短程硝化启动运行中功能菌群变化研究陈晓轩

MAY20,2012,39(5):597605MicrobiologyChina©2012byInstituteofMicrobiology,[e-mail](No.50878072,51008111);(No.E2009000709)*Tel:[phone];:[e-mail]2011-10-10;2011-12-12*(050018):-,(SBR),DO(1mg/L),(NOB)(AOB),0.055kg/(m3·d),0.043kg/(m3·d)0.16kg/(kg·d),83.4%,NOB2.0×105CFU/mL1.5×104CFU/mL,5.51%2.14%;AOB4.5×104CFU/mL1.5×107CFU/mL,0.18%7.25%AOB,:,AOB,NOBVariationoffunctionalbacteriaduringstart-upandoperationofpartialnitrificationprocessCHENXiao-XuanLIUChunYANGJing-Liang*ZHANGRunYANGHui-Na(SchoolofEnvironmentalScienceandEngineering,HebeiUniversityofScienceandTechnology,Shijiazhuang,Hebei050018,China)Abstract:[Objective]Partialnitrification-anammoxisconsideredastheshortestprocessforDOI:10.13344/j.microbiol.china.2012.05.0015982012,Vol.39,No.5[Me-thods]Thevariationoffunctionalbacteriaduringstart-upandstableoperationofpartialnitrifi-cationprocesswasinvestigatedinaSBRbioreactorinthisstudy.[Results]Theresultsindicatedthatammonia-oxidizingbacteria(AOB)populationwasexpandedsignificantlyandni-trite-oxidizingbacteria(NOB)populationwasinhibitedwhenDOconcentrationwascontrolledlowerthan1mg/Landammonialoadingoftheinfluentincreasedgradually.Asaresult,start-upandstableoperationofpartialnitrificationprocesswasrealized.Whenammoniavo-lumetricloadingoftheinfluentwas0.055kg/(m3·d),theaverageammoniaremovalvolumetricloadingandsludgeloadingwere0.043kg/(m3·d)and0.16kg/(kg·d),respectively.Inaddition,theaveragenitriteaccumulationratewas83.4%atthistime.AOBpopulationdensityandrel-ativeabundanceincreasedfrom4.5×104CFU/mLto1.5×107CFU/mLandfrom0.18%to7.25%,respectively,duringstart-upandstableoperationofpartialnitrificationprocess.Atthesametime,NOBpopulationdensityandrelativeabundancedecreasedfrom2.0×105CFU/mLto1.5×104CFU/mLandfrom5.51%to2.14%,respectively.[Conclusion]TheexpansionofAOBpopulationwasresponsibleforrealizationofpartialnitrificationandammoniaremoval.Highammoniacon-centrationandloadingalsocausedtheactivityinhibitionofpartialnitrification.Keywords:Partialnitrification,AOBpopulation,NOBpopulation-,/,,,,,-,:(AOB),,;(NOB),,,[1],,,,,DOpH(SRT)[2−6]DOAOBNOB5,DO0.7−1.4mg/L,90%[2−4],NOBpH6.0−7.5;AOBpH7.0−8.5[5]pH:AOBpH;pH,NOB,pH7.58.5NOB[6]MPN,FISH[7][8]MPNSBRAOBNOB1031[9]FISHSBRAOB8%−9%,NOB0.5%NOBAOB,,AOB,[10−12]SBRDO,NOBAOB,,/:599[13],,,118d,4,11.21.2.1:(1)FISH250μL1.5mL,34%4°C1h,10000r/min5min,,1×PBS3,,50%80%100%,3min1μL0.1nmol/μL(2)9μL(0.9mol/LNaCl,0.01%SDS,0.02mol/LTris-HCl,,pH7.2),,(UVP,HB-1000,),46°C4h46°C(0.01%SDS,0.02mol/LTris-HCl,NaCl,pH7.2),46°CddH2O3,(Motic,BA200,),30FISH(Moticam2206)MoticFluo1.0,,,(1)[14](%)100%(1)1SBRTable1OperationconditionsofeachrunningstageinSBRRunningphaseRunningtime(d)Averageinfluentammoniaconcentration(mg/L)Averageinfluentammoniaload[kg/(m3·d)]Alkalinity(mg/L)Run-11−6153.60.009300Run-262−8183.40.017500Run-382−96120.10.024700Run-497−118275.20.05515002FISHTable2ProbesusedinFISHProbeTargetpopulationSequence(5′→3′)MarkertypeReferenceUNIV1392ACGGGCGGTGTGTAC5′FITC[15]NSO190CGATCCCCTGCTTTTCTCC5'HEX[16]NIT3CCTGTGCTCCATGCTCCG5′FITC[17]6002012,Vol.39,No.5(2)MPNAOBNOBMPN[8,18]1.2.2:MLSS[19],(2)223NONONOCCC(2),2NOC,mg/L;3NOC,mg/L22.1SBR4,Run-1,Run-2−Run-4,118d1,pH2,0.009kg/(m3·d),DO2mg/L0−40d,8.2%,,0,,;pH,7.327.03,,,,,46d,;61d,100%,80%,,,,Run-262d81d,0.017kg/(m3·d),NaHCO3,pH8.63,,97.3%,0.016kg/(m3·d),pH7.14,68.1%,,,,,pH,DO,Run-382d96d,0.024kg/(m3·d),pH9.02,DO1mg/L,97.3%,0.023kg/(m3·d),pH7.25,76.0%,,Run-497d118d,0.055kg/(m3·d),pH9.08,,77.8%,0.043kg/(m3·d),pH7.17,83.4%,,,;,,AOBNOB[20−22],:601://journals.im.ac.cn/wswxtbcn3.69g/L,,,,4,00.051kg/(kg·d);Run-2−Run-4,0.120.140.16kg/(kg·d),,2.22.2.1AOBNOB:AOBNOB,,AOBNOBMPNAOBNOB,5,NOBAOB;Run-1,AOBNOB,NOB2.0×105CFU/mL1.15×105CFU/mL,AOB4.5×104CFU/mL3.0×105CFU/mLRun-2,NOB9.5×104CFU/mL,AOB1.15×106CFU/mLRun-3,DONOB,AOB,,AOBNOB3Fig.3Variationofsludgeconcentrationduringstart-upandoperationofpartialnitrificationprocess4Fig.4Variationofammoniaremovalsludgeloadingduringstart-upandoperationofpartialnitrificationprocess:603×104CFU/mL,AOB7.5×106CFU/mLRun-4,NOB1.5×104CFU/mL,AOB1.5×107CFU/mL,,AOB,,NOB,DONOB2.2.2AOBNOB:,FISHAOBNOB,AOBNOB6AOB,(1d)AOB,;(61d),AOB,AOB;Run-2(81d),AOB;Run-3(96d)DO,AOB,AOB;Run-4(118d),AOBRun-3,FISHAOBAOB7AOBNOB,5,Run-3AOBNOB,DO,AOBNOBRun-2Run-4,AOB0.016kg/(m3·d)0.043kg/(m3·d);,,0.12kg/(kg·d)6AOBFig.6VariationofAOBdistributioninthesludgeduringstart-upandoperationofpartialnitrificationprocess6042012,Vol.39,No.5