反硝化除磷脱氮系统中DPB的驯化富集培养

31520105ENVIRONMENTALSCIENCEVol.31No.5May2010DPB122311.5106552.1000223.150090DPBDPB/DPBDPB.15d/A/OPAOs//A/O/A25dDPBPAOsDPB/19dDPB.DPBX703.1A0250-3301201005-1252-052009-06-112009-09-09zx-200712-212008ZX07211-0042006DFB915501980～[e-mail]entofDenitrifyingPhosphorusRemovalBacteriaDPBinDenitrifyingBiologicalNutrientRemovalProcessHUANGRong-xin12ZHANGJie23CHENJian-yu11.SouthChinaInstituteofEnvironmentalScienceMinistryofEnvironmentalProtectionGuangzhou510655China2.SchoolofEnvironmentalandEnergyEngineeringBeijingUniversityofTechnologyBeijing100022China3.SchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnologyHarbin150090ChinaAbstractThecultivationofthedenitrifyingphosphorusremovalbacteriaDPBinbiologicalnutrientremovalsystemwilldirectlyaffecttheremovalefficiencyofthenutrientsinwastewatersuchasnitrogenandphosphorusandthestabilityoftheprocess.ForthisreasonanewBNRprocesswhichiscalleddouble-sludgedenitrifying-nitrogenandphosphorusremovalprocesswasdesigned.Inordertocreateagoodanaerobic/anoxicalternatingenvironmentforDPBgradualtransitionoftrainingmethodswereusedtomakeDPBnaturalselectionandthenfinallyscreenedthedominatedDPBbacteria.Theresultsindicatedthattheanaerobic/aerobicA/OoperationmodecaninduceinductionofPAOsrapidlyby15daysintermittentaeration.TheproportionofDPB/PAOsinthereactorwasaggrandizedinthesecondphaseabout25days'inducementbyreducingtheaerationtimeinaerobicphasegraduallyandaddingnitrateinanoxicphasewhichiscalledanaerobic/aerobic/anoxicA/O/Amodefinallythestrictlyalternatingenvironmentalanaerobic/anoxicconditionsforDPBwereusedtoinduceandenrichthetargetDPBbacteriafor19daysbyagradualtransitionofenrichmentculture.ThesuccessfuldomesticationandcultivationofDPBprovidesanewmethodtoremovenitrogenandphosphorusefficientlyandsteadilyinmunicipalwastewater.KeywordsdenitrifyingphosphorusremovalbacteriaDPBelectronacceptorbiologicalnutrientremovalphosphorusaccumulatingorganismsPAOs1.PAOspolyhydroxyalkanoatesPHAPAOsPHA、PAOs“”23.4～7PAOs2denitrifyingphosphorusremovalbacteriaDPB5DPBaerobicPAOs.PAOsDPBPAOsA/O、A2/OUCTDPB8～12.13～15.DPBDPB.DPB.DPB/DPB3.11.116～18DPBPHB2CODDPBPHB.20minDO5～6mg/L..1.1.2.1.1Fig.1Layoutoftwo-sludgedenitrifyingBNRprocess1Table1AnalysisparametersandmethodsofthewaterqualityCODTPPO3－4-PNO－2-NN-1--MLSSpHpH22.1A/OPAOsDPBA/OA/O2h+4hPAOsPAOs.25d3521314L/h8～15mg/LCOD200～250mg/L2h4h、、SRT=15d2.2A/O4hFig.2TPchangesafter4hintermittentaerationundertheA/Ooperation225d15d6mg/LA/O18d20mg/LTP1mg/L..PAOsDPB.2.2A/O/APAOsDPBPAOsDPBPAOs//A/O/AA/OPAOsA/O/APAOsDPB.DPB.6h1.5mL/min100mg/LSRT10d23.2Table2Parametercontrolofoperationmodeandwaterquality/d/mg·L－1/hCODTP1～5230～2708～152.04.06～10230～2708～151.54.511～15180～2208～151.05.016～20130～1708～150.55.521～2580～1208～1506.03A/O/AFig.3LabscaleequipmentofA/O/AdenitrifyinginducementDPBDPB.1、、4.4A/O/AFig.4TPchangecurvesundertheA/O/Adenitrifyinginducement4225dA/O/AO.45215DPB△TP=5.88mg/L△TP=11.94mg/LPAOs———.DPB5d.DPB3.2.3A/ADPB2DPBDPB.CODCODDPBCOD.COD.COD/.DPB.1、5.58～14mg/L6.5635A/AFig.5TPchangecurvesundertheA/Adenitrifyinginducement6Fig.6AssimilatingrateofTPonanoxicphaseafteranaerobicphaseA/OPAOsDPBPAOsDPB/.6395%.DPB/..31/A/O15dPAOs.22//A/O/A25dPAOs552131DPB.3DPB/19dDPB.1.M.1998.2.ORPJ.200425454-58.3.C/NJ.2005253367-371.4.J.2005214243-249.5.J.200525S1115-118.6.J.200622S52-57.7.J.200622335-39.8MeinholdJArnoldEIsaacsS.EffectofnitriteonanoxicphosphateuptakeinbiologicalphosphorusremovalactivatedsludgeJ.WaterRes19993381871-1883.9NaohiroKJuhyunKSatoshiTetal.Anaerobic/oxic/anoxicgranularsludgeprocessasaneffectivenutrientremovalprocessutilizingdenitrifyingpolyphosphate-accumulatingorganismsJ.WaterRes2006402303-2310.10MerzoukiMBernetN.BiologicaldenitrifyingphosphorusremovalinSBReffectofaddednitrateconcentrationandsludgeretentionTimeJ.WaterSciTechnol2001433191-194.11HuJYOngSLNgWJetal.AnewmethodforcharacterizingdenitrifyingphosphorusremovalbacteriabyusingthreedifferenttypesofelectronacceptorsJ.WaterRes2003373463-3471.12MeinholdJCarlosDMFDaiggerTGetal.CharacterizationofthedenitrifyingfractionofphosphateaccumulatingorganismsinbiologicalphosphateremovalJ.WaterSciTechnol199939131-42.13FilipeCDMDaiggerGTGradyCPL.pHasaKeyFactorinthecompetitionbetweenglycogen-accumulatingorganismsandphosphorus-accumulatingorganismsJ.WaterEnvironRes2001732223-232.14FilipeCDMDaiggerGTGradyCPL.EffectofpHontheratesofaerobicmetabolismofphosphate-accumulatingandglycogen-accumulatingorganismsJ.WaterEnvironRes2001732213-222.15JeonCOLeeDSLeeMWetal.Enhancedbiologicalphosphorusremovalinanaerobic-aerobicsequencingbatchreactoreffectofpHJ.WaterEnvironRes2001733301-306.16FilipeCDMDaiggerGTGradyCPL.AmetabolicmodelofacetateuptakeunderanaerobicconditionsbyglycogenaccumulatingorganismsstoichiometrykineticsandtheeffectofpHJ.BiotechnolBioeng200176117-31.17LiXKHuangRXBaoLLetal.Simultaneousphosphorusandnitrogenremovalinacontinuous-flowtwo-sludgesystemJ.JEnvironSci200618152-57.18HuangRXZhangJMaLetal.PositiveroleofnitriteaselectronacceptoronanoxicdenitrifyingphosphorusremovalprocessJ.ChineseSciBull200