高盐榨菜腌制废水处理的微生物系统构建研究周健

周　健,　吴绮桃,　龙腾锐,　王夏敏(重庆大学三峡库区生态环境教育部重点实验室,重庆400045)　　　:　针对高盐榨菜腌制废水生物处理过程中微生物系统难于构建的问题,研究了采用逐步驯化法构建高盐微生物处理系统的可行性及投加甜菜碱对高盐条件下生物脱氮系统建立的影响。试验结果表明:采用每次提高进水盐度为0.5%(以NaCl计)的逐步驯化方法,可建立能适应盐度为7%(以NaCl计)的高盐微生物处理系统,优势菌群为杆状嗜盐菌;在温度为25℃、DO为5mg/L、有机负荷为1.0kgCOD/(m3d)时,反应器对COD的去除率达到了97.4%;投加甜菜碱对高盐环境下硝化菌及反硝化菌的培养具有促进作用,缩短了生物硝化及反硝化系统的构建时间。　　:(7986)　　:　榨菜废水;　高盐;　微生物系统构建;　逐步驯化法:X703.1　　:A　　:1000-4602(2007)15-0017-05EstablishmentofMicrobiologicalSystemforTreatmentofMustardTuberWastewaterwithHighSalinityZHOUJian,　WUQi-tao,　LONGTeng-rui,　WANGXia-min(MOEKeyLaboratoryofThreeGorgesReservoirRegion’sEco-Environment,ChongqingUniversity,Chongqing400045,China)　　Abstract:　Inordertosolvethedifficultproblemsintheestablishmentofmicrobiologicalsystemduringthetreatmentofhigh-salinitymustardtuberwastewater,thefeasibilityofestablishinghigh-salinitymicrobiologicaltreatmentsystembyprogressivedomesticationmethodandtheeffectofbetaineadditionontheestablishmentofbiologicalnitrogenremovalsystemunderhigh-salinityconditionwereinvestigated.Theresultshowsthatundertheprogressivedomesticationprocess,inwhichinflowsalinityisperiodicallyincreasedby0.5%(calculatedasNaCl),ahigh-salinitymicrobiologicaltreatmentsystemcanbeestab-lishedtoenduresalinitylevelof7%(calculatedasNaCl),andthepredominantmicroorganismsarethebacilliformhalophiles.Underthetemperatureof25℃,theDOof5mg/Landtheorganicloadingof1.0kgCOD/(m3d),theCODremovalratereaches97.4%.Furthermore,theadditionofbetainecanac-celeratethecultivationofnitrobacteriaanddenitrifyingbacteriainhigh-salinityenvironmentandshortenthetimeofformingbiologicalnitrificationanddenitrificationsystemsunderhigh-salinitycondition.　　Keywords:　mustardtuberwastewater;　highsalinity;　establishmentofmicrobiologicalsystem;progressivedomesticationprocess　　,,,。3.4%(NaCl,)17第23卷　第15期2007年8月　　　　　　　　　　　　中国给水排水CHINAWATERWASTEWATER　　　　　　　　　　　　　Vo.l23No.15Aug.2007[1],1.0kgCOD/(kgVSSd),,CODBOD590%93%。[2],4.3%;(2.4%),24d。An2.5%3.4%[3],BOD590%。CharlesGlass、,7.7%,,[4]。(7%),,。1　试验材料及方法1.1　SBBR1。1　SBBRFig.1　SchematicdiagramofSBBRprocess　　SBBR7L,,30%;,、、、。1.2　,1。1　Tab.1　Qualityofwastewaterinexperiment/%COD/(mgL-1)BOD5/(mgL-1)NH+4-N/(mgL-1)TN/(mgL-1)0.5～725001500651801.3　1.3.1　SBBR,:pH7.0,1.0kgCOD/(m3d),DO5mg/L,25℃,45d;:(0.2h)—(11h)—(0.8h)。,,(0.5%),7%,COD。1.3.2　57%,0、0.1、0.5、1.0、1.5mmol/L,pH7.0,0.05kgCOD/(m3d),0.08kg/(m3d),DO5mg/L,25℃,45d;:(0.2h)—(11h)—(0.8h),NH+4-N、NO-2-NNO-3-N,。2　结果与讨论2.1　COD2。2　CODFig.2　DailyvariationofCODconcentrationduringdomestication18第23卷　第15期　　　　　　　　　　　　　　中国给水排水　　　　　　　　　　　　　　2,(0～1.5%)、(2%～3%)、(3.5%～4.5%)、(5%～5.5%)、(6%～6.5%)、7%,5、7、14、21、1421d,COD95%、(89%～93.2%)、(85.5%～94.8%)、(81.2%～83.9%)、(82.0%～87.0%)、97.4%。,,COD,,,COD。,,,,。,COD62mg/L,。,,。2。2　、Tab.2　Comparisonofbiofaciesbeforeandafterdomestication/%0,,0.5:;:,1:,;:1.5:,;:;:2:;:2.5:;:;:;:3:;:;:;:3.5:;:,;:;:4:、、,4.5:5,5.56()、()、(),、6.5()、()、(),、7()、()、,、　　,,。,,4%;2%～3.5%,4%;1%～2.5%,。BOD5[5],。,,,,,。,。,。5.5%,,,,。,5.5%,,:,,(),,,。0～6%,,5%,,,,,,,,,。6%～6.5%5%5.5%,6%,。,(Na+、K+)(、),,。,6%7%,,7%。,,,,,,。19周　健,等:高盐榨菜腌制废水处理的微生物系统构建研究第23卷　第15期2.2　3～5。3　NH+4-NFig.3　EffectofdosingbetaineonNH+4-Nremoval4　NO-2-NFig.4　EffectofdosingbetaineonNO-2-Nremoval5　NO-3-NFig.5　EffectofdosingbetaineonNO-3-Nremoval　　3～5,,NH+4-N,76.5～78.5mg/L17.7～22.6mg/L;NO-2-N,29.1mg/L;NO-3-N,2.3～3.0mg/L6.8～11.0mg/L。1.5mmol/L10d。7%,,,。,,,NO-2-NNO-3-N,。:,,[6],,,,,,。,,,,,,,。4,7d,,SBBR、,;,。,;,,。3　结论①　0.5%,7%,;25℃、DO5mg/L、1.0kgCOD/(m3d),COD97.4%。②　,。:[1]　,,.[J].,1998,17(9):8-10.(下转第25页)20第23卷　第15期　　　　　　　　　　　　　　中国给水排水　　　　　　　　　　　　。③　,SS、,,:,;。:[1]　,.[J].,2006,(2):1-4.[2]　,,,.[J].,2001,17(6):4-6.[3]　DasM.Characterizationofde-emulsificationcapabilitiesofaMicrococcusspecies[J].BioresourTechnol,2001,79(8):15-22.[4]　ParkSH,LeeJung-Hyun,KoSung-Hwan,etal.Demul-sificationofoil-in-wateremulsionsbyaerialsporesofaStreptomycessp[J].BiotechnolLett,2000,22(17):1389-1395.[5]　NadarajahN,SinghA,WardOP.Evaluationofamixedbacterialcultureforde-emulsificationofwater-in-petrole-umoilemulsions[J].WorldJMicrobiolBiotechnol,2002,18(5):435-440.[6]　,,,.[J].,1998,(6):22-23.[7]　JaniyaniKL,PurohitHJ,ShankerR,etal.De-emulsifi-cationofoil-in-wateremulsionsbyBacillussubtilis[J].WorldJMicrobiolBiotechnol,1994,10(5):452-456.[8]　,,,.TR-1[J].,2006,23(2):136-139.[9]　.[J].,2003,30(4):8-10.[10]　,,,.SZ36-1[J].,2001,18(2):136-138.:(1974-　),　,　,　,　,　。:[phone]E-mail:[e-mail]:2007-04-18(上接第20页)[2]　,,,.[J].,2002,22(6):546-550.[3]　AnL,GuGW.Thetreatmentofsalinewastewaterusingatwo-stagecontactoxidationmethod[J].WaterSciTechnol,1993,28(7):31-37.[4]　CharlesGlass.Denitrificationofhigh-nitrate,high-salinitywastewater[J].WaterRes,1999,33(1):223-229.[5]　,,.[J].,2005,31(11):57-60.[6]　,,,.Halomonassp.BYS-1[J].,2004,31(5):71-73.:(1964-　),　,　,　,　。:[phone]E-mail:[e-mail]:2007-04-1825黄翔峰,等:生物破乳剂对油田采出水处理系统的影响第23卷　第15期