富含聚磷菌的好氧颗粒污泥的培养与特性由阳

1,1,2,3,1,3＊(1.,　150090;2.AdvancedWastewaterManagementCentre,TheUniversityofQueensland,Brisbane　4072,Australia;3.,　100022):SBR,,,2,.,.,,,,,(2mm),(、、、、).,.70%.,,COD95%,100%.:;;;;SBR:X703　:A　:0250-3301(2008)08-2242-07:2007-08-16;:2007-11-07:-(50628808);“”(2006BAC19B03,Z0005186040421):(1979～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]risticofAerobicGranularSludgeEnrichedbyPhosphorusAccumulatingOrganismsYOUYang1,PENGYi1,YUANZhi-guo2,LIXi-yao3,PENGYong-zhen1,3(1.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China;2.AdvancedWastewaterManagementCentre,TheUniversityofQueensland,Brisbane4072,Australia;3.SchoolofEnvironmentalandEnergyEngineering,BeijingUniversityofTechnology,Beijing100022,China)Abstract:Thisresearchfocusedontheenrichmentofphosphorusaccumulatingorganisms(PAO)andtheformationofgranularsludgesimultaneously.AfterfedwithflocculentsludgetheSBRwasrunfortwomonthsforthecultivationofPAO.ThenthegranularsludgeenrichedbyPAOwasfound.Afterthatacetatewasusedinsteadofpropionatetoinhibittheglycogenaccumulatingorganisms(GAO).TheexperimenttestifiedthatacetatewasbeneficialtothegrowthofthePAOgranules.Thesystemcouldreleaseandtakeupmorephosphoruswhenitwasfedbyacetate.Moreover,whenthesizeofthegranulesbecamebigger,theperformanceindexesofthegranules,forexamplethesettlingvelocity,OUR,density,aquiferousrateandintegralratewerealsoimproved.Ontheotherhand,theamountofPAOwasfoundtobecomemoreandmoreinthisprocessbythesystemperformanceevaluationandFISHanalysis.Asaresult,theratioofPAOcouldreach70%ofthetotalbacteria.TheaerobicgranularsludgeenrichedbyPAOshowedverygoodcapabilityofCODandphosphorusremoval.TheCODremovalefficiencycouldreachabout95%andphosphorusremovalefficiencycouldreachalmost100%.Keywords:phosphorusaccumulatingorganisms;aerobicgranularsludge;enhancedbiologicalphosphorusremovalsystem;enrichment;SBR　　,,、SVI、、,[1,2].(auto-immobilizationself-immobilization).1980Lettinga[3](UASB).,(、、)[4～12].、、、、COD,,.Dulekgurgen[13],COD.Lin[14]PCOD,.Liu[15]PCOD,PCOD110010100,1.9%9.3%().(EBPR),29820088　　　　　　ENVIRONMENTALSCIENCEVol.29,No.8Aug.,2008DOI:10.13227/j.hjkx.2008.08.024(PAO),.PAO、COD.PAO,,PAO,PAO.1　1.1　SBRSBR,70cm,20cm,14L,10L,,.HA-L.、、、、.10cm,,,.,.,;20℃,(20±2)℃.1.1.　2.　3.　4.SBR　5.6.　7.　8.　9.　10.1　Fig.1　SchematicexperimentalequipmentsSBR4,6h,2min,2.5h,3h,3min25min.7L,7L,710.8.5h.,1L,10d.1.2　Adrian[16].1,,;.COD800mgL,(PO3-4-P)53.3mgL,CODPO3-4-P15.1　Table1　Compositionofthefeedg·L-1NH4Clg·L-10.1065FeCl3·6H2O1.5MgSO4·7H2Og·L-10.171H3BO30.15CaCl2·2H2Og·L-10.0795CuSO4·5H2O0.03ATUg·L-10.0021KI0.18g·L-10.048MnCl2·4H2O0.12mL·L-10.525Na2MoO4·2H2O0.06mL·L-10.575ZnSO4·7H2O0.12pH7.0CoCl2·6H2O0.15EDTA10　　,,.2,5,,COD800mgL.1～2.2　5mg·L-1Table2　Operationalmodelmg·L-11234580060040020000200400600800　　SBR.4L,7500mgL.1.3　COD5B-1A;PO3-4-P-;MLSS;SVI[17].,.BX-51,(、、、、Beun[5].OURStrathtox.PHABond[18].,24h,,2mL2mL(3%),22438:105℃,1mL,30min,,6890.Crocetti[19]FISH.4%PFA,4℃2～3h.10min,,50%、80%98%3min.0.9molLNaCl、20mmolLTris-HCl,1%SDS,pH7.2.,46℃2h.3.,48℃20min.,20～25(IPP7.0Software).3　FishTable3　16SrRNA-targetedoligonucleotideprobesusedintheexperimentFA%EUB338—FITCEubacteriaGCTGCCTCCCGTAGGAGTPAO46235Cy-3CCGTCATCTACWCAGGGTATTAACPAOmix1)PAO65135Cy-3AccumulibacterphosphatisCCCTCTGCCAAACTCCAGPAO84635Cy-3GTTAGCTACGGCACTAAAAGG1)PAOmixPAO462∶PAO651∶PAO846=1∶1∶1[20]2　PAO,,,SBR,,3.2.1　SBR,11,15min,,,,,SVI120mLg,.,,(DO6mgL),(2d1,,COD,1dCOD800mgL600mgL,).,,,,6000mgL.2.2　PAO1,(15min),,,.8,,,,,(0.5mm),,,,,SVI20mLg.23.2　Fig.2　Observationofgranularsludgebyeyes　　,4.,,,,,[21]PAO.,COD95%,97%.PAO.5,.2244　　　　　　293　(×200)Fig.3　Configurationofgranularsludge(×200)4　(×5000)Fig.4　Bacteriainsidethegranules(×5000)5　SBRFig.5　PollutionremovalandpolymerformationofSBRinatypicalcycle5,.,PAO,PHA.,.,PAOPHA.PAO.2.3　PAO,FISH.,PAO,,GAO,GAO,COD,PHA,,,GAOPHA,PHA.GAOPAOGAO,,,GAOPAO,.EBPRGAO.FISH,GAO,,4,GAO———AlphaproteobacteriaGAO,PAO,,AlphaproteobacteriaGAO,PAO.,AlphaproteobacteriaGAO,[22,23],PAO,2,,,,.6EBPR.6,,.120mgL200mgL.,COD,CODPAOPHB,,COD,.PAO,PAO.,(MLSS)0.05mgmg0.15mgmg,poly-P.152:12,FISH2PAO,7,,22458:,1PAO,,40%,2PAO70%～80%,PAO,.,PAO.6　Fig.6　PerformanceofphosphorusremovaloftheSBRsystem7　12FISHFig.7　FISHanalysisofpoint1andpoint2　　,12,、、.2,,,,.,.128.8　(×40)Fig.8　Imageanalysisphotographsofthegranulesbymicroscopy(×40)indifferentcultureperiod8,2.5,0.5mm2mm,,,,,,,,.,,4.4　Table4　Performanceindexesofthegranulesindifferentcultureperiod12mm0.52cm·s-10.55OURmg·(g·min)-10.818.6g·cm-31.00341.0075%9897%68.386.9　　4,1,2,2,,,,,,[24].,2,,.2,,,,,,.3　PAO,PAO2246　　　　　　29.(1)　PAOSBR28[25],,.3000～4000mgL,6000～8000mgL.,,SBR710,.(2)　PAO,,,,SS,,,,,,SS.(3)COD　PAO,,,COD,COD,CODPAO,,,PAO,,,,.COD,COD,,、.4　(1),,SBR,,PAO.(2),GAO,PAO.(3)PAO,,COD94%,100%.(4)PAO2mm,,,,,,.(5)PAO,PAO,,COD,.:[1]　,.[J].,1999,20(2):38-41.[2]　,,.[J].,2003,24(4):99-104.[3]　LettingaG,vanVelsenA,HobmaS,etal.UseoftheUASBRconceptforbiologicalWWT,especiallyforanaerobictreatment[J].BiotechnologyandBioengineering,1980,22:699-734.[4]　BeunJJ,vanLoosdrechtMCM,HeijnenJJ.Aerobicgranulationinasequencin