低温促进反硝化颗粒污泥稳定性的研究张云辉

张云辉,　杨珊珊,　金锡标,　王　锋,　别红梅(华东理工大学环境工程研究所,上海200237)　　　:　针对高负荷下出现的反硝化颗粒污泥相互粘连、上浮等不稳定状态,进行了降低进水温度促进颗粒污泥床稳定的研究。当进水温度降至16℃时反应器能稳定运行,颗粒粘连现象减弱,颗粒密度由不稳定时的1.0085g/cm3提高到1.022g/cm3,颗粒沉速为30～50m/h,此时反应器的负荷为4.0～5.14gNO-3-N/(L·d),对氮的去除速率为0.18gNO-3-N/(gVSS·d);当进水COD和NO-3-N浓度分别为225mg/L和50mg/L时,对其去除率分别为93%和98%。研究认为,颗粒污泥表面反硝化菌的生长速率过快是引起不稳定的主要原因,降低温度即降低微生物的生长速率有助于颗粒污泥保持稳定。　　:　反硝化颗粒污泥;　稳定性;　低温:X703　　:A　　:1000-4602(2009)11-0025-04EffectofLowTemperatureonStabilityofDenitrifyingGranularSludgeZHANGYun-hui,　YANGShan-shan,　JINXi-biao,　WANGFeng,　BIEHong-mei(ResearchInstituteofEnvironmentalEngineering,EastChinaUniversityofScienceandTechnology,Shanghai200237,China)　　Abstract:　Tosolveproblemssuchasmutualadhesionandfloatingofdenitrifyinggranularsludgewheninfluentloadsarehigh,thetestofloweringinfluenttemperaturetostabilizegranularsludgebedwasperformed.Whentheinfluenttemperaturedropsto16℃,thereactorcanstablyoperateandthegranuleadhesionisweakened.Thesludgedensityisincreasedfrom1.0085g/cm3measuredunderunstablecon-ditionto1.022g/cm3measuredaftertemperatureadjustment.Thesludgesettlingvelocityis30m/hto50m/h.Atthistime,thereactorloadingrateis4.0to5.14gNO-3-N/(L·d),andthenitrogenre-movalrateis0.18gNO-3-N/(gVSS·d).WhentheinfluentCODandNO-3-Nconcentrationsare225mg/Land50mg/L,theirremovalratesare93%and98%respectively.Thegranuleinstabilityismainlycausedbyfastgrowthrateofdenitrifyingbacteriaonthegranules.Reducingthetemperature,thatis,loweringthegrowthrateofmicroorganisms,contributestothestabilityofthegranularsludge.　　Keywords:　denitrifyinggranularsludge;　stability;　lowtemperature　　:“”(2008BAE64B06)　　,[1～3]。,,[2]。,、,。[4],[5～7]。Zafar·25·25　1120096　　　　　　　　　　　　CHINAWATERWASTEWATER　　　　　　　　　　　　　Vol.25No.11Jun.2009[8],。,,,。:,;(),。,。1　材料与方法1.1　1。1　Fig.1　Schematicdiagramofexperimentalequipment(USB),60mm,1000mm,2.8L,5,200mm。1.2　、,,COD/NO-3-N4.5;,1/10。1.3　COD、NO-3-N、NO-2-N;(SEM);MLSS、MLVSS。2　结果与讨论2.1　(VSS/SS=0.60),28g(SS),26℃。NO-3-N50mg/L,0.5gNO-3-N/(L·d)4.0gNO-3-N/(L·d),40d,。、,2～3mm,MLSS32g/L。4.0gNO-3-N/(L·d),0.15gNO-3-N/(gVSS·d)0.7gCOD/(gVSS·d),100%。16d,4.0gNO-3-N/(L·d)6.44gNO-3-N/(L·d)0.32gNO-3-N/(gVSS·d),,3～5mm,,;5～8mm,,,,。:,,,。,。1,、、、。1　Tab.1　Physicalandchemicalcharacteristicsofgranularsludge　(80%)/mm2～33～5/(g·cm-3)1.02791.0085/(m·h-1)35～6025～40VSS/SS0.750.80/%25202.2　2.2.1　1,5.14gNO-3-N/(L·d),22、19、16℃(10、10、28d),。·26·25　11　　　　　　　　　　　　　　　　　　　　　　　　　　℃,7,;19℃,,,15,3～5mm,,;16℃,,,,,,,。(4.3m/h),,,,,。MLSS30g/L,0.18gNO-3-N/(gVSS·d)0.8gCOD/(gVSS·d),NO-3-NCOD3mg/L30mg/L。,,。16℃3～5mm,,1.022g/cm3,30～50m/h,24%;。2.2.2　2,4.0gNO-3-N/(L·d),20℃,20d2～4mm,1.0228g/cm3,33～50m/h,VSS/SS0.72,MLSS28g/L30.9g/L,NO-3-NCOD0.183gNO-3-N/(gVSS·d)0.795gCOD/(gVSS·d)。,。,16℃20d,,33g/L;52d2～4mm,1.0219g/cm3,VSS/SS0.73,。(≤20℃),,,。,NO-3-NCOD95%90%,。16℃1,,,,,。2.3　,,,,,,。,,。22℃,,;20℃,,,,,。,,,COD。,。,,,,26℃。,、,,。。,7.5d,3.73d,6.1d;(16℃),6.6d。,。3　结论,,。,。:[1]　HiscockKM,KloydJW,LernerDN.Reviewofnatural(下转第32页)·27·:25　1191.86%。③　C/NMUCT,0.13～0.79mg/L,98.83%。:[1]　BortoneG,MarsiliLS,TilcheA,etal.AnoxicphosphateuptakeintheDEPHANOXprocess[J].WaterSciTechn-ol,1999,40(4-5):177-185.[2]　WachtmeisterA,KubaT,vanLoosdrechtMCM,etal.Asludgecharacterizationassayforaerobicanddenitrifyingphosphorusremovingsludge[J].WaterRes,1997,31(3):471-478.[3]　,,,.、[J].,2003,19(1):33-36.[4]　KubaT,vanLoosdrechtMCM,HeijnenJJ.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WaterRes,1996,30(7):1702-1710.[5]　KubaT,vanLoosdrechtMCM,BrandseFA,etal.Oc-currenceofdenitrifyingphosphorusremovingbacteriainmodifiedUCT-typewastewatertreatmentplants[J].Wa-terRes,1997,31(4):777-786.[6]　ФstgaardK,ChristenssonM,LieE,etal.Anoxicbiologi-calphosphorusremovalinafull-scaleUCTprocess[J].WaterRes,1997,31(11):2719-2726.[7]　HaoXiaoDi,vanLoosdrechtMCM,MeijeiSCF,etal.Model-basedevaluationoftwoBNRprocesses—UCTandA2N[J].WaterRes,2000,35(12):2851-2860.[8]　,.[M].:,2003.[9]　PengYZ,WangXL,LiBK.Anoxicbiologicalphos-phorusuptakeandtheeffectofexcessiveaerationonbio-logicalphosphorusremovalintheA2Oprocess[J].De-salination,2006,189(1-3):155-164.:(1954-　),　,　,　,　,　。:[phone]　13756568756E-mail:[e-mail]:2008-12-19(上接第27页)　andartificialdenitrificationofgroundwater[J].WaterRes,1991,25(9):1099-1111.[2]　,.[J].,2001,17(4):23-26.[3]　,,,.[J].,2007,23(11):8-12.[4]　,,,.[J].,2006,7(8):52-56.[5]　FrancoA,RocaE,LemaJM.Granulationinhigh-loaddenitrifyingupflowsludgebed(USB)pulsedreactors[J].WaterRes,2006,40(5):871-880.[6]　WangXH,ZhangHM,YangFL,etal.Improvedstabil-ityandperformanceofaerobicgranulesunderstepwisein-creasedselectionpressure[J].EnzymeMicrobTechnol,2007,41(3):205-211.[7]　SeanConnaughton,GavinCollins,VincentO'Flaherty.Developmentofmicrobialcommunitystructureandactivi-tyinahighrateanaerobicbioreactorat18℃[J].WaterRes,2006,40(5):1009-1017.[8]　ZafarIqbalBhatti,KenkoSumida,JosephDRouse,etal.Characterizationofdenitrifyinggranularsludgetreatingsoftgroundwaterinanupflowsludge-blanketreactor[J].JBiosciBioeng,2001,91(4):373-377.:(1983-　),　,　,　,　。:(021)6