改性壳聚糖CAD与微生物絮凝剂MBF8复配絮凝研究

3311201311ActaScientiaeCircumstantiaeVol．33No．11Nov．2013No．SQ2009AA06XK1482412SupportedbytheNationalHigh-TechＲesearchDevelopmentProgramofChinaNo．SQ2009AA06XK14824121986—[e-mail]*[e-mail]1986—[e-mail]*[e-mail]iae33112999-3006CADMBF8112*1．5100062．5106402013-02-262013-04-012013-04-01AM、DMDAACCAD．FT-IＲ、XＲD、zetaξ、CADpH3．72．88×10616.70．1%pH10．52．6%、、．CAD3．67×105MBF812～460NTU、3．0mmol·L－1．MBF8CADMBF8CAD5∶3pH6．0～10．0．PAC3.0mg·L－1+MBF8+CAD1．0mg·L－1pH=8．0110NTU＞97%＜0．08mg·L－1、PAC+800、1200PAM1500PAM．0253-2468201311-2999-08X703．1AFlocculationpropertiesofthecompoundbioflocculantYUQi1HUYongyou12*LEIZhibin1．CollegeofEnvironmentandEnergySouthChinaUniversityofTechnologytheKeyLabofPollutionControlandEcosystemＲestorationinIndustryClustersMinistryofEducationGuangzhou5100062．StateKeyLaboratoryofPulpandPaperEngineeringSouthChinaUniversityofTechnologyGuangzhou510640Ｒeceived26February2013receivedinrevisedform1April2013accepted1April2013AbstractCationicmodifiedchitosanCADwasmadebygraftingacrylamideAManddimethyldiallylammoniumchlorideDMDAACtothechitosanmoleculesthroughfreeradicalpolymerizationinaqueoussolution．CharacterizedbyFT-IＲXＲDandGelChromatographyCADwasfaintyellowtransparentliquidwithpH3．7relativemolecularmass2．88×106cationicdegree16．7isoelectricpoint0．1%solutionaboutpH10．5andeffectivecomponents2．6%．ThemainfunctionalgroupsofCADwerehydroxylaminoquaternaryammoniumgroupandamidegroup．ThemicrobialflocculantMBF8producedbyfermentationofaspergillusfumigatuswaspolysaccharideelectronegativitypolymerwithrelativemolecularweightof3．67×105．TheflocculationcharacteristicsofthecomplexofMBF8andCADwereinvestigatedbyflocculationofpairingkaolinsuspensionturbidity12～460NTUionicstrength3mmol·L－1inbeaker．TheresultshowedthatMBF8shouldbepreferentiallyaddedwithoptimumdosesratio5∶3andpH6．0～10.0．Underturbidity110NTUandpH8thecombinationofPAC3mg·L－1+MBF8+CAD1．0mg·L－1showedhighefficiencyforflocculationofkaolinsuspensionthatcouldremoveupto97%ofturbidity．Theresidualaluminumwaslessthan0．08mg·L－1．TheflocscanbesettledquicklyduetotheircompactclusteringandtheflocculationperformancewasbetterthanthatofPAC+cationicPAMwithmolecularweightbetween8millionand12millionandofPAC+anionicPAMwithmolecularweightof15million．Keywordsmicrobialflocculantmodifiedchitosancombinedflocculationflocculationproperties1IntroductionJarvisetal．20052011．、33Arezoo2002．Salehizadehetal．2001Yangetal．2009．、Heetal．2004Lietal．2009．、．Kim2011CaCl2、FeCl3．Zhao2012CBF-．2006、PAC、PAM60%～75%98．5%．Bo2011UV25423．8%7%．、Yangetal．2009、．．Wang2009DMC、COD99．4%、81．3%90．7%．Defang2007PAC、COD、SSAl3+1．8%～23．7%、50%61．2%～85．5%．20123-—2-CTAPAC＜2．00NTUCOD92．98%32．0%．、．AM、DMDAACCAD．CADAspergillusfumigatusMBF8Huangetal．2005．PAC+MBF8+CAD．2Materialsandmethods2．1PloyaluminiumChloridePACAl2O323．3%2．33．5．0g·L－1Al2O3．2012MBF8MBF83．2g·L－11．0g·L－1．200g500mL0．1mol·L－1NaOHpH7．5±0．11L12h800mL50g·L－1．100～110NTU．0．1mol·L－1NaNO3NaHCO33．0mmol·L－1．0．1mol·L－1NaOH0．1mol·L－1HClpH．≥95%100～200mPa·s≤1%≤2%≤10%．AM≥98%．DMDAACDADAAC60%．2．22．2．1CAD1%35℃5min0．7mL0．1mmol·L－115min10min60%3hCAD2．0g·L－1．2．2．2000311CADMBF8GPCCAD410250cNaCl=0.1mol·L－10．6mL·min－1．Cl－CADDC2006．CAD12h1∶100．XＲDBrukerADVANCEX．CuKαλ=0．15406nm40kV100mA4°·min－10.022θ10～55°．2．2．31L150r·min－1PACAl2O3mg·L－130sMBF830sCAD30s50r·min－115min10min．2100P．S．2．2．42．0min0．5cm．3Ｒesultsanddiscussion3．1CAD3．1．1CAD2．6%pH3．7GPCCAD2．88×10616．70．1%pH10．5．3．1．21CAD3410cm－1O—HN—HⅠ1666cm－11320cm－1—CC—CH1155cm－1C—O1076cm－1C—N952cm－1．CAD、、、．1CADFig．1FT-IＲspectraofCAD2CADX．CADFormⅠ2θ10°FormⅡ2θ20°AM、DMDAACCTSCTSCADWangetal．2008．2CADXFig．2X-raydiffractionpatternofCTSandCAD3．23．2．1pH=8．03.0mmol·L－13．0mg·L－1PACMBF80．5mg·L－1、CAD0．5mg·L－112～460NTU3．MBF8CAD＞CADMBF8＞MBF8、CAD＞MBF8、CAD．MBF8、CADMBF8100333CADMBF8、CADCADCADPACMBF8CADMBF8PACCAD．MBF8CAD．3Fig．3Theresidualturbidityofdifferentdosingsequences3．2．2pH=8．03.0mmol·L－1PAC3．0mg·L－1+MBF8+CAD110NTU4．MBF8ξCADCADCADξ．5∶3ξCAD0．5、1．0、2．0mg·L－13．66NTU、3．37NTU、3．25NTUMBF8、CAD5∶3．3．33．3．1pH=8.03．0mmol·L－1PACPAC1．0mg·L－1MBF8、CAD、MBF8+CAD5∶3PAC110NTU4MBF8∶CADFig．4Effectofflocculantmixingratioonflocculatingefficiency5．PACPAC+MBF8+CAD＞PAC+MBF8＞PAC+CAD＞PAC．PAC≥3．0mg·L－1+MBF8+CAD1．0mg·L－12．50NTU．PAC1．0～6.0mg·L－1+MBF8+CAD1．0mg·L－1PAC59%～85%．PAC+MBF8+CADPAC25%～75%．PAC+MBF8+CADMBF8CAD—OH、—NH2—CONH2H+、OH－Lietal．2009MBF8CAD—PO4—COOH—PO3－4—COO－PAC2012．MBF8CAD200311CADMBF8MBF8CAD．5PACFig．5EffectofPACdoseonresidualturbidity6pHFig．6EffectofinitialpHonflocculatingefficiency3．3．2pH3．0mmol·L－13．0mg·L－1PAC3．0mg·L－1PAC2．0mg·L－1MBF8、CAD、MBF8+CAD5∶3pH110NTU6．2．33PACLin2008PACpH8．0～10．020122013PAC+MBF8+CADpH6～1010．00NTUMBF8、CAD—NH2、—COOH、CADPAC+MBF8+CAD/．3．3．3pH=8.03．0mmol·L－1PAC3．0mg·L－1+110NTU7．0．2～1．8mg·L－1MBF8、CAD、MBF8+CAD5∶3PAC+MBF8+CAD＜PAC+CAD＜PAC+MBF8MBF8+CAD1．8mg·L－162．5μg·L－1200μg·L－1．PAC+MBF8+CADMBF8—OHPAC—OHPACCAD—NH2、—OHMuzzarellietal．1994Bassietal．2000．7Fig．7EffectofMBF8an