高效亚硝酸型反硝化菌生长特性及脱氮研究

49220093JournalofDalianUniversityofTechnologyVol.49,No.2Mar.2009:100028608(2009)0220180207,3,(,116024):,,.,,.,,;,(TOC)(N)=4,30,pH=9.,0.25h-1,610;,13%;,..:;;;:X172:A:2007202219;:2009201206.:(19802),,;3(19562),,,.0,,..[12],40%25%30%40%,,,[34].,,.,NO-2.:(1);(2)O2NO-2NO-3;(3)[5].,.,60,3:PseudomonasAlcaligenesBacillus[6],.,(nirSnirK)[78],.,,,,.11.1,,,,15.,100mL,10%,,307d,1mL,,1,.1.21.2.1NaNO21g/L,2.2g/L,K2HPO40.5g/L,MgSO47H2O0.4g/L,NaCl1.0g/L,pH7.27.4.1.2.2NaNO20.5g/L,2.45g/L,K2HPO40.5g/L,MgSO47H2O0.4g/L,NaCl10g/L,pH7.27.4.1.3,,100mL100mL,,,30.,[9].1.4,,100mL100mL,0.1g,,30.NO-22N,[9].1.5[10]N(12)2,NO-22N,pH.22.1,,,pH[11].,,,..2.1.1,,,[12].16(rg),1.100mg/LNO-22N,(TOC)(N)=6,,.116Tab.1Growthrateofthedenitrifierusing16differentcarbonsourcesrg/(mgL-1h-1)rg/(mgL-1h-1)1.1340.1410.7300.2190.9530.6460.5810.1370.3290.0030.2800.0010.3300.00250.4250.00081,,.,,,1.134mgL-1h-1;.,,1/4;.,.,,.Blaszczyk[13],,,.Martienssen[5],,,[14].2.1.2,,(TOC)/(N),.,N2O.,,:(),,;(),.():cm=0.5c(NO-22N)+1.75c(NO-22N),,1812:2.251,40%.(TOC)/(N)(1),(TOC)/(N)1,,,,.2(TOC)/(N)=4(q),(TOC)/(N)1,,.2.1.33,,2040,30.30,,,,302034;20,,,;30,,,,40,(rg)(rd)0.65mgL-1h-117.2mgL-1h-1;,,[15].3Fig.3Effectoftemperatureongrowthanddenitrificationrateofthedenitrifier2.1.4pH4,,.,pH810,9.0,[1516]pH7.08.5.,,.4pHFig.4EffectofpHongrowthofthedenitrifier2,pH.pH69,;pH=710,pH=91/2;pH7pH10,28149,pH=6.pHNO-2HNO2,HNO2,ATP,ATP,HNO2NON2O[17].pH10,,.2pHTab.2EffectofpHonspecificdenitrificationrateofthedenitrifierpHq/h-1pHq/h-16.009.00.257.00.1110.00.098.00.1911.00.048.50.232.1.5,,.,5,300mg/L.,,,60mg/L.,,,,,.5(a),,pH8.010.0.,;,,[18].5Fig.5Growthcurvesandnitritereductioncurvesofthedenitrifier2.2.1%3%,,(6),1%(7).4%9%,,3%10h,,.9%,..6Fig.6EffectofNaClconcentrationongrowthofthedenitrifier3812:7Fig.7EffectofNaClconcentrationonspecificdenitrificationrateofthedenitrifier2.3,,,,,,[19].Andrews[20]:q=qmaxSS+Ks+S2/Ki(1),,8,((2)).q270.86mg/L.q=0.9374SS+408.79+S2/179.47(2)8Fig.8Simulationcurveofnitritereductionkinetics2.4.1.2L,,0.8kg/L..:8h,100mL/h,(282),00.02mg/L.,.(9),,,.9Fig.9SEMphotoofceramicgranuleafterstartupphase10,,5d.200mg/L,10Fig.10Performanceofdenitrificationofpacked2bedbiofilmreactor4814957.6kgm-3d-119.2kgm-3d-1,(TOC)/(N)1264,0.8mg/L,.(TOC)/(N),COD.(TOC)/(N)=3,,,.(TOC)/(N)=4,200mg/L400mg/L,COD,.3(1),:,(TOC)/(N)4,30,pH9.0..(2),,610,,7%,0.13h-1,.(3),:qmax0.9374h-1,Ks408.78mg/L,Ki179.47mg/L,q270.86mg/L,3000mg/L.(4),14.457.6kgm-3d-1,2.49.6kgm-3d-1,,.NO-22NN2,,,,..:[1]POTTERTG,TSENGCC,KOOPMANB.Nitrogenremovalinpartialnitrification/completedenitrificationprocess[J].WaterEnvironmentResearch,1998,70(3):3342342[2]LADANH,GEORGEN,AMARJEETSB,etal.LongtermperformanceofMBRforbiologicalnitrogenremovalfromsyntheticmunicipalwastewater[J].Chemosphere,2007,66(5):8492857[3]RUIZG,JEISOND,RUBILARO,etal.Nitrification2denitrificationvianitriteaccumulationfornitrogenremovalfromwastewaters[J].BioresourceTechnology,2006,97(2):3302335[4]TURKO,MAVINICDS.Maintainingnitritebuild2upinasystemacclimatedtofreeammonia[J].WaterResearch,1989,23(11):138321388[5]MARTIENSSENM,SCHOPSR.Populationdynamicsofdenitrifyingbacteriainamodelbiocommunity[J].WaterResearch,1999,33(3):6392646[6]CHENEBYD,PHILOPPOTL,HARTMANNA,etal.16SrDNAanalysisforcharacterizationofdenitrifyingbacteriaisolatedfromthreeagriculturalsoils[J].FEMSMicrobiologyEcology,2000,34(2):1212128[7]BRAKERG,ZHOUJ,WUL,etal.Nitritereductasegenes(nirKandnirS)asfunctionalmarkerstoinvestigatediversityofdenitrifyingbacteriainPacificNorthwestmarinesedimentcommunities[J].AppliedEnvironmentalMicrobiology,2000,66(5):209622104[8]CASTRO2GONZALEZM,BRAKERG,FARIASL,etal.CommunitiesofnirStypedenitrifiersinthewatercolumnoftheoxygenminimumzoneintheeasternSouthPacific[J].EnvironmentalMicrobiology,2005,7(9):129821306[9].[J].,1994,15(2):29244[10].:3[M].:,1989[11]BAUMANNB,SNOZZIM,ZEHNDERAJB,etal.InhibitionofdenitrificationactivitybutnotofmRNAinductioninParacoccusdenitrificansbynitriteatasuboptimalpH[J].AntonievanLeeuwenhock,1997,72(3):1832189[12]BLASZCZYKM.EffectofmediumcompositiononthedenitrificationofnitratebyParacoccus5812:denitrificans[J].AppliedEnvironmentalMicrobiology,1993,59(11):395123953[13]BLASZCZYKM,MYUCIELSKIR,JAWOROWSKA2DEPTUCHH,etal.Effectsofvarioussourcesoforganiccarbonandhighnitriteandnitrateconcentrationsontheselectionofdenitrifyingbacteria:stationarycultures[J].ActaMicrobiologicaPolonica,1980,29(4):3972406[14]WILDERERPA,JONESWL,DAUU.Competitionindenitrificationsystemsaffectingreductionrateandaccumulationofnitrite[J].WaterResearch,1987,21(2):2392245[15],,,.[J].(),2002,25(3):81284[16].[M].:,1988[17]ALEFOUNDERPR,GREENFILAJ,MCCARTHYJEG.Selectionandorganisationofdenitrifyingelectron2transferpathwaysinParacoccusdenitrificans[J].BiochimicaetBiophysicaActa,1983,724(1):20239[18]PAYNEWJ.Denitrification[M].NewYork:Wiley,1981[19]JULIANC,IRENEJ,LORENAC.Kineticmodelsfornitrificationinhibitionbyammoniumandnitriteinasuspendedandanimmobilizedbiomasssystems[J].ProcessBiochemistry,2004,39(9):115921165[20]ANDREWSJF.TheMonodequation:arevisitandageneralizationtosubstrateinhibitionsituation[J].Biotechn