高效厌氧氨氧化颗粒污泥脱氮特征及EPS分层特性王衫允

EPS1232323231231．1500902．1001243．100124、EPS。NH+4－NNO－2－N0．140．15g/gVSS·d80．1%97．0%TN65．8%N2O0．92mg/LTN2．52%pH。EPSEPS226．9mg/gVSSEPS58．9%EPSTB－EPSEPS77．1%。TB－EPS165．546．1AU/mgC·L。。[e-mail]anceandEPSCharacteristicsofAnammoxGranularSludgewithHighActivityWANGShan-yun1JIAFang-xu23JINPeng-fei23GAOMeng-jia23MABin23PENGYong-zhen1231．StateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentHarbinInstituteofTechnologyHarbin150090China2．KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentＲecoveryEngineeringBeijingUniversityofTechnologyBeijing100124China3．EngineeringＲesearchCenterofBeijingBeijingUniversityofTechnologyBeijing100124ChinaAbstractThenitrogenremovalperformanceEPScharacteristicsandthree-dimensionalfluores-cencespectrumofAnammoxgranularsludgeforthetreatmentofwastewaterwithlowammoniaconcentra-tionwereinvestigated．TheresultsshowedthatAnammoxgranularsludgeexhibitedhighactivityandtheaveragedegradationratesofNH+4－NandNO－2－Nwere0．14g/gVSS·dand0．15g/gVSS·dwithremovalratesof80．1%and97．0%respectively．TheremovalrateofTNwas65．8%．Inthis·53·321120166CHINAWATEＲ＆WASTEWATEＲVol．32No．11Jun．2016processN2Owasproducedwithshort-timeaccumulationandthepeakconcentrationwas0．92mg/Laccountingfor2．52%ofTNconversionrate．InadditionpHvaluecouldindicatetheendofAnammoxreaction．TheanalysisofdifferentlayersofEPScomponentsshowedthatprotein226．9mg/gVSSwasthemaincomponentinallEPSlayersaccountingfor58．9%ofthetotalEPS．TB-EPSaccountedfor77．1%ofthetotalEPS．Three-dimensionalfluorescencespectrumanalysisshowedthattheproteininEPSwasmainlytyrosineandtryptophanwiththefluorescenceintensityof165．5AU/mgC·Land46．1AU/mgC·Lrespectively．TheseresultscanprovidetheoreticalbasisforthefuturestudyonAnammoxgranularsludge．KeywordsAnammoxgranularsludgePCＲ-clonelayeredextracellularpolymericsub-stancesEPSthree-dimensionalfluorescencespectrumAnammoxN2O12。13。34。56。EPS。EPSSlime、LB－EPSTB－EPS7。ChenTang56EPS。EPSEPS。UASB、、N2OpHEPS。11.1UASB。3．8L19∶1。30±1℃pH。NH+4－NNO－2－N31．6±3．731．2±2．9mg/L。1.21000mLSBＲ1。2～31000mL95%Ar+5%CO215minNH+4－NNO－2－N30mg/L8。30℃30minNH+4－N、NO－2－NNO－3－N5mL。N2OpH。1SBＲFig．1SchematicdiagramofSBＲsystemforcharacteristicsofN-removal·63·3211．watergasheat．com1.3NH+4－N、NO－2－NNO－3－N。VSS、COD9。TIC、TOC、TC0．45μmTOC。N2OUnisenseN2O。pHWTW。1.41.4.17。1.4.2EPSFolin－DNADNA10。1.4.3EPSFP－650011。200～400nmEx290～550nmEm51nm。TOC10mg/L。22.12。2Fig．2Characteristicsofhigh-NremovalbyAnammoxgranulessludgeMLVSS1356mg/LNH+4－NNO－2－N0．140．15g/gVSS·d80．1%97．0%NO－3－N0．07g/gVSS·dTN26．9%TN0．24g/gVSS·d65．8%。12。N2O。N2O150min0．92mg/L0．006g/gVSS·dTN2．52%N2O90min0．010g/gVSS·d。Ma3UASBN2O0．006%。－N2O5．1%～6．6%13。N2O14N2ON2。pH。。2.2EPS、DNAEPSTB－EPS296．8mg/gVSSEPS77．1%LB－EPSSlime66．122．1mg/gVSSEPS17．2%5．7%3。3EPSDNA、Fig．3ConcentrationoforganicsubstancesalongWASflocslayers3DEEMspectraofEPSfromdifferentlayersEPSSlime、LB－EPS、TB－EPS90．2%、90．6%49．5%。TB－EPS46%LB－EPS4．9%Slime。TB－EPSLB－EPS·73·．watergasheat．comEPS3211/0．930．05。DNAEPS4%。、DNA226．9、140．018．2mg/gVSSEPS58．9%、36．4%4．7%。EPSEPS56。EPSCa2+、Mg2+、K+15。4．7%2%～15%16DNAEPS。EPSPelletEPS38．5%。TB－EPS29．7%。TB－EPSPellet10。Li7SlimeLB－EPS。2.3EPSEPS4。4EPSFig．43DEEMspectraofEPSfromdifferentlayersEPS。EPSPeakⅠEx/Em=200～250/300～350nmPeakⅡEx/Em=260～290/300～350nmEPS。SlimePeakⅢEx/Em=200～250/380～450nmPeakⅣEx/Em=240～300/420～470nmEPS。SlimeLB－EPSTB－EPS、EPS。SlimeEPS。EPS。EPS。TB－EPS165．5AU/mgC·L46．1AU/mgC·LSlimeLB－EPS10AU/mgC·L。EPSEPSEPSTB－EPS。15。、。3①·83·3211．watergasheat．comNH+4－NNO－2－N0．140．15g/gVSS·d80．1%97．0%TN65．8%N2O0．92mg/LTN2．52%pH。②EPSEPS226．9mg/gVSSEPS58．9%EPSTB－EPSEPS77．1%。③EPSTB－EPS165．546．1AU/mgC·L。1KartalBvanNiftrikLKeltjensJTetal．Anammox—growthphysiologycellbiologyandmetabolismJ．AdvMicrobPhysiol201260211－262．2KuenenJG．Anammoxbacteriafromdiscoverytoappli-cationJ．NatＲevMicrobiol20086320－326．3MaBWangSCaoSetal．BiologicalnitrogenremovalfromsewageviaanammoxＲecentadvancesJ．Biore-sourTechnol2016200981－990．4LacknerSGilbertEMVlaeminckSEetal．Full-scalepartialnitritation/anammoxexperiences—AnapplicationsurveyJ．WaterＲes201455292－303．5ChenTTZhengPShenLD．Growthandmetabolismcharacteristicsofanaerobicammonium-oxidizingbacteriaaggregatesJ．ApplMicrobiolBiotechnol201397125575－5583．6TangCJZhengPWangCHetal．Performanceofhigh-loadedANAMMOXUASBreactorscontaininggran-ularsludgeJ．WaterＲes2011451135－144．7LiXLPengYZＲenNQetal．EffectoftemperatureonshortchainfattyacidsSCFAsaccumulationandmicrobiologicaltransformationinsludgealkalinefermen-tationwithCaOH2adjustmentJ．WaterＲes20146134－45．8VandeGraafAAdeBruijnPＲobtersonLAetal．Autotrophicgrowthofanaerobicammonium-oxidizingmi-cro-organismsinafluidizedbedreactorJ．Microbiolo-gy199614282187－2196．9．4M．2002．10．EPSJ．201233103522－3528．11KorakJADotsonADSummersＲSetal．Criticala-nalysisofcommonlyusedfluorescencemetricstocharac-terizedissolvedorganicmatterJ．WaterＲes2014491327－338．12．J．201434123078－3085．13DeslooverJDeClippeleirHBoeckxPetal．Floc-basedsequentialpartialnitritationandanammoxatfullscalewithcontrastingN2OemissionsJ．WaterＲes20114592811－2821．14．N2OJ．201466114653－466