高丁醇比丙酮丁醇梭菌的选育与应用张益棻

19962641.(200302),,、,,。、、、。,70%,,394o0C,,。,,71.9%,。:,,(Clo:farerobot21-`:),80。,、『。,60,,。70,,,,,。(199019)(GenetiesofIndustrialMieroor-gaulsms),、,【.,。,,,,。`·”。、,、,。,,209/L,71.9%。1.1.11.1.20.5%,1%,0.5%,0.5%,0.5%,2.5%,PH6.5!.1.34%,0.6%,`·,219962640.3%,0.2%,MgSO`·7HZO0.02%,KZHPO。0.05%,FeSO`·7H:00.001%,PH6.51.1.47%8%1.2(1)64。(2),cIm。。,50,,,3o7C。(3),,,5%,37oC。(4),,5%,3o7C,。1.3(EMS)(NTG)。1.3.1EMS107/ml,sml,mlo.2MEMS,,,37℃Zh。,50g/ml。37℃%h。1.3.2NTGNTG,37oC%h。1.`1.4.1,(102,)lm,20。℃,,CDMCIB。1.4.2()UV120〔),O..D200nm。1.4.3PHpH(pHS2)。1..410ml30ml,2,0.INNaOH,。1.4.5,280100PorapakQ,200oC,,CDMCIB。1..6:(),,,。UV120(),0.D.550nm。22.1、641200,,4。49.699/L,66%。EMSEB20,13.099/L,67.2%。E2B0EMSNTG,EA2018、EA2019、32198811、32198814-991626434.(9.69/,66%)EMSEMS+EB20—,—,—,20(13·099/L,67.2%)EMSEAZ018EA20192024473NR(19.1639,`L(20.5592`L69。7%)68.17)15265193425,2018、2019。。,,,。,,,,7tJ。,,RogersDaI0saari”,,,,。,,。,,。2.2,、。,。1EA2018EA2019sh1g/LLLL%ùōno001.,Róù`4,......……n.nóOUOUnU0QUQOOOU11ù`n.0。530。570。640。670。750。730。640。650。710。640。660。6611。6911。8711。8612。7714。2414。5211。6012。2413。9713。1212.2113。2516。6916。9016。9918。2920。3720。7016。661。6120。0618。9017。5118。89ūOJJJ00..…,22ttJ,OdJ,d`.1bOUddùóóJ,ald123456213465EA2018EA201941996264,7%,189/L,70%,。2.3,20h,,,,、,50h。1,,,,-—.~0261,/`。,L)(,21)0.2/(g).154O1(h)1EA2018。2.4,,。,20%。EA2018EA20192。,。2.537℃,,,。2g/Lg/Lg/LL%3Lg/LLL%g/LLO`.J,…O`.OtOEA20184。601。0112。9514。520。9812。9214。291。0312。9614。291。0013。1118。588。43EA20198。8684069。770。270。871。337oC39oC40oC4。844454。45。67。86。3012。6013。0511。9118。1118。3617。68:EAZO19,7%48h2EA2018EA2019,3EA20191996264639oC,40oC2019EA2018。100120。2.684。4(1994819954).’0..0.n`O.Jnó,,OOUJn`UóXù1OOU....……JóóùóJJJó``JnJ,U54RRù2458-ǎ4,J,,dl`dl01QtUIJO`OnUOnóOó,`d009ù.......……,`.00OUn”OkJ00RlOnUoUJn`,ù,00,J0.2..,O,.blùrJO,,,`JOU、,J、.、.`、.`、、.、.、``.、.、.、.`JI,l`11`jl12`1212.、.、.J`、.、、、,.、J、.、r、26。2423。5222。2422。332。90233。236。69385。820568。21969。6076。18116。951117。101115。13314。8957。6128。327。20412。1610。3411。532OóóOUgOUU.……QùOU`.2ZnùObn,,édùūd.……n`,1ēùùUOn``,n`O`:n,10`:OUQU1.1,,Q.……J,JJ`“JOOUOUQUOO95。3。954。26。52221。52118。918。2105。48088。60975。08。5874。88。291245。51420。95843。099571。45】80。61511。77。78。36。87。56。47。16。17。07。76................adlbJū9ó,ù9ùUēUēUnóO-:``,ó,(1)(2)EA2018:1)10%。2)0.5%,75kg。3)20%50%,,,300。EA2018,,,6000。958,6872%。2·7,,,20%,30oC。:,S%(6%),3C48h,。[1〕Hastings,J.J.H.AdvancesApPliedMrobiolog。1971,vol.14,I45.[2〕Walton,M.T.andMartin,J.L.MierobialTeehnolog.Zdned.,1979,vol·1,187we209。[8〕MeNeil,B.adnKristiansen,B.AdvancesinAPpliedMrobiolog,1986,vol.31,6291。[〕Rers,P。AdvancesnAPPIMieorbiolv,1986,vol。31,160.〔5〕Jones.D.T.andWoods,_D.R,ro-IOgfcal.,,1986,50,484524。[6〕eHslot,H。etal.Pcoreedings,6thInternationalSvmposiumoneGnetiesofIndustrialMiroosganms,vol。11,715729,731745,747757,759770,SocieteFraneaosedeMieorbiologie.[7]Rogers,P.andPalosaari,N.,1.,,1.,1987.53.2761276,61996264BreedingofHighRatioButano!StrainsofClsotrid1’cumaeetobull’cumandAPPliaetiontoIndustriaPlroduetionhZangYifen,ChenJun,YangYunliu,JiaoRuishen(DepartmentofMierobioly.ShanghaiInstituteofPlantPhysiology,Shanghai200032)AbstraetSolventProdueingstrainsofClostmacerobutylicumwereisolatedbyasPeeifieanaerobiesystemandPurifiedbyrePeatedsingleeolonyPlatingteehni-ques.ThePrimaryisolateswerethenmutatedwithNTGandEMS,andseleetedtoobtainhighratiobutanolstrains.Theeulturestablity,fermentatloneourses,effeetoftemperature,andmixedsubstrateswerestuded.Thebutanolyejdamoz:ntedto70%ofthetotalsolventwhiehreaehed189/Lfna7%eornmash.Fermentationanalyslsdemonstratedthatalleultures(rePresentedby2018)wasatyP:eallCostrmacetob“tylfcumfermentation.Besideseorn,eheePerrawmaterialssuehassweetpotatoehipsandsorghumcouldreplaeepartoftheeornwithanequalhighyieldofbutanol.Industrialapplieationoftheeulturein100tonfermentersforaPeriodof8monthsdemonstratedthestabilityoftheeultureandthebutanolamountedto67.775.0%withanaverageof71.9%.Keywords:Closrridiuacerobutlicu,Hghbutanolyieldingstrain,Solventfermentation、、、、、J、、、、、、、、J、ZV、、、、J、、、、、、J、Z、v、J“,22’,,LZ、,、、、19%11。,,,,。。、、、,,、。。()