APPJ·OH、O3、H2O2、pH。1．-．200733723-26FanXuehongWangQishanLiuShanpeietal．Effectofpre-oxidationonMicrocystisaeruginosaremovalbycoagula-tion-DAFprocess．Water＆WastewaterEngineering200733723-26inChinese2．．200425640-43ZouHuaPanGangChenHao．FlocculationandremovalofwaterbloomcellsMicrocystisaeruginosabychitosan-modi-fiedclays．EnvironmentalScience200425640-43inChinese3．-．2010301258-61GaoShanshanZhangHuiYangJianyuetal．Optimizationofparametersintheelectrocoagulation-electroflotationprocessforremovingalgae．IndustrialWaterTreatment2010301258-61inChinese4．PAC．2011591979-1983XuXiaojieWuChundeDongQietal．Enhancedcoagula-tionofslightlypollutedalgae-containingsurfacewaterwithcombinationofPACanddiatomite．ChineseJournalofEnvi-ronmentalEngineering2011591979-1983inChi-nese5．．2005262148-151ZouHuaPanGangChenHao．Effectsofionicstrengthontheflocculationandremovalofcyanobacterialcellsofmi-crocystisaeruginosabyclays．EnvironmentalScience2005262148-151inChinese6．．2013793312-3318JiangShaojieJiangHuiXiangPingetal．Ｒemovalofsynedraacusbyenha