反硝化细菌研究进展方晶晶

33430074、、、。X172Adoi10.3969/j.issn.1003-6504.2010.6E.0541003-6504(2010)6E-0206-05TheAdvanceofStudyonDenitrifyingBacteriaFangJing-jing,MaChuan-ming,LiuCun-fuSchoolofEnvironmentalStudies,ChinaUniversityofGeosciences,Wuhan430074ChinaAbstractTheadvanceofstudyonthekindsofdenitrifyingbacteria,morphologicalcharacteristics,applicationanddenitrifyingfactorsathomeandabroadisbrieflyreviewed,andthefutureutilizationisdiscussedinthispaper.Keywordsdenitrifyingbacteria;denitrification;denitrificationfactors2010-05-142010－06－23(40802057)[e-mail]。。、、、。。、。。2001Sigman[1]2006JulieGrangerSigman[2]2007JohnKarlBhlke[3]N2O。1、[4]50130。(Pseudomonaceae)、(Al－caligenes)(Neisseriaceae)、(Nitrobacteraceae)、(Rhodospirillaceae)、(Bacillaceae)、(Cytopha－gaceae)、(Spirillaceaee)、(Rhizobiaceae)、(Halobacteriaceae)。[4](bacillus)、(Alcaligenes)、(Paracoccus)、(Klebsiella)、(Ochrobactrum)、(Zoogloea)、(Pseudomonas)、(HyphomicrobiumX)、(Agrobacterium)、(Eubacterium)、(Propionibacteri－um)、(Blastobacter)、(Halobacteri－um)(Rhizobium)。19904(Micrococcusdenitrificans)、(Pseudomonasdenitrificans)、(Pseudomonasfluorescens)、(Chromobacteriumdenitrificans)(Chro－mobacteriumviolaceum)。EnvironmentalScience&Technology336E20106Vol.33No.6EJune20106E,[5](Arthrobacter)、(Achro－mobacter)、(Acinetobacter)、(Alcaligeties)、(Bacillus)、(Brevibacillus)、(Cellulosimicrobium)、(Chromobacterium)、(Halomonas)、(Halobacillus)、(Micrococcus)、(Microbacterium)、(Spirillum)、(Oceanimonas)、(Paracoccus)、(Planococcus)、(Planomicrobium)、(Stenotrophomonas)、(Pseu－domonas)(Sphingobacterium)。BacillusPseu－domonas[5]。[6]。2(Micrococcus)0.5～3.5μm[5]。、、[5]。。。。。。。。5％NaCl。(Schleifer&KloosJClinMicrobiol337～3381975)。25～37℃。。Pseudomonas。0.5～1.0μm*1.5～4μm。。。-β-。。。。。。。。H2CO。。。、。Chromobacterium0.6～0.9μm*1.5～3.5μm。。。。1～4。。25℃、pH7.8pH4.5。6％NaCl。。、、。、、、、。(Kovacs's)。。。V-P。。。、。、、。ONPG、DNA、。KCN。(100μg/mg)0/129。。。33.1[8]、、。A/OA/O-(Anoxic/Oxic)。A/O2,,,,。,,,,,,[6]O/AO/A-(Ox－ic/AnoxicA/O[6]。)A/O85%O/A207333.2[21]D2、。3.3[22]Agrobacteriumsp.、、[22]。44.1[23]、3X31,(C/N)X31。。。11.86mg/(g·h)。[24]、。4～5GVSS/L0.486gNO3-N/gVSS·d。。[7-10]。(CO2HCO3-)[11-15]-。1,2,[11,16-18]。。[4]、、、、1[4]。()。NapANapB。NapAnapA,MGD()[4Fe-4S]。NapBC552。(),。3α()。αnarG。βnarH4[4Fe-4S]。γnarI2b。N2OpHN2O。bc,2,NORBb,NORCcnorCB。。NONONO。NON2O。(cd1)nirs。2(His-17,His-69)c,(Tyr-25)(His-200)d1。c,d1O2。Cu。nirk。N2。pH。6Cu,2,CuA,CuZ。Cytc554。1、、、、2086E。4.2[23]X31(C/N)。C/N＞5,90%。5～6,。C/N1～14,4～10h[23]。[20]A11099%。。4.330℃5℃40℃[24]。Blowes5～19℃,NO3-＜8℃,[28]。Volokita400g,24℃4,14℃19℃,N。,19℃24℃60%,,30℃14℃2[28]。Volokita[26]400g,25℃,:25～32℃14℃3。Volokita。4.4pH[29]Pseudomonaschloritidis－mutanspHpH。pH7.5～9.2。pHpH7.37.3N2O[24]。Simek[28]pH＞7N2N2O。[30]pH。pH[31]。[32]pHpHpH7.5pH7.0～8.0。pH[27]。4.5DODO3mg/LDOPseudomonassp.DO4mg/LDO2mg/LCitrobacterdiversusDO5mg/L[33]。[25]A15mg/L99%。5、、1、。2[5]。3[5]。[][1]SigmanDM,KLCasciotti,MAnfreani.Abacterialmethodforthenitrogenisotopicanalysisofnitrateinsea－waterandfreshwater[J].AnalyticalChemistry,2001,73(17):4145-4153.[2]JulieGranger,DanielMSigman.Amethodfornitritere－movalinnitrateNandoisotopeanalyses[J].LimnologyandOceanography:Methods4,2006,205-212.[3]JohnKarlBo1hlke,RichardLSmith,JanetEHannon.Iso－topicanalysisofNandOinnitriteandnitratebysequentialselectivebacterialreductiontoN2O[J].AnalyticalChem－istry,2007,79:5888-5895.[4],,,.[J].,2009,32(12)98-102.XiaoJing-jingGuoPingHuoWeietal.Applicationof,20933denitrifyingmicrobestowastewaterdenitrification[J].Envi－ronmentalScienceandTechnology,2009,32(12)98-102.(inChinese)[5],,.[J].20099(17)5057-5064.WangXiao-shan,LiuJie,YuJian-sheng.ResearchprogressinmarinenitrogencyclingBacteria[J].Geoscience,20099(17)5057-5064.(inChinese)[6]ZumftWG.Cellbiologyandmolecularbasisofdenitrifica－tion[J].MicrobiologyandMolecularBiologyReviews199761533-616.[7].[M].,1990,(4)29.LuoXing-yi.TheSignificanceofDenitrifyingBacteriainNature[M].BiologyTeaching,1990,(4)29.(inChinese)[8],.[J].,2005,25(2):88-92.RenYan-li,JingYuan-xiao.Theroleofdenitrificationbac－teriainwastewatertreatment[J].JournalofMicrobiology,2005,25(2):88-92.(inChinese)[9]RorbertsonLA,KuenenJG..Aerobicdenitrification-oldwineinnewbottles[J].AntonicvanLeeuwenhoek,1984,50:525-544.[10]RorbertsonLA,KuenenJG.Aerobicdenitrification:acon－troversyrevived[J].ArchivesofMicrobiology,1984,139:351-354[11]PaiSL,ChongNM,ChenCH.Potentialapplicationsofaerobicdenitrifyingbacteriaasbioagentsinwastewatertreatment[J].BioresourceTechnology,1999,68(2):179-185.[12]ZartD.HighrateofaerobicnitrificationanddenitrificationbyNitrosomonaseutropha-growninafermentorwithcompletebiomassretentioninthepresenceofgaseousNO2orNO[J].ArchivesofMicrobiology,1998,169(4):282-286.[13]BatchelorB,LawrenceAW.Autotrophicdenitrificationus－ingelementsulfur[J].WaterPollutionControlFed,1978,50:1986-3001.[14]BaalsruudK,BaalsruudKS.StudiesonThiobacillusdeni－trificans[J].ArchivfurMicrobiologle,1954,20:34-62.[15]BachelorB,LawrenceAW.Akineticmodelforautotroph－icdenitrificationusingelementalsulfur[J].WaterResearch,1978,12(1):75-84.[16]BachelorB,LawrenceAW.Stoichiometryofautotrophicdenitrificationusingelementalsulfur[C].InChemist