高浓度甲醇废水厌氧处理中颗粒污泥和产甲烷细菌的耐酸性

:025322468(2004)0420633204:X703.5:A1,2(11,200092;21,150090):UASB,,.,UASBpH,4.95.85.5612,,pH.,,pH5.0,,.,,pH4.5..:;;;;UASB;Acidresistanceofmethanogenicbacteriaintwo-stageanaerobicprocesstreatinghighconcentrationmethanolwastewaterZHOUXuefei1,RENNanqi2(11StateKeyLaboratoryofPollutionControlandResourceReuse,TongjiUniversity,Shanghai200092;21SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090)Abstract:Two2stageanaerobicsystemwasemployedtotreathighconcentrationofmethanolwastewater.TheresultsshowedthepHchangedfrom4.9to5.8inthefirstUASBand5.5to6.2inthesecondreactor.Apparently,thesewerenotheadvisablepHlevelsthatcommonmethanogenicbacteriacouldaccept.Themethanogenicbacteriaofthesystem,viz.Methanosarcinabarkeri,hadsomeacidresistanceandcouldstilldegrademethanolatpH5.0.Iftrainedfurther,theiracidresistancewouldbeimproved.GranularsludgeofthesystemcouldprotectthemethanogenicbacteriawithinitsbodyagainsttheimpactofacidenvironmentandmakethemdegrademethanolatpH4.5.Theperformanceofgranularsludgewasattributedtoitsstructure,bacteriaspeciesandthedistributingofbacteriuminsidethegranule.Keywords:methanolwastewater;methanogenicbacteria;acidresistance;granularsludge;UASB;batchcultureexperiment:2003208206;:2003211214:(99099):(1971),,(),,,[1,2].::4CH3OH3CH4+CO2+2H2OGo=-312kJmol-1(1):4CH3OH+2CO23CH3COOH+2H2OGo=-2.9kJmol-1(2)CH3COOH+H2OCH4+CO2+H2OGo=-31.0kJmol-1(3),,,.Lettinga[3],,24420047ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.4July,2004COD17.5kg(m3d)-1,;,pH5.56.0,,[4].,,.,UASB,pH,.11.1UASB,1UASB(U1)10L,4.1L;2UASB(U2)18.4L,12.5L,(35+1),1.1.2.U13.4.U25.6.7.1Fig.1schematicdiagramoftwo2stageUASBprocessesUASBUASB,VSSSS0.42.NP,CODNP=(200300)51,pH7.0.,COD800020000mgL-1,1218h.UASB20gL-150gL-1.1.22UASB,,2.10150mLAB,2Fig.2Setupofbatchcultureexperiment,150mL,15mm,A;B34mm,300rmin-115min,,0.5mm,,.,6000mgL-1.pH410,415,510,515,6105,pH.(351),10min,.1.3(SX240)(H27000).4362422.13.,COD2618kg(m3d)-1,,90%;,COD.UASBpH4,pH710,UASBpH,90%.,pH,.3Fig.3Theperformanceofanaerobicsystem4pHFig.4ThediagramofsystemspHatvariousphase2125pH(A;B,,SS49122gL-1,VSSSS0159)Fig.5EffectofpHonmethanogenicbacteria5364:,pH615715,pH6.,U1U2pH5515,.,().5a,pH515610,;pH,.pH(5b5c),,,.pH415,,3h;pH410,115h,.6UASB(7000)Fig.6MorphologyforbacteriaongranulesludgeintheUASB(7000)5d,pH,,pH415,COD26%16%,..,,,6.,,,,.3UASB,COD2618kg(m3d)-1,,UASBpH,419518515612,pH.(),pH515610,pH510.,pH415,pH515,,.:[1].[M].:,1998:453454,503507[2]WeijmaJ,StamsAJM.Mehtanolconversioninhigh2rateanaerobicreactors[J].WaterScienceTechnology,2001,44(8):714[3]LettingaG,VanDerGeestATH,HobmaS,etal.AnaerobicTreatmentofMethanolicWastes[J].WaterResearch,1979,13:725737[4],,.[M].:,1993:23123863624