反硝化除磷颗粒污泥培养方式的对比实验研究张慧

3　11环境工程学报Vol.3,No.11200911ChineseJournalofEnvironmentalEngineeringNov.2009张　慧　张捍民＊　杨凤林　王新华(,116024)　　(SBAR)。R1//(A/O/A),(DPAOs);R2/(A/O),,A/O/A,DPAOs。,R2,30.4m/h1.022g/cm3,R135.9m/h1.061g/cm3;R1COD、NH+4-N、TNTP86%、98%、82%91%,R286%、99%、74%66%;183d,DPAOs44.7%20.9%。　　　SBAR　DPAOs　X705　　　A　　　1673-9108(2009)11-2061-06ContrastexperimentalstudyonthecultivationmodeofdenitrifyingphosphorusremovalgranulesZhangHui　ZhangHanmin　YangFenglin　WangXinhua(KeyLaboratoryofIndustrialEcologyandEnvironmentalEngineering,MOE,SchoolofEnvironmentalandBiologicalScienceandTechnology,DalianUniversityofTechnology,Dalian116024,China)Abstract　TwosameSBARs(sequencingbatchairliftingreactors)wereusedtostudythecultivationmodeofthedenitrifyingphosphorusremovalgranules.R1wasoperatedundertheA/O/A(anaerobic/oxic/anoxic)modeallalong,enrichingDPAOs(denitrifyingphosphorus-accumulatingorganisms)withthegranulationprocess;whileR2wasoperatedundertheA/O(anaerobic/oxic)modefirstly,thenaddedananoxicphasewhengranulationfinishedinordertoformA/O/AmodealsoandstrengthentheenrichmentofDPAOs.ItwasshownthatR2tookshortertimeforgranulationthanR1,butgranularsettlingvelocityandspecificgravityinR2(30.4m/hand1.022g/cm3)werelowerthanthatofR1(35.9m/hand1.061g/cm3).TheaverageremovalefficienciesofCOD,NH+4-N,TN,TPinR1were86%,98%,82%and91%respectively.Incomparison,thecorrespondingvaluesinR2were86%,99%,74%and66%respectively,whichwerelowerthanthatofR1.Fromtheresultsofday183,itwasindicatedthatDPAOsfractioninR1andR2were44.7%and20.9%.Keywords　denitrifyingphosphorusremoval;aerobicgranules;SBAR;DPAOs:(50878034):2008-11-13;:2008-12-04:(1984～),,,。E-mail:[e-mail]＊,E-mail:[e-mail]　　(denitrifyingphosphorus-accumulatingorganisms,DPAOs)/(A/A),NO-3-N,,。,PHB,“”,50%,30%,15%[1～4]。,、。75%,7%～17%[5],,,--,,,,。[6,7],P/COD;Naohiro[8]A/O/A(//3),FISH。,SBAR(sequencingbatchairliftingreactors)R1R2,R1A/O/A,,DPAOs;R2A/O(/),,A/O/A,DPAOs。,,。,,。1　1.1　SBAR,4L,3.1L;100mm,388mm,H/D3.88;1.55L,50%。,(DO0.2mg/L)(DO1mg/L),JJ-1,120r/min。0.5m3/h,1.77cm/s。、、。(18±3℃)。1。1　SBARFig.1　SchematicdiagramofSBARinstallation1.2　,,、、。MLVSS/MLSS0.53,SVI120mL/g,0.1mm,0.08mm。1.3　,、NH4ClKH2PO4、。NaHCO3NH4Cl3,pH。,:CaCl260mg/L,MgSO4·7H2O25mg/L,FeSO4·7H2O20mg/L,EDTA20mg/L;:FeCl3·6H2O1.5mg/L,H3BO30.15mg/L,CuSO4·5H2O0.03mg/L,KI0.03mg/L,MnCl2·4H2O0.12mg/L,ZnCl20.058mg/L,CoCl2·6H2O0.15mg/L,Na2MoO4·2H2O0.06mg/L。pH,7.5。1.4　(1)NH+4-N、TP、COD、MLSSSVI[9];TN、TOCTNM-1(ShimadzuCorporation,Japan);DO(ModelYSI-55)。(2);(Lasentec,Redmond,USA);(SEM)(KYKY-2800B,Beijing,China);、PHB[10]、Ghan-grekar[11]Jian[12]。(3)DPAOsPAOs/,DPAOs[13],(1):RDPAOs=qanox/qae(1),RDPAOsDPAOsPAOs;qanox(mgTP/gVSS·h);qae(mgTP/gVSS·h)。2　2.1　,。2。20071118,150mL。,,90min。4d,R1R2MLSS6934mg/L6112mg/L,SVI115.24mL/g117.42mL/g,。190min30min,。206211　:,MLSS。,,F/M,,MLSS5000mg/L,SVI。27d,R2,6d,R1。H/D(3.88),[14]。R1R2SVI56.13mL/g61.22mL/g,8min。50d,。60d4℃,33d,。3MLSS,。,SVI,130d,SVI20mL/g,R2SVIR1,。,,MLSS。170d,　　　1.5min0.6min,,,R2,,MLSS4000mg/L。125dSEM,4。4　125dR1(a,b)、R2(c,d)Fig.4　SEMimaginesofgranularsurfaceinR1andR2atday125SEM:R1R2,。,[15]。,R1,;R2,R2。134d1。,,R1,R2,2;R1,。1　Table1　Comparisonofphysicalcharacteristicsofgranules(m/h)(mm)(g/cm3)(%)R135.91.451.06198～100R230.41.241.02298～1002.2　。,DPAOs。R1R220633,。2。4,120min,R1R2。139d,R2。214d,180min,150min,R1。R2,,,。2　Table2　Variationsofinfluentconcentrationsandoperationalparameters(d)C/N/PR1(min)R2(min)(min)Ⅰ1～7500/50/5120/360/120120/480600Ⅱ8～26500/50/10120/360/120120/480600Ⅲ27～47400/50/10120/180/120120/300420Ⅳ48～138400/40/10120/180/120120/300420ⅤⅥ139～213214～241400/40/10400/40/10120/180/150120/180/150120/240/90120/180/150450450Ⅰ1,。C/N/P100∶10∶1,,ⅡTP10mg/L,19d。R1,:PHBTP,DPAOs。:R1180min,R2300min,COD400mg/L。[16],。27d,Ⅲ。ⅢTP,:NO-3-N4min,,,。,NH+4-N40mg/LNO-3-N。Ⅳ91d,。33dC、NR1P,R2TP。DPAOs。。ⅤR1150min,R2240min,90min。DPAOs,R1103d,R275d。ⅥR2150min,180min,。5R1、R2C、NP。,C、NP30.48～0.74、0.063～0.0760.011～0.018kg/(m3·d)。C,R1COD59.72mg/L85.90%,R257.18mg/L86.48%。C,C95%。R1、R2NH+4-N1mg/L,98.46%99.17%。R2NH+4-NR1,R2。R1,TNR2,N,,R1TN82%,R2139d,TN,74%,R1。:(1)R1,DO,;(2)R1A/O/A,。TP。R1TP1.74mg/L91.13%,R24.55mg/L65.56%。R2,NO-3-N,,PAOs,。139d,R2TP,139d46.41%56.01%,R1。A/O/ANO-3-N,,TP。NO-3-NNH+4-N,R15.78mg/L16.46mg/L,R28.45mg/L19.68mg/L。NO-3-NPHB,TP。2.3　175d,PPHB,6。R1R22PAOs206411　:5　C、NPFig.5　ComparisonofC,NandPremovalinR1andR2[17],P,PHB,P6.2%3.7%,R2R160%,R1。R1,PR2,PAOs。2.4　DPAOs183d7。R130min3.126mgTP/(gVSS·h)1.398mgTP/(gVSS·h),R26　PPHBFig.6　ProfilesofPcontentofgranularsludgeandPHBinacycletimeatday1751.608mgTP/(gVSS·h)0.336mgTP/(gVSS·h)。,[8]。,R1DPAOs44.7%,R220.9%,R147%,DPAOs,A/O/A,DPAOs。7　30minFig.7　ComparisonofSPUR30ofanoxicandaerobicphaseofgranulesatday183206533　　(1)R2(A/O※A/O/A)R1(A/O/A),;134d,R230.4m/h1.022g/cm3,R135.9m/h1.061g/cm3。(2)A/O/AA/O※A/O/A,R1COD、NH+4-N、TNTP86%、98%、82%91%,R286%、99%、74%66%。(3)A/O/ADPAOs,A/O/A183d,DPAOs44.7%,A/O※A/O/A。[1]KubaT.,vanLoosdrechtM.C.M.,HeijnenJ.J.Phos-phorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrifica-tioninatwo-sludgesystem.WaterRes.,1996,30(7):1702～1710[2]KubaT.,vanLoosdrechtM.C.M.,HeijnenJ.J