高盐废水盐度对中度嗜盐菌生长动力学的影响宋柳琼

710Vol．7No．10201310ChineseJournalofEnvironmentalEngineeringOct．20131212*21．7300302．100085。Halomonassp．STSY-3OD600Monod。Halomonassp．STSY-37%NaClOD6002．560%34%OD6000．470．06Halomonassp．STSY-3μmax2．257h－1KS0．0082g/L。STSY-3STSY-332%～9%、9%～20%0．68%～1．76%≤2%≥20%。0．68%～1．76%。MonodX703A1673-9108201310-3755-05EffectofsalinityonmoderatehalophilicbacteriagrowthkineticsinhighsalinitywastewaterSongLiuqiong12ChangQing1TianBinghui2XinLihua21．SchoolofEnvironmentandMunicipalEngineeringLanzhouJiaotongUniversityLanzhou730030China2．ＲesearchCenterofEco-EnvironmentSciencesChineseAcademyofSciencesBeijing100085ChinaAbstractSalinitychangesseriouslyaffectthegrowthofmicroorganisminhighsalinitywastewater．ThestudytookamoderatehalophilicbacteriaHalomonassp．STSY-3asanexampletesteditsOD600intheendoflogarithmicphaseunderdifferentsalinitiesandtookglucoseastherestrictivesubstanceutilizedtheMonode-quationtofitthegrowthkinetics．TheresultsshowthattheoptimalgrowthsalinityofHalomonassp．STSY-3is7%inNaClmeterunderwhichitsOD600is2．56theOD600under0%salinityand34%salinityisonly0．47and0．06respectively．ThegrowthkineticsmodelofHalomonassp．STSY-3andtheexperimentaldatacanbewellfitted．Undertheoptimalsalinityitskineticsmodelparameterμmaxis2．257h－1KSis0．0082g/L．Com-paringthekineticsparametersunderdifferentsalinitiestheconclusionisthatthegrowthofmoderatehalophilicbacteriumSTSY-3hasthreeobviousregionsoptimumsalinityregionsalinity2%～9%halophilictransitionregionsalinity9%～20%and0．68%～1．76%andgrowthinhibitionregionsalinitybelow2%orabove20%．Therangeofhalophilictransitionregionsalinity0．68%～1．76%isshortanditspracticalsignificanceissmallsoitcanbenegligible．KeywordshighsalinitywastewateroptimalsalinityMonodequationgrowthkineticshalophilictransi-tionregion511784482012ZX07203-003-Ｒ052012－10－122012－10－221988～。[e-mail]*[e-mail]+、K+、Cl－SO2－4≥1%≥3．5%TDS12。3、456。7、89、10、1112、。、13。71415、16Diner170%5%COD85%59%。181920Ｒiffat2135℃6．2KgCOD/m3·dCOD80%。、“”“”22。Halomonassp．STSY-3。11．11．1．1CGMCCHalomonassp．STSY-3。1．1．2NaCl81．0gMgCl2·6H2O7．0gMgSO4·7H2O9．6gCaCl20．36gKCl2．0gNaH-CO30．06gNaBr0．026g5．0g10．0g1．0g1000mLpH7．2。1．21．2．123250mL100mL2%STSY-330℃150r/min2h1mL10mL600nmOD600。1．2．2ODOD50mL4000r/min10min105℃。OD600。1．2．3GB/T16285-20081mL4000r/min10min10μL10mL1000μL37℃15min。505nmAg/L=AA×g/L122．1STSY-3STSY-32%30℃150r/minOD600、12。1Fig．1Cellgrowthcurve1STSY-322h。2mg/L=766．34×OD600+45．6432Ｒ2=0．996。2．2STSY-3STSY-32%%NaCl30℃150r/minOD6003。6573102Fig．2Celldryweightstandardcurve3Fig．3Optimumsalinity3STSY-37%NaClOD6002．567%7%0、7%34%0%34%OD6000．470．06。2．3STSY-32．3．1Monodμ=μmax×SS+KS3μ=dCXdt×1C04C0g/LCXg/LSg/Lμh－1μmaxh－1KSg/L。Monod。。、STSY-32%30℃150r/minOD60024。4Fig．4Influenceofdifferentsubstancesoncellgrowth4STSY-3STSY-3。。2．3．2STSY-32%30℃150r/min、OD600OriginSμMonod5。5Fig．5Growthkineticscurveunderoptimumsalinity57%STSY-3μmax=2．257h－1KS=0．0082g/L75737μ=2．257×SS+0．00825STSY-3Monod。2．3．3STSY-3STSY-32%%NaCl30℃150r/minOD600Or-iginSμMonod1。1KS、μmaxTable1SaturatedconstantKSandmaximumspecificgrowthrateμmaxindifferentsalinity%KSg/Lμmaxh－100．13180．39520．03461．87550．02662．18570．00822．25790．01051．741150．01861．183200．05080．890250．14980．2831KS=0．0082g/Lμmax=2．257h－1。2%～9%KS9%～20%KS020%KS。STSY-37%100%75%、40%6。6Fig．6Divisionofhalophilictransitionregion675%～100%2%～9%40%～75%9%～15%40%≤2%≥20%。STSY-332%～9%、9%～20%0．68%～1．76%≤2%≥20%。0．68%～1．76%。31STSY-37%OD6002．567%7%0、7%34%0%34%OD6000．470．06。2STSY-3Monod。3STSY-3KS=0．0082g/Lμmax=2．257h－1μ=2．257×SS+0．00824STSY-3STSY-332%～9%、9%～20%0．68%～1．76%≤2%≥20%。0．68%～1．76%。1．．1999203104-106WenX．H．ZhanX．M．WangJ．L．etal．Ｒeviewofthebiologicaltreatmentofsalinitywastewater．Environmen-talScience1999203104-106inChinese2WoolardC．Ｒ．IrvineＲ．L．Ｒesponseofaperiodicallyoper-atedhalophilicbiofilmreactortochangesinsaltconcentra-tion．WaterScienceandTechnology199531141-508573103．．199824364-67YouZ．L．JiangZ．P．ZhuW．P．Directlyutilizeofseawateranditsenvironmentalanalysis．Water＆WastewaterEngineering199824364-67inChinese4．．199514473-78LiuH．B．Prospectanalysisofseawaterdesalinationanditsdirectutilizedinourcountry．OceanTechnology199514473-78inChinese5．-．19981637-10WenX．H．ZhanX．M．WangJ．L．etal．Ｒesearchonprecipitation-resinadsorptionofp-aminoazobenzenehydrochlorideproductionwastewater．EnvironmentalEngi-neering19981637-10inChinese6．．1993GuoY．H．Ｒesearchongroundwaterenvironmentalevolu-tionruleanditsrelationshipwithhumanactivityinHebeiPlain．BeijingDoctoralDissertationofChinaUniversityofGeosciences1993inChinese7．、．200832370-79WangW．LiuJ．J．ZhangG．F．Ｒesearchandanalysisoftreatmentofhighconcentrationandhighsaltwastewaterwithburningmethod．HeilongjiangEnvironmentalJournal200832370-79inChinese8．．200925121060-1063YeQ．LiZ．S．XuM．J．etal．Treatmentofhypersa-lineorganicwastewaterwithinteriormicro-electrolysisin-tensifiedbyultrasonicirridation．ＲesourceDevelopment＆Market200925121060-1063inChinese9SundarapandiyanS．ChandrasekarＲ．ＲamanaiahB．etal．Electrochemicaloxidationandreuseoftannerysalinewastewater．JournalofHazardousMaterials20101801-3197-20310．．201154726-729ZhaoX．G．LiW．ZhangZ．B．etal．Suppressionofliquidfilmru