低温好氧反硝化菌群强化生活污水脱氮效能庞长泷

4612201412JOUＲNALOFHAＲBININSTITUTEOFTECHNOLOGYVol.46No.12Dec．2014庞长泷1，2，陈翰1，李昂1，马放1，21．1500902．21420010℃、．10.31%～16.89%25.07%～32.44%A10d、、CODCr2.433.076.023.63mg/L．X703.1A0367－6234201412－0045－08PerformanceofbioaugmentednitrogenremovalbyaerobicdenitrficationconsortiumfortreatingthedomesticsewageatlowtemperaturesPANGChanglong12CHENHan1LIAng1MAFang121．StateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentHarbinInstituteofTechnology150090HarbinChina2．HITYixingAcademyofEnvironmentalProtection214200YixingJiangsuChinaAbstractLowtemperatureinhibitstheperformanceofnitrogenremovalefficiencyduringthebiologicaltreatmentofmunicipalwastewater．Thereforetheeffluentcouldhardlymeettheemissionstandard．Toenhancetheperformanceofnitrogenremovalatlowtemperatureapsychrotolerantaerobicdenitrificationconsortiumbyfastenrichmentanddomesticationwasobtainedwhichcouldremovetheammonianitrateandorganicmattersimultaneouslyandefficientlyinaerationenvironmentunder10℃．Theresultsshowedthattheremovalefficiencyofammoniumandtotalnitrogenbybioaugmentationwasimprovedby10.31%－16.89%and25.07%－32.44%respectively．Itmadetheeffluentqualitymeetthegrade1Alevelofnationaldischargestandard．Afterbioaugmentationterminatingfor10daystheremainedconsortiumcouldstillimprovethereactorthattheeffluentofammoniumnitratetotalnitrogenandCODCrconcentrationswasdecreasedabout2.433.076.02and3.63mg/Lrespectively．Theresultsindicatedthatbioaugmentationperformedwellandpersistently．Keywordslowtemperaturedomesticsewagenitrogenremovalbioaugmentationaerobicdenitrification2014－01－12．5110812051178139．1983—1963—[e-mail]．、1．2－3．4－7、8－10．．10℃SBＲ、．11.1．、、、111．2．0.018g/cm3100～120m2/g1～2mm2cm×2cm×2cm．14～16．1mg·L－1TOCpH80～1000～5140～160230～270750～9507.0～7.52mg·L－1CODCrpH100.0～300.035.0～55.00～0.50～3.040.0～60.06.8～7.21.210℃、、、3.0L1∶3．24h5min23h50min5min．SBＲ10℃．SBＲ、、、10.0L1．!!#$%!&!’!()*!#$%!+,-./’0//1234567&+,-./’&!#$%&()*&&!’&#$%1SBＲ1.31．3．130min0.6L2.4L．8000r/min10min、、、TOC3．、．1．3．2SBＲSBＲ8h4.0L40%．10℃·64·46MLSS3000～3500mg/L20d．MLSSSV．A/O/A0.5h4h2h1h．、1min28min．、、．、．1．3．312．6～8d．2#1#．．2～4mg/L．．．1.4CODCr、TOC、、、、、pH、DOMLSS13．1.5MicrosoftOfficeExcel2010OriginLabOrigin8.0．22.1TOC．TOC10.98mg/L．．．1192.03%39.98mg/L．10d10d．2．1082.85%21.09mg/L．!#!$#!%#!&#!###$#%#&#!#$’(!###$#%#&##%&’()’)!*%&+’*+,-./!0,-%&.-%&%&’()#&%$!#22d3.823.14mg/L0mg/L．．14．12d、．2.210℃SBＲ45dCODCr·74·12．2.2.13．30.46mg/L．1710.62mg/L78.36%2184.78%6.43mg/L22～35d3681.09%6.56～8.65mg/L、．．．!#$%#$&’(!#$%$&#)*+,’(%)!#$#$&’(’*!+#$,’+-./01%,3SBＲ2.2．2．．4SBＲ1～6d7～13d13d13.91mg/L1425．3910.71～11.25mg/L．!!#$%$!&##$#!#’##!#$()%&’()(*+,-./$0*+%’()+%’()4SBＲ2.2.35．16d34.05～41.76mg/L17～36d21.87～31.55mg/L3761.29%18.54～19.76mg/LA．!#$%#$&’(!#$%&’’&%$)*+,()*!$&!+#$,(+-./01’,#$&’((25SBＲ3d·84·46CODCr88.27mg/LCODA4CODCr4169.97%CODCr39.07～46.23mg/LA．CODCr．SBＲ．2.3SBＲ2#SBＲ1#SBＲ．2.3.16．1#7.41～10.22mg/LA2#．9d2#7.87mg/L95.22mg/L2#11～30d2#3.01～4.21mg/L213.01mg/L．．2116.89%272#93.35%．10.31%～16.89%．!##$%&’(#!#$)*%&’()*+,&’(*+-,&’(!+’(,)+-./01(,’(’’$’6SBＲ．2.3.21#2#9d90.45mg/L200mg/L．．7．1#11.14～14.48mg/L2#．2#177.31mg/L1#248.22mg/L2#6.02mg/L281#12.03mg/L．2#15．．!!#!$%&$!$#$’$!#$()%&’()(**+,-.!+,-.%&’()-./%&’()7SBＲ2.3.3·94·128．1#21.11～25.44mg/LA．2#．112#13.48mg/L16～30d2#10.77～11.76mg/L．1#44.04%～55.62%2#1575.19%1#50.38%2432.44%2#2778.59%．2#25.07%～32.44%．!#$###%&’&$#%(’($%!(%(!%!&%!#$)*!+%&,)+-./01(,’(%&)*+,(%&*+-,(%&8SBＲ272#．270.142g1#2#3.5013.062g/L2#4.127g/L0.9871.215mg·g－1·h－1．2#．1#CODCr41.44～51.62mg/LCODACODCr41.44mg/L25．2#CODCr146.93mg/L940.43mg/L1037.12～38.76mg/LCODCr37.12mg/L18．2#CODCr1#2#．CODCr2#CODCr8CODCr72.22%16CODCr76.55%CODCr8.18%．2.3.428．9、10．、CODCr0h40%0.5h0.5h．91#2#6.02～8.48mg/L．．101#0.64mg/L2#CODCr2#2.58mg/L1#．．·05·46．!#$%&&%$#!’()!#$*+!,%&-*,./012&-’()*%’()+,%’(),%()-*%()-+,%()-,%928!#!$#!%#!&#!###$#%#&###!&’%($)!#$*+)#$#(#%#’#&#!##!,%&-*,./012!-345*,./012!-’()%&()*%&’()345()*34510282.3.540.00mL+160.00mL、40.00mL+160.00mL+440.00mL+160.00mL+4500.00mL1、2、310℃150r/min．3d141.97±0.55mg/L38.15±0.17mg/L233.67±0.32mg/L326.28±0.59mg/L．123．2.430d2#10d．1#．2#．30、5103．2#1#2#10d2#1#、、CODCr2.433.076.023.63mg/L．3mg·L－1CODCr30d1#9.29±0.61012.99±2.9924.01±0.7942.26±7.162#3.95±0.2406.26±3.8211.42±0.1638.29±3.615d1#10.25±0.63012.58±0.8724.56±0.4948.57±1.592#5.98±0.1907.98±1.6415.17±0.5940.25±6.5110d1#8.64±0.31012.34±1.3422.71±0.2746.08±1.952#6.21±0.2709.27±2.4316.69±0.9542.45±2.31·15·12．3110℃、．21#2#10.31%～16.89%25.07%～32.44%A．310d．1#2#、、CODCr2.433.076.023.63mg/L．4．1．M．3．2007．2HEADMAOLESZKIEWICZJA．BioaugmentationfornitrificationatcoldtemperaturesJ．WaterＲesearch2004383523－530．3．J．200925182－8588．4．J．2008285885－891．5．SBＲJ．2009298844－849．6．SBＲJ．200733513－17．7．AOJ．200622884－88．8．J．2013332286－292．9．J．200941276－80．10．J．20102981567－1570．11YAOShuoNIJinrenCHENQianetal．EnrichmentandcharacterizationofabacteriaconsortiumcapableofheterotrophicnitrificationandaerobicdenitrificationatlowtemperatureJ．BioresourceTechnology2013127151－157．12．D．2011．13．M．4．2003．14TＲEUSCHAHLEININGEＲSKLETZINAetal．Novelgenesfornitritereductaseandamo-relatedproteinsindicatearoleofuncultivatedmesophiliccrenarchae