高氨氮垃圾渗滤液SBR法短程深度生物脱氮孙洪伟

　60　7　　　　　　　　　　　　Vol.60　No.7　20097　　CIESC　Journal　　　July　2009SBR孙洪伟,王淑莹,王希明,时晓宁,彭永臻(,100124):;;;;;FISH:X703.1　　　　　　:A:0438-1157(2009)07-1806-06AdvancednitrogenremovalfromlandfillleachatewithhighlyconcentratedammonianitrogenvianitriteinSBRSUNHongwei,WANGShuying,WANGXiming,SHIXiaoning,PENGYongzhen(KeyLaboratoryofBeijingWaterQualityScienceandWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing100124,China)Abstract:Thetreatmentoflandfillleachateobtainedfrommunicipalsolidwaste(MSW)byusingalab-scalesequencingbatchreactor(SBR)wasstudied.Theaimofachievingandstabilizingpartialnitrificationanddenitrificationandthecharacteristicsofadvancednitrogenremovalwereinvestigated.Theexperimentalresultsshowedthatfor95daysrunning,stablepartialnitrificationwithnitriteaccumulationrateabove92.5%wasachievedandmaintainedsuccessfully.ThestablebiologicalnitrogenremovalviapartialnitrificationanddenitrificationwasrealizedandtheaverageremovalefficiencyofNH+4-NandTNwereabove97.2%and91.7%,respectively.TheDO,ORPandpHcouldbeusedastheprocesscontrolparametersforSBRreactor,becausethecharacteristicpointsontheprofilesindicatedexactlythenitrificationanddenitrificationendpoint.Thenitriteoxidizingbacteria(NOB)wasmoresensitivethanammoniaoxidizingbacteria(AOB)tothefreeammonia(FA)andfreenitriteacid(FNA),sotheactivityofNOBwasinhibitedbythesynergeticeffectofhighlyconcentratedFAandFNAintheSBRnitrification.Furthermore,thekeyfactorofachievingandstabilizingpartialnitrificationanddenitrificationforalongtimewasoptimizationofnitrifyingmicrobialpopulationbytheassistanceofprocesscontrol,whichwasprovedbyFISHanalysis.Keywords:landfillleachate;sequencingbatchreactor;partialnitrificationanddenitrification;freeammonia;freenitrousacid;FISH　　2009-01-08,2009-02-24。:。:(1976—),,,。:(8091001)。　　,,、[1]。　　Receiveddate:2009-01-08.Correspondingauthor:WANGShuying,[e-mail]:supportedbytheKeyProgramofNaturalScienceFoundationofBeijing(8091001).　,“”,、。,2[(Nitrosomonas)(Nitrobacter)]2:;。、、[2]。,,、。,,[3]:①25%,;②40%,;③63%;④50%;⑤30%～40%。,。C/N、、pH。,(SBR),,SBR,SBR。,SBR,,SBR、。1　1.1　SBR,210mm,500mm,,9L,4,100mm。,(,),。,。SBR,SBR:,、,,、。SBR,、、CODDO、ORPpH。1.2　,1,,(738～1287mg·L-1),(SBR150～200mg·L-1),()。1　Table1　CharacteristicoflandfillleachateParameterRangeAveragepH7.1—8.57.8COD/mg·L-17856—2250015178TC/mg·L-13450—40103730TN/mg·L-1838—13901114NH+4-N/mg·L-1738—12871012NO-3-N/mg·L-12.6—9.66.1NO-2-N/mg·L-11.0—2.61.81.3　NH+4-N、NO-3-N、NO-2-N、COD、[4]。TN、TON、TC、TOC、TICTN/TOC(MultiN/C3000,)。WTWDO、ORP、pH。(FA)、(FNA)[5]FA=1714[NH+4-N]×10pHexp6334273+T+10pHFNA=[NO-2-N]exp-2300273+T×10pH　FA,mg·L-1;[NH+4-N],mg·L-1;T,℃;FNA,mg·L-1;[NO-2-N],mg·L-1。2　2.1　4L,·1807·　7　　:SBR4L,1∶2。1.0mg·L-1,20d,24d,NO-2-N,NAR,NAR=ρ(NO-2-N)ρ(NO-2-N)+ρ(NO-3-N)×100%89.4%,SBR,1。1　SBRFig.1　NitriteaccumulationinSBRatdifferentphases　SBR2。Ⅰ———,,NO-3-N。,NO-3-N,NO-2-N,,1d29%24d89.4%,。,14d,54%,,。Ⅱ———,24～43d,,86.6%,95.2%,52d。,SBR95d,。2.2　SBRSBR,SBR,17.1℃,,NH+4-N、NO-3-N、NO-2-NCOD(2)。2,NH+4-N165.6mg·L-1,,NO-3-N5.1mg·L-1,NO-2-N135.5mg·L-1,96.4%。,。,TN188.3mg·L-1,,TN15.6mg·L-1,91.7%,。TN,TN33.1mg·L-1,TN139.6mg·L-1,、TN19.2%80.8%。2　SBRFig.2　PartialnitrogenremovalduringtypicalrecycleinSBRreactor　2.3　DO、ORPpHSBR　　SBR,DO、ORP、pHCODNH+4-N、NO-3-N、NO-2-N,DO、ORP、pHSBR,SBR。SBRDO、ORPpH3,,,DO、ORPpH4,DO“DO(DObreakpoint)”,pH“(ammoniavalley)”,ORP“(nitriteknee)”,pH“(nitriteapex)”。“”。[6-7]。,,H+,pH。·1808·　　　　　60　,CO2,,CO2pH。DO,,,,DO。,ORP。,,ORPDO,,。,ORP,,,。pH,。,pH,“”,,pH。3　SBRDO、pH、ORPFig.3　TypicalvariationsofDO,pHandORPduringSBRpartialnitrogenremovalprocess　2.4　FA、FNA(FA)(FNA)[5]。(AOB),(NOB)FA、FNA[8-10]。FApH、,。FNApH、,pH、,。4SBRFA、FNA。4,165.6mg·L-1,pH8.94,17～17.9℃,FA46.9mg·L-1,SBR,NH+4-NpH4　SBRFA、FNAFig.4　VariationsofFA,FNAduringSBRpartialnitrogenremovalprocess　(),FA,0～455min,FA46.9mg·L-10.15mg·L-1。FNA1.9×10-6mg·L-12.63×10-3mg·L-1。,,FANOB,FNA。,SBR,,FANOB,FAFNA,FA、FNANOB。,SBR,,pH(),FNA,,NOB,。DO、ORP、pHSBR,,,NOB,NOBAOB,SBR。2.5　,FISHAOBNOB[11]。16SrDNA16SrRNA,,DNA,,·1809·　7　　:SBR2　FISHTable2　16SrRNA-targetedoligonucleotideprobesusedProbeSequenceSpecificityFluorescentlabelEUB338GCTGCCTCCCGTSGGAGTEubacteriaFITCNSO1225CGCCATTGTATTACGTGTGAammonia-oxidizingβ-ProteobacteriaCyt3NIT23CCTGTGCTCCATGCTCCGNitrobacteriaCyt3,DNADNA、,。2。,48℃20min。,20～25(OLYMPUSBX52)(LeicaQWINSoftware)。85dFISH,5,AOB9.6%,NOB,,AOB。3　　(1)SBR,95d,,92.5%。,91.7%TN,TN20mg·L-1,。(2)DO、ORPpHSBR、,SBR,。,,。(3),FA、FNA,SBR,FA,FNA,SBR。(4)SBR,FAFNA,,,NOB,“”,AOB,,,FISH。References[1]　WangBaozhen(),WangLin().TreatmentandDisposalofLandfillLeachatefromMunicipalSolidsWaste().Beijing:ChemicalIndustryPress,2005[2]　Metcalf&Eddy,Inc.WastewaterEngineeringTreatmentandReuse.4thed.USA:McGraw-HillCompanies,2003[3]　TurkO,MavinciDS.Selectiveinhibition:anovelconceptforremovingnitrogenfromhighlynitrogenouswastes.Environ.Technol.Lett.,1987,8:419-426[4]　NationalEnvironmentalProtectionAgency().StandardMethodfortheExaminationofWaterandWastewater().3rded.Beijing:ChineseEnvironmentalSciencePress,1998·1810·　　　　　60　[5]　AnthonisenAC,LoehrRC,PrakasamTBS,etal.Inhibitionofnitrificationbyammoniaandnitrousacid.J.WaterPollut