复合型微生物絮凝剂产生菌的培养及对炼化废水处理的研究

:,,1983,,#3(No.06A0302)3#((),266555)5,SHD21SHD252,SHD21SHD25:48h,pH7,160r/min+(NH4)2SO4(11),COD93.45%53.42%20.98%,,Cultivationofcompoundbioflocculant2producingbacteriumanditsapplicationinrefinerywastewatertreatmentLIZheng,WUWeilin,ZHANGYunbo,LIUQiyou,ZHAODongfeng.(CollegeofChemistryandChemicalEngi2neering,ChinaUniversityofPetroleum(EastChina),QingdaoShandong266555)Abstract:Fivebioflocculant2producingstrainswerescreenedfromtheactivatedsludgeofrefiningandchemicalplant;thefivestrainswerenamedSHD21toSHD25indueorder.Aftertwoarbitrarystrainsweremixedandcultivat2ed,itwasfoundthatthecompoundbioflocculant2producingstrainsSHD21andSHD25hadthebesttreatmenteffectforrefinerywastewater.Theoptimumcultureconditionsoftheselectedstrainswereascertainedthroughsinglefactorexperiments.Theresultsshowedthattheoptimumconditionswerecultureperiod48hours,initialpH7,rotationspeed160r/min,glucoseascarbonsource,peptone+ammoniumsulfate(massratio11)asnitrogensource.Undersuchoptimizedconditions,theturbideliminatingratewasupto93.45%,theremovalrateofpetroleumandCODcanreachto53.42%and20.98%,respectively.Thebioflocculantwasmuchbetterthaninorganicflocculants.Keywords:compound;bioflocculantproducingbacterium;culturalcondition;refinerywastewater(MBF)[1]MBF,MBF,,[2],(CMF)MBF[3],,MBF[4,5]5MBF,2,,,CMF,CMF11.11.1.11.1.2,:7581NTU,COD830845mg/L,140150mg/L1.1.3:5g/L;10g/L;NaCl5.0g/L;pH7.47.6:20g/L;(NH4)2SO40.2g/L;0.2g/L;KH2PO42g/L;K2HPO454733320113©1994-2012ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:20g/L;K2HPO412g/L;KH2PO411g/L;Na2SO42g/L;KCl2g/L;MgSO47H2O4g/L;pH,ABC3,3113Table1Nitrogensourceconcentrationintheenrichmentmedium/(gL-1)/(gL-1)/(gL-1)A0.051.050B00.5100C001001.1.4721UV2100,DBJ2623,WMXCOD,LP2000211.21.2.12gA,28160r/min32h,20%(,)B32h,20%C32h310%30mL,28160r/min24h,,,1.2.2,4g/L,,,,,MBF1.2.3,500mL300mL,MBF,,200r/min2min,20r/min6min,10min,,COD,,1.2.4MBF,11,1.2.5,pH1.2.6[6];(GB/T164881996);COD[7]3,22.1,121205MBF,SHD21SHD25,5225Table2Theflocculationactivityof5bioflocculantproducingstrains/%/%COD/%SHD2175.3115.2143.15SHD2250.2925.7519.34SHD2350.2129.2017.80SHD2460.0031.1219.73SHD2559.3928.3018.492.2MBF,33Table3Theflocculationactivityofcompoundbioflocculantproducingbacterium/%/%COD/%SHD21+SHD2588.3446.6114.29SHD22+SHD2575.3831.5413.55SHD23+SHD2578.8842.7614.10SHD24+SHD2588.2842.7612.72SHD21+SHD2277.4838.5219.87SHD21+SHD2378.1339.6511.41SHD21+SHD2486.1439.9112.05SHD22+SHD2378.4839.4411.02SHD22+SHD2488.4540.9813.53SHD23+SHD2488.0841.4415.6557©1994-2012ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(Bacillussp.),2,,SHD21SHD252.328160r/minpH7,122436486072h,,,11Fig.1Effectofculturetimeonflocculationactivity1,12h,24h,48hCOD,,CMF,,[8,9],,,,CMF,,48h2.4pH1mol/LHClNaOHpH,pH45678910,,28160r/min48h,,pH,22,pH7,,COD,CMF,,pH72.528pH7,2pHFig.2EffectofpHonflocculationactivity80100120140160180200r/min48h,,33Fig.3Effectofrotationspeedonflocculationactivity3,120r/min,160r/min,COD88.00%67.10%25.20%,,160r/min,;CMF,,CMF,160r/min2.6,28160r/minpH748h,,,44Fig.4Effectofcarbonsourceonflocculationactivity6733320113©1994-2012ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.()(NaNO3(NH4)2SO4)(++NaNO3+(NH4)2SO4,11)(NH4)2SO4,28160r/minpH748h,,,55Fig.5Effectofnitrogensourceonflocculationactivity5,,,+(NH4)2SO4,COD94.11%67.17%26.24%,+(NH4)2SO42.828160r/minpH720g/L0.5g/L(+(NH4)2SO4,11)48hCMFCMF((PAC)(PAM)),CMF,6,CMFCOD93.45%53.42%20.98%6,3,CMFCODCMF,,6Fig.6Comparisontheflocculationactivityofdifferentflocculants3(1)5MBF,SHD21SHD25,(2):48h,pH7,160r/min,,+(NH4)2SO4(11)(3),CMFCOD93.45%53.42%20.98%,CMF,:[1],,,.[J].,2007,29(10):7352739.[2],,.[J].,2006,22(1):36239.[3]LIANBin,CHENYe,ZHAOJin,etal.MicrobialflocculationbyBacillusmucilaginosus:applicationsandmechanisms[J].BioresourceTechnology,2008,99(11):482524831.[4]GONGWenxin,WANGShuguang,SUNXuefei,etal.BioflocculantproductionbycultureofSerratiaficariaanditsapplicationinwastewatertreatment[J].BioresourceTechnology,2008,99(11):466824674.[5].[J].,2007(1):9.[6].[M].4.:,2002.[7].COD[J].,2002,12(3):32233.[8]PAVONIJL,TENNEYMW,ECHELBERGERWF.Bacteri2alexocellularpolymersandbiologicalflocculation[J].JournalWaterPollutionControlFederation,1972,44(3):4142431.[9],.[J].,2001,24(2):12215.:(:2010208230)77©1994-2012ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.