对比分析HRT对ACR乙醇型和丁酸型发酵制氢系统的影响昌盛

36420164ActaScientiaeCircumstantiaeVol．36No．4Apr．2016No．51508539No．2014ZX07405-001SupportedbytheNationalNaturalScienceFoundationofChinaNo．51508539andtheNationalMajorProjectsofWaterPollutionControlandTreatmentNo．2014ZX07405-0011983—*[e-mail]1983—maleassistantprofessorPh．D．*[e-mail]10．13671/j．hjkxxb．2015．0607．2016．HＲTACＲJ．3641266-1272ChangSLiuFLiJZ．2016．ComparisonoftheinfluenceofHＲTonbiohydrogenproductionfromethanol-typefermentationandbutyric-typefermentationinACＲsystemJ．ActaScientiaeCircumstantiae3641266-1272HＲTACＲ1*221．1000122．1500902015-06-042015-08-052015-08-05ACＲpH4．5～5．0、5．5～6．0HＲT．COD=5000mg·L－1HＲT12h4hHＲT=4h30．1L·d－113．5mmol·g－1·d－1HＲTHＲT8h9．3L·d－1．HＲT4～12hACＲ1．5～2．2．HＲT0253-2468201604-1266-07X703AComparisonoftheinfluenceofHＲTonbiohydrogenproductionfromethanol-typefermentationandbutyric-typefermentationinACＲsystemCHANGSheng1*LIUFeng2LIJianzheng21．StateKeyLaboratoryofEnvironmentalCriteriaandＲiskAssessmentChineseＲesearchAcademyofEnvironmentalSciencesBeijing1000122．SchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnologyHarbin150090Ｒeceived4June2015receivedinrevisedform5August2015accepted5August2015AbstractTheinfluenceofHＲTontheperformanceofhydrogenproductionwithethanol-typefermentationandbutyric-typefermentationinanaerobiccontactreactorACＲwascomparedinthispresentstudy．Theethanol-typefermentationandbutyric-typefermentationwasestablishedbycontrolledthepHat4．5～5．0、5．5～6．0respectively．Theresultsshowedthathydrogenproducingmicroflorwasenrichedinethanol-typefermentationastheHＲTwasshortedfrom12hto4hunderconstantinfluentchemicaloxygendemandCOD5000mg·L－1andthehydrogenproductionrateandthespecifichydrogenproductionrateofsludgewaspeakedat30．1L·d－113．5mmol·g－1·d－1underHＲTof4hrespectively．Whereasinthebutyric-typefermentationsystemthebiomassconcentrationwasdecreasedastheHＲTwasshortedwhichinducedthesubstratedegradingefficiencydeclinedandthehydrogenproductionratewasclimaxedatonly9．3L·d－1underHＲTof8h．Thisstudyshowedthatthehydrogenproductioncapabilityofethanol-typefermentationwasadvantagedoverbutyric-typefermentationastheHＲTofACＲrangedfrom4hto12handthehydrogenyieldofethanol-typefermentationwas1．5～2．2timesasthatofbutyric-typefermentation．KeywordsanaerobiccontactreactorACＲhydraulicretentiontimeHＲTfermentativehydrogenproductionethanol-typefermentationbutyric-typefermentation4HＲTACＲ1IntroductionLeeandＲittmann2009Ｒenetal．2007aＲenetal．20062012Ｒenetal．2007a2012Lietal．2007Ｒamachandranetal．2008Panetal．2008Wuetal．2008Panetal．2008Wuetal．1995Ｒenetal．2007Ｒenetal．2007Lietal．2007Xingetal．2006Guoetal．2008Ｒenetal．2007aＲenetal．2007bLietal．2007Lietal．2009Xingetal．2006Lietal．2007Xingetal．2006Guoetal．2008．Ｒenetal．2007aＲenetal．2007bLietal．2009．Showetal．2011Ｒenetal．2010VanandLoganBruce2005Zhangetal．2006．．、．HＲTHＲTWangandWan2009．HＲTShenetal．2009Wuetal．2008Lietal．2010．ACＲHＲT．2Materialsandmethods2．1ACＲ“”ZL200710144460．1200810．8L1．35±1℃．1ACＲFig．1SchematicdiagramofACＲ2．2．C、N、P200～500∶5∶1N、P．、2012．2．3、ACＲ3．58gMLVSS·L－1．pH．COD5000mg·L－1HＲT76213612hpH4．5～5．0、5．5～6．0HＲT12h8h、6h、4hHＲTACＲ．HＲT10d．2．4pH、alkalinity、CODMLSSMLVSSAmericanPublicHealthAssociation1998．-2012．、、VFAsSP-68902015SP-6801T2015．3Ｒesultsandanalysis3．1HＲT3．1．12ACＲHＲT．HＲT．HＲTHＲT．HＲT12h8h、6h、4hACＲ36．4、43．6、56．2、61．6L·d－113．9、20．9、26．5、30．1L·d－1HＲT=12h38．1%HＲT=8h48．1%HＲTHＲT4h～8h47．1%～48．9%2．HＲT12h8h．HＲT12h8hHＲT8h4h．ACＲHＲT=4h．Ｒenetal．2006CSTＲHＲT=4hACＲCSTＲ．2ACＲHＲTFig．2Biogasproductionrateofethanol-typefermentationinACＲsystemunderdifferentHＲT3．1．2HＲT．WuLiHＲTWuetal．2008Lietal．2010．．ACＲHＲT3．3HＲT12h6h814．8mg·L－11643．1mg·L－1HＲT4h1312．1mg·L－1HＲT12h86214HＲTACＲ338．7mg·L－1HＲT8h643．5mg·L－1．pH4．5～5．0HＲTHＲTHＲT4h．pH4．5～5．0pH5．5～6．5、4．0～4．5Ｒenetal．2007aLietal．2009HＲT=12hHＲTHＲT4～12h、HＲT．152．3～185．9mg·L－176．8～134．7mg·L－13．ACＲHＲT12h4h、、．HＲT12h、8h、6h、4h78．2%、87．7%、90．6%、88．5%HＲTHＲTACＲACＲHＲT2．3ACＲHＲTFig．3Solublemetabolitesofethanol-typefermentationinACＲsystemunderdifferentHＲT3．2HＲT3．2．1ACＲHＲT4．ACＲHＲT12h8h9．0L·d－19．3L·d－1HＲT4～8hACＲHＲTHＲT=8h36．8%HＲT=4h24．9%4．ACＲHＲT．ACＲHＲT8h6h25．4L·d－129．0L·d－18．5L·d－17．2L·d－14．HＲTACＲACＲHＲT=8h．4ACＲHＲTFig．4Biogasproductionrateofbutyric-typefermentationinACＲsystemunderdifferentHＲT9621363．2．2ACＲHＲT5．HＲT12h8hHＲT4．HＲT8h4hHＲT=8h1116．9mg·L－1HＲT=4h803．8mg·L－1HＲT=8h211．6mg·L－1HＲT=4h416．9mg·L－15．LeeandＲittmann2009．ACＲHＲT4～8hHＲTHＲT4．HＲTACＲHＲTACＲACＲHＲT8h4．5ACＲHＲTFig．5Solublemetabolitesofbutyric-typefermentationinACＲsystemunderdifferentHＲT3．3HＲTACＲHＲT6．HＲTHＲT．HＲT6～12hHＲTHＲT．HＲT4hACＲHＲT=6h9．6g·L－19．2g·L－16．HＲT8hHＲTHＲTHＲT=4hACＲ3．47g·L－1HＲT6．ACＲHＲT2～5．6HＲTACＲFig．6BiomassconcentrationofACＲsystemunderdifferentHＲTbyethanol-typefermentationandbutyric-typefermentation3．4ACＲHＲT=4hACＲHＲT=8h．ACＲHＲT1．ACＲHＲT12h、8h07214HＲTACＲ94．8%、95%87．5%、86．3%HＲT6h、4h85．6%、72．9%66．0%、60．7%HＲT35HＲT6．HＲTHＲTACＲHＲT．HＲT12h、8h、6h、4hACＲ7．8、10．9、11．4、13．5mmol·g－1·d－16．8、7．1、7．8、8．6mmol·g－1·d－1．HＲT12h、8h、6h、4h1．9、2．2、2．1、1．8mol·mol-glucose－11．3、1．0、1．0、0．5mol·mol-glucose－11．5～2．2．HＲT、、ACＲ．COD5000mg·L－180%ACＲHＲT6h8h．1HＲTACＲTable1Comparisonofchara