低溶解氧和磷缺乏引发的非丝状菌污泥膨胀及控制

1,1＊,2,3,4(1.,　150090;2.,　523581;3.,　518055;4.,　100085):,、.(0～0.7mgL)PBOD5(0.78100)2.,,SVI.PBOD5,.,,,,NaClO.:;;;;:X703.1　:A　:0250-3301(2007)03-0545-06:2006-04-04;:2006-06-23:(1973～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]-filamentousSludgeBulkingCausedbyLowConcentrationofDissolvedOxygenandPhosphorusDeficiencyandItsControlWANGJian-fang1,ZHAOQing-liang1,LINJi-kan2,JINWen-biao3,XIAOBen-yi4(1.SchoolofMunicipal&EnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China;2.GoldenIdeaBio-TreatGroup,DongguanGuangdong523581,China;3.HarbinInstituteofTechnologyCampusofShenzhenUniversityTown,Shenzhen518055,China;4.ResearchCenterforEco-EnviromentalSciences,ChineseAcademyofSciences,Beijing100085,China)Abstract:Non-filamentoussludgebulkingoccurredduringsludgeacclimationinlab-scalebio-reactorsandthecharacteristics,propertiesanddegradingpollutantscapabilityofnon-filamentoussludgewereanalyzed.BothlowDOconcentration(0～0.7mgL)andlowratioofPBOD5(0.78100)resultedinexcessiveproliferationofviscouszoogloeaandsludgebulking.SVIwasdirectlyproportionaltoextracellularpolysaccharidesandwasinverselyproportionaltohydrophobicityofsludge.ThesludgebulkingcouldbeeffectivelycontrolledbyincreasingDOconcentrationandPBOD5ratio.Besides,addingsomeporouspaddingintoviscousbulkingsludgewasausefulmethodwhichmightavoidthedeteriorationofsludgesettlementandmaintaingoodperformanceinthesystem.TheviscousbulkingsludgecouldnotbecontrolledbyadditionofNaClO.Keywords:non-filamentousbulking;hydrophobicity;extracellularpolysaccharides;dissolvedoxygen;phosphorusdeficiency　　1,.,2.95%[1];(viscousbulking)(zoogloeabulking),,,.,,,[2～4],、,,.SBR,、,,.1　1.1　,A、B2(8.6cm,48cm),2.4L.SBR.6h,28320073　　　　　　ENVIRONMENTALSCIENCEVol.28,No.3Mar.,2007DOI:10.13227/j.hjkx.2007.03.018,2h,3.5h(),0.5h.2.20℃.,SVI70～90mLg.SBR3000mgL,0.63kg(kg·d),SRT10d.1.2　,:CH3COONa0.8gL,NH4Cl0.2gL,K2HPO40.0125gL,KH2PO40.0125gL,CaCl20.012gL,MgSO4·7H2O0.0125gL,FeSO4·7H2O0.01gL,1mL.:N(CH2COOH)31.5gL,MnSO4·H2O0.38gL,CuSO4·5H2O0.01gL,H3BO30.01gL,Na2MoO40.01gL,KAl(SO4)2·12H2O0.03gL,CoCl2·6H2O0.1gL,NaCl0.1gL.:COD632mgL,BOD5365mgL,NH+4-N43.49mgL,TN53.51mgL,TP2.8mgL,pH7.5.1.3　CODHACH;BOD5、NH+4-N、TN、TP、MLSS、MLVSSSV30;OURDOYSI5100;OLYPUSBX51.-[5],-[6].[7]:3500rmin15min;-(PUM,pH7.0)2;PUM,540nm,(2.0～2.5),Ai;4mL,1mL,30℃15min;1min,30min,Af.:(%)=Ai-AfAf×1002　2.1　2.1.1　1d,A、BSBR.,,SVI75～95mLg.1,,COD80%,.,SVI,20,2.,,,,SV3090～99.,SVI1.,3.0Lmin,DO2～3mgL.,,DO0～0.7mgL,BDOADO,BSVIASVI.10～48h,,.,,SS10～50mgL.,Yobs0.56,Yobs0.45.1　SVIFig.1　FluctuationofSVIversusrunningtime　,,.,、60%,,.10,2.,、、,0.1%～1%.16SrRNA,10%[7].546　　　　　　28　　　　　　2　Fig.2　Phasecontrastmicrographsoftheactivatedsludgezoogloeainnon-filamentousbulkingsludge　3　SVIFig.3　EffectofSVIonremovalefficiencyofCOD,NH+4-N,TN,TP,、Z.ramigera,Thauerasp.[8].2.1.2　A、B,,SVICOD、NH+4-N、TN、TP3.,A、B.SVI200～350mLg,CODTP80%;NH+4-NTN,2.NH+4-NTN85%80%60%65%.,3.0Lmin,DO0～0.7mgL.,,,,.2.1.3　.Z.ramigera,.5473:　　、、,,.,,.,.,[7].、、、,1.,214～12,22.SVI,,,SVI,.,,.1,,BSVI346mLg,MLVSSSOUR1.,;,,.1　SVI、SOUR、、1)Table1　AnalysisofSVI,SOUR,hydrophobicityandpolysaccharidesinEPSofvarioussludgeSVImL·g-1SOURmg·(g·h)-1%mg·g-1A26591.7226.61339B34694.4114.983989244.4251.421677862.9568.381311)2.2　,[9,10]:(,、);;;、;.,SBR.,SBR,,、、,.2SBR,、、、,.,2..3000mgL,,0～0.7mgL.,.,,PBOD50.78100.,,Zoogleasp.Thauerasp.,,,,,.[11]BOD5P1000.8,;BOD5P1000.61000.3,,;BOD5P1000.4,.2.3　、,,,[10,12].,PBOD5、SBR;、NaClO.2.3.1　PBOD5SBRA、B2PBOD5,SVI4.548　　　　　　284　SVIFig.4　VariationofSVIinthecourseofsludgebulkingcontrol　A,;B,PBOD51100,,SVI.PBOD5.,20,A、BSVI,,21,PBOD5,2SVI.55,SVI.100,2.2.10,100,、.2.3.2　,(3).,PBOD5,(3500mgL)1L,(12mm×12mm×18mm,95%,),60L,.6,,,SS,5.,5　Fig.5　FluctuationofremovalefficiencyofCOD,TN,TP,SSinthecourseofsludgebulkingcontrolbyaddingporouspadding　,.,,,,.,、2mgL、(BOD5∶N∶P100∶5∶1),1,,、、,.2.3.3　NaClOVanleeuwen[13].NaClO,,NaClO.,NaClOMLSS41.65μgg83.3μgg,,;16.66μgg,,COD85%39%,,SS150mgL.,、.,NaClO,NaClO,,,,.5493:3　　　(1)SBR,,COD、TP80%,NH+4-N、TNSVI..(2)1,14～12.,,,.(3)PBOD52.,.2mgL、PBOD51100,100,.(4)12mm×12mm×18mm60L,,.NaClO.NaClOMLSS41.65μgg,;NaClO16.66μgg,,COD85%39%,SS150mgL.:[1]PengY,GaoC,OzakiM,etal.Non-filamentoussludgebulkingcausedbyadeficiencyofnitrogeninindustrialwastewatertreatment[J].WaterScienceandTechnology,2003,47(11):289～295.[2],.BIOLAK[J].,2004,30(7):56～57.[3],,.SBR[J].,2003,19(5):100～101.[4],,,.[J].,2004,20(2):101～103.[5]LiuH,FangHHP.Extractionofextracellularpolymericsubstances(EPS)ofsludges[J].JournalofBiotechnology,2002,95:249～256.[6]DuboisM,GillesKA,HamiltonJK,etal.Colorimetricmethodfordeterminationofsugarsandrelatedsubstances[J].AnalyticalChemistry,1956,28:350～356.[7]GabrielB.Wastewatermicrobiology[M].(2nded).NewYork:JohnWiley&Sons,Inc.,1999.181～232.[8]MichealSA,KarenTW,BenjaminSP,etal.Analysisandglycosylcompositionoftheexopolysaccharideisolatedfromthefloc-formingwastewaterbacteriumThauerasp.MZ1T[J].EnvironmentalMicrobiology,2004,6(8):780～790.[9],,,.SBR[J].,2005,25(1):105～108.[10]NovakL,LarreaL,WannerJ,etal.Non-filamentousactivated-sludgebulkingcausedbyZoogloea[J].WaterScienceandTechnology,1994,29(7):3