高等微生物活性测定方法的实验研究

29120091ActaScientiaeCircumstantiaeVo.l29,No.1Jan.,2009:(No.50678017);(No.03CDP008);(No.BJE10016200611)SupportedbytheNationalNaturalScienceFoundationofChina(No.50678017),theRoyalDutchAcademyofSciences(No.03CDP008)andtheFundingProjectforAcademicHumanResourcesDevelopmentinInstitutionsofHigherLearningUndertheJurisdictionofBeijingMunicipality(No.BJE10016200611):(1960)),,(),E-mai:[e-mail];*()Biography:HAOXiaodi(1960)),male,Professer(Ph.D.),E-mai:[e-mail];*Correspondingauthor,,,.2009.[J].,29(1):95-101HaoXD,ZhuJY,CaoXQ,etal.2009.Experimentalevaluationofamethodtodeterminetheactivityofhighermicroorganisms[J].ActaScientiaeCircumstantiae,29(1):95-101郝晓地*,朱景义,曹秀芹,曹亚莉北京建筑工程学院可持续环境生物技术研发中心,北京100044:2008-01-31:2008-09-23:.,..SBRBCFS,.,SBR,14%.BCFS,BCFS,.:;;;;(OUR):0253-2468(2009)01-95-07:X172:AExperimentalevaluationofamethodtodeterminetheactivityofhighermicroorganismsHAOXiaodi*,ZHUJingy,iCAOXiuqin,CAOYaliTheR&DCentreforSustainableEnvironmentalBiotechnology,BeijingUniversityofCivilEngineeringandArchitecture,Beijing100044Received31January2008;accepted23September2008Abstract:Highermicroorganismsplaysignificantrolesinbiologicalwastewatertreatmentsystems.However,therearestillnoeffectivemethodstodeterminetheactivityofhighermicroorganisms.Inthisarticle,amethodinvolvingsaltinhibitionofhighermicroorganismsisintroduced,andtestedwithsludgesamplesfromalab-scaleSBRnitrifyingsystemandapilot-scaleBCFSsystem.Theexperimentalresultsrevealthatthismethodissuitabletodeterminetheactivity(14%)ofhighermicroorganismsfromtheSBRnitrifyingsystem.However,itisinappropriateforthosefromtheheterotrophicBCFSsystembecausetheheterotrophicbacteriaintheBCFSsystemcouldacceleratetheirinternalsubstrateconsumptionratetofightagainstthestressinducedbysaltaddition,whichresultedinincreasedtotaloxygenuptakerate(OUR)ofthesludgesamples.Keywords:biologicalwastewatertreatment;highermicroorganisms;salt;activity;oxygenuptakerate(OUR)1(Introduction),.,,,,,.,.,,(Weietal.,2003).,.,Ghyoot(Ghyootetal.,2000).,,29.,,.(,cycloheximidenystain),(Leeetal.,2003;Leeetal.,1994).,,.(Liangetal.,2006).,,.Moussa,NaCl(Moussaetal.,2005),(OUR).,,.,.,,.NaCl.2(Materialsandmethods)2.1SBR硝化系统SBR(,2008).SBR5L,4L,HRT12h,SRT10d,6h,4.6(1).1SBRTable1CyclingperiodoftheSBRnitrifyingsystem0y1min1y6min6y286min286y287min287y350min350y360minSBR,(30.0?0.5)e.pHDO;pH7.30?0.05,pH1mol#L-1NaOHpH7.3;DODO.pHDO.SBR.;,NH4C.lNH+4-N200mg#L-1.2.2BCFS系统BCFS.BCFS-5,40L6L40L120L120L.0.42m3#d-1,HRT18h,SRT15d.,COD400mg#L-1,NH+4-N40mg#L-1,TP8mg#L-1.MLSS4000mg#L-1.2.3加盐(NaCl)抑制高等微生物1~2h,,.,,,.NaClCl-,5g#L-110g#L-1.,,.,.,,1h.,,,30min,1~2,.,..,,pH,pHOUR.,Na2CO3NH4Cl(Moussaetal.,2003),Na2CO30.01mol#L-1.,pH,BCFS.961:,.5min30min1h2h6h.2.4高等微生物观测和计数.25LL(Zeiss,Axioskop40),.5,.2.5高等微生物活性测定OUR.OUR,DO,5~6,,DO6mg#L-1.OUR(YSI,5100),,.,,30e20e.,5min.OUR;OUR,3mLNH4Cl,NH+4-N100mg#L-1(),BCFS6mLNaAc,COD400mg#L-1(BCFSoRCOD).3(Results)3.1硝化系统SBR30d.DO(1),DO;(MLSS),(870?10)mg#L-1;(SS),(17?3)mg#L-1.1SBRDOFig.1DOconcentrationsoveronetypicalcycleoftheSBRnitrifyingsystem2SBRNH+4NO-2NO-3.,NH+4NO-2.,NH+454.8mg#L-1#h-1;NO-234.7mg#L-1#h-1.2SBRNH+4NO-2NO-3Fig.2NH+4-N,NO-2-NandNO-3-NconcentrationsinonetypicalcycleoftheSBRnitrifyingsystem3.2高等微生物观测计数和盐的抑制作用3a,SBR,,.BCFS.,SBRBCFS770cell#mL-1(8.8@105cell#g-1)3.6@103cell#mL-1(9@105cell#g-1).,gMLSS.,.3bSBR5min.,,.,.,.(Moussaetal.,2005),.,.,,,2h.,.Salem(Salemetal.,2006)Salvando(Salvandoetal.,2001).Salem9729;Salvando,25g#L-1,24h.3SBRFig.3EffectofsaltonhighermicroorganismsintheSBRnitrifyingsystem4SBRFig.4OURofsludgesamplesfromtheSBRnitrifyingsystem(treatedwithandwithoutsalt)3.3高等微生物活性测定3.3.1SBR硝化系统4SBR.5g#L-1,1h.42,SBR,,14%.,.,,,.,.,2,,..2SBRTable2OURofsludgesamplesfromtheSBRnitrifyingsystemSBR()OUR3.96mg#L-1#h-1(100%)59.7mg#L-1#h-1(100%)OUR3.42mg#L-1#h-1(86%)58.5mg#L-1#h-1(98%)3.3.2BCFS系统5BCFS.,DO.,,,DO;,,,.5g#L-1,1h.3,,,12%..,,,.,DO,.,,.,,.5BCFSFig.5OURofsludgesamplesfromtheBCFSsystem(treatedwithandwithoutsalt)3BCFSTable3OURofsludgesamplesfromtheBCFSsystemBCFS()OUR12.24mg#L-1#h-1(100%)83.52mg#L-1#h-1(100%)OUR13.68mg#L-1#h-1(112%)86.24mg#L-1#h-1(103%)981:67.,,.,.,.8.,1h,.,2h,.3h,,.4(Discussion),.SBR14%,,BCFS,.,.,.4.1问题1,SBRBCFS?9SBRBCFSFig.9MicroorganismsintheSBRnitrifyingsystemandtheBCFSsystem.(9),SBR,;BCFS,.,DO:,.,9929,,.,.,,.,DO.,,(),.,(),SleatorHill(Sleatoretal.,2001).,,.,.,.,.,.SBR,,.,SBR,.,,.BCFS,,Feas-tFamine,,,BCFS.,BCFS.,SBR,.,.BCFS,,,,.,.,,,.SBRBCFS.4.2问题2,,BCFS?,,.,.,..,.9,2h3h,..5(Conclusions)1)(NaCl),,..2),,2h.3),NH+4..SBR14%.4),,.,DO,1001:.(References):GhyootW,VerstraeteW.2000.Reducedsludgeproductioninatwo-stagemembrane-assistedbioreactor[J].WaterRes,34(1):205)215,,,.2008.AOBNOB[J].,28(12),2499)2502HaoXD,ZhuJY,CaoXQ,etal.2008.Experimentaldeterminationofthedecayrateofnitrifyingbacteria:AOBandNOB[J].ActaScientiaeCircumstantiae,28(12):2499)2502(inChinese)LeeY,OleszkiewiczJA.2003.EffectsofpredationandORPconditionsontheperformanceofnitrifiersinactivatedsludgesystems[J].WaterRes,37(17):4202)4210LeeNM,WelanderT.1994.Influenceofpredatorsonnitrificationinaerobicbiofilmprocesses[J]