复合有益菌剂对工厂化大菱鲆育苗水净化效果研究李秋芬

:1000-0615(2006)06-0852-05:2005-03-16:(30200209);“863”(2001AA622070);“863”(2001AA622070):(1969-),,,,。Tel:[phone],E-mail:[e-mail]··李秋芬,　张　艳,　王印庚(,　266071):;;;;:S963.21+1　　　:AStudyonpurifyingeffectofmulti-strainprobioticsproductonwaterofintensiveturbotbreedingLIQiu-fen,ZHANGYan,WANGYing-geng(YellowSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Qingdao　266071,China)Abstract:Onthebasisofpurifyingeffectofsingle-strainprobioticbacteriaonwaterofintensiveturbotbreeding,thepurifyingeffectofmulti-strainbacteriaproductwasstudied.Theresultsofexperimentsshowedthatthefreemulti-strainmixedbacteriahadbetterpurifyingeffectonturbotbreedingwaterthanthatofanysingle-strainbacteria.thisphenomenoncanbeexplainedwiththeprincipleof“bio-pump”.Theimmobilizedmulti-strainprobioticsproduct,bio-film,alsoshowedexcellentpurifyingeffectonwaterofturbotbreeding.TheremovalratesofCODandammoniain5dayswereupto73.1%and80.0%,respectively.Theconcentrationofnitritekeptlow,whichindicatedmostammoniahadbeentransformedintonitrateandaccumulationofharmfulintermediateproduct-nitritewasdecreased.Thispapersuppliedtheoreticalandtechnicalfoundationforsavingseawaterthroughbio-purificationofwastewaterfrommarinefishculture.Keywords:probiotics;probioticsproduct;waterpurification;Scophthalmusmaximus;marinefishculture　　、、[1],,、,[2-4],,。,。、、、,[5-9]。:、。、。[10];、、、、,[11]。Lekang[12],;[13];[14]。　　,、、、、,,,。　306200612　　　　　　　　　　　　　　JOURNALOFFISHERIESOFCHINA　　　　　　　　　　　Vol.30,No.6　Dec.,2006　,、,。1　1.1　　　Lt7222(Subtilussp.)、A3(Nitrosomonassp.)、Y1(Halomonassp.)X3(Rhodococcussp.),、、,[15]、、,()。,。。1.2　　　游离混合菌剂净化育苗废水　　20L,10L,25d50,1.5～2.5cm,1.0g。5,、、、,2216E。,,8.7,,。15℃,。　　(Lt7222、A3、Y1、X3),OD600nm,,109cells·mL-1,50mL,COD、、,[16],NH4+-N,NO2--NNO3--N;COD。,。　　固定化生物膜法净化育苗废水效果试验　　:,28℃,130r·min-11,10%,10L。　　:():0.5%,0.3%,0.5%,0.03%,0.03%,,pH8.0。,10%,20℃(DO4mg·L-1)。,2pH,NaOH8.0。。。　　:,。2m3,。,。。　　:,1～2,30min,,10m3,COD3。,3COD。。2　2.1　　　有机物降解菌Lt7222对育苗水体的单株净化效果　　,COD,,,,COD。5d2.78mg·L-19.05mg·L-122.5%。COD,5d,12.73mg·L-14.77mg·L-1,COD60.8%(1)。,Lt7222。1　Lt7222Fig.1　Purifyingeffectoforganicpollutant-degradingbacteriaLt7222onrearingwaterofturbot　　氨氧化菌A3的单株净化效果　　2,,A30.34mg·L-10.21mg·L-1,44.1%,0.328mg·L-10.343mg·L-1,(P0.048);0.012mg·L-10.027mg·L-1,0.012mg·L-10.019mg·L-1,,,(P0.027)。8536　　　　　　　　　　:0.50mg·L-10.38mg·L-1,0.48mg·L-10.41mg·L-1,,(P0.72)。,A3。2　A3Fig.2　PurifyingeffectofAmonia-oxidizingbacteriaA3onrearingwaterofturbot　　亚硝化菌Y1的单株净化效果　　3,,Y1,,0.12mg·L-10.44mg·L-1,0.14mg·L-10.24mg·L-1;,,(P0.074);,0.89mg·L-10.65mg·L-1;,,,60.73mg·L-10.79mg·L-1,(P0.5613)。3　Y1Fig.3　PurifyingeffectofnitrificatingbacteriaY1onrearingwaterofturbot　　硝化细菌X3的单株净化效果　　,。,0.12mg·L-10.34mg·L-1,,,。　　,,,0.168mg·L-10.143mg·L-1。0.009mg·L-10.029mg·L-1,,P0.05,。0.89mg·L-10.75mg·L-1,、,,,0.83mg·L-10.94mg·L-1,。4　X3Fig.4　PurifyingeffectofnitrificatingbacteriaX3onrearingwaterofturbot　　复合菌剂对大菱鲆育苗水体的净化效果　　5,COD,5d8.65mg·L-13.05mg·L-1,64.4%;3.78mg·L-18.05mg·L-1(5-a)。,。0.37mg·L-10.14mg·L-1,,67.3%,、。,;,,0.016mg·L-10.052mg·L-1;。0.360mg·L-10.390mg·L-1,,(5-b)。,,,,。2.2　　　2,。6-a,COD,5d5.82mg·L-11.57mg·L-1,73.1%;3.72mg·L-13.05mg·L-1(6-a)。,COD,854　　　　　　　　　　　　　　　　　　　　　　30。　　,0.300mg·L-10.060mg·L-1,80%;,,0.016mg·L-10.052mg·L-1;0.360mg·L-10.390mg·L-1,,(6-b)。5　Fig.5　Purifyingeffectofmixbacteriaonwastewaterfromturbotrearing6　Fig.6　Purifyingeffectofimmobilizedmixbacteriaonwastewaterfromturbotrearing3　　　,,COD68.4%～73.1%,Lt722260.8%,80%,A325.3%Y177.4%,。,,,,,。,,,,,。。,,、,[17,18]。,,,,,。,,,。　　,。,,8556　　　　　　　　　　:,。[10],、,、。,,[19],,,、、、,,,40～50,,[20]。　　、、,,,,,。:[1]　,　,,.“+”[J].,2002,23(4):1-7.[2]　ShiCY,WangYG,YangSHL,etal.Thefirstreportofanirodovirus-likeagentinfectioninfarmedturbot,Scophthalmusmaximus,inChina[J].Aquac,2005,236:11-25.[3]　　,,,.[J].,2004,(3):83-89.[4]　　,,,.[J].,2004,22(2):193-197.[5]　,,,.[J].,1997,27(2):191-194.[6]　BengmarkS.Ecologicalcontrolofthegastrointestinaltract.Theroleofprobioticflora[J].Gut,1998,42:2-7.[7]　GarriquesD,ArevaloG.AnevaluationoftheproductionanduseofalivebacterialisolatetomanipulatethemicrobialflorainthecommercialproductionofPenueusvannameipostlarvaeinEcuador[J].Aquaculture,1995,295:53-59.[8]　GramL,MelchiosenJ,SpanggaardB,etal.InhibitionofVibrioanguillarambyPserdomonasfluorescensAH2-apossibleprobiotictreatmentoffish[J].ApplEnvironMicrobiol,1999,65:969-973.[9]　LaurantV,GeertR,SorgeloosP,etal.Probioticbacteriaasbiologicalcontrolagentsinaquaculture[J].MicrobiologyandMolscularBiologyReviews,2000,64(4):655-671.[10],　,,.[J].,1999,23(2):156-161.[11]　,,.3[J].,2003,10(1):65-68.[12]LekangOI,Kkeppe,H.Efficiencyofnitrificationintricklingfiltersusingdifferentfiltermedia[J].AquaculturalEngineering,2000,21:181-199.[13]　,,　,.[J].,2001,2(1):90-92.[14].[J].,1991,18(2):58-60.[15],,,.[J].,1999,8(1):156-161.[16].[S].:,1991.[17]　,,,.[J].,1999,8(1):156-161[18],.[J].,2000,20(1):68-73.[19],,,.[J].,2004,28(8):5-8.[20],.[J].,2003,8:19-21.856　　　　　　　　　　　　　　　　　　　　　　30