镉胁迫对拟南芥中氨基酸及其衍生物的影响

29120101ENVIRONMENTALCHEMISTRYVo.l29,No.1January2010200959.*,Te:l041184379562,Emai:lchenjp@dicpaccn孙秀梅张纪秀张海军倪余文张青陈吉平*关亚风(,,116023).,,(Cd5Cd50),.:()(),;3();();,,(4).,,,,..,,.,[1,2],[3,4].,,[57].,,()[2,8].GCTOFMS,,.111(Ws,Wassilewskija,)445,10%(V/V),MS(08%),.,-80.:MS(pH62)0mol!l-1(Cd0),5mol!l-1(Cd5)50mol!l-1(Cd50);CdCl2!25H2O.12100200mg,,;//(),;/NN(MSTFA).GCTOFMS.Agilent6890GC/TimeofflightMS(Waters)/.:DB5MS(60m∀025m∀025mm,J&WScientific);:250;:802min,5!min-1180,3!min-1250,5!min-1300,20min;(He):1ml!min-1;06,l50#1.::EI;:70eV;:m/z30650.13,t(P005),6..SPSS.2629221MSTFA:,.,,.(GMD).60%,30(1),20,,,;,4;,.1GCTOFMSTable1ThequalitativeanalysisofaminoacidderivativesinA.thalianabyGCTOFMSNo./minNo./min11033C9H23NO2Si223311042162114C12H27O2NSi22731530521073C8H21O2NSi221911201742132C13H33NO2Si3319153763L1327C11H27NO2Si22611219118L2346C16H40N2O3Si43984L1431C9H23NO3Si224919L2357C14H33NO4Si3363162785L1471C12H29NO2Si22751270320L2498C13H32N2O3Si334816826L1530C12H29NO2Si22751298221L2529C16H41NO3Si44077L1533C10H25NO3Si2263222691C16H44N2Si4376174968L1544C11H25O2NSi22591302123/2721C14H36N2O2Si334891569C11H29O2NSi32911311824L2870C14H33NO4Si33631783310L1705C12H31NO3Si33211366325/2914C17H44N2O2Si44201830811L1711C12H31NO2Si330561367226L2999C15H38N2O2Si384612L1778C13H33O3NSi33351394527L3061C16H40N2O3Si442018842131874C12H31NO2Si330514334283175C17H35NOS335314L2022C13H33NO3Si33351448629L3202C18H46N2O2Si49181934715L2107C13H31O4NSi33491528130L3253C18H35NO3Si33971950222,(,319),,(1).20.,(Cd5Cd50),..,2().()(),;3();()..415;2.,[9]:,,1:27,;,,;,,,.1()(n=6,P005)Fig1AminoacidmetabolitelevelsincontrolA.thaliana.dataarenormalizedtotheinternalstandard(ribitol)(n=6)2Fig2MetabolicmapsofcentralmetabolismconcerningaminoacidsdisplayingthechangesinthemeanmetabolitelevelsinAthalianaundercadmiumstress,(4).,,[10].4pH,,,[11].2829330.,(Cd5Cd50).:()(),;3();();.,(4).[1]PalM,HorvathE,JandaTetal,PhysiologicalChangesandDefenseMechanismsIinducedbyCadmiumStressinMaize[J]JPlantNutrSoilSci,2006,169(2)#239246[2]SarryJE,KuhnL,DucruixCetal,TheEarlyResponsesofArabidopsisThalianaCellstoCadmiumExposureExploredbyProteinandMetaboliteProfilingAnalyses[J]Proteomics,2006,6(7)#21802198[3]YangX,FengY,HeZetal,MolecularMechanismsofHeavyMetalHyperaccumulationandPhytoremediation[J]JTraceElemMedBiol,2005,18(4)#339353[4],,,[J],2008,16(5)#13171327[5]ChiangHC,LoJC,YehKC,GenesAssociatedwithHeavyMetalToleranceandAccumulationinZn/CdHyperaccumulatorArabidopsisHaller:iAGenomicSurveywithcDNAMicroarray[J]EnvironSciTechnol,2006,40(21)#67926798[6]HerbetteS,TaconnatL,HugouvieuxVetal,GenomeWideTranscriptomeProfilingoftheEarlyCadmiumResponseofArabidopsisRootsandShoots[J]Biochimie,2006,88(11)#17511765[7]LessH,GaliliG,PrincipalTranscriptionalProgramsRegulatingPlantAminoAcidMetabolisminResponsetoAbioticStresses[J]PlantPhysiol,2008,147(1)#316330[8]SharmaSS,DietzKJ,TheSignificanceofAminoAcidsandAminoAcidDerivedMoleculesinPlantResponsesandAdaptationtoHeavyMetalStress[J]JExpBot,2006,57(4)#711726[9]SunRL,ZhouQX,SunFHetal,AntioxidativeDefenseandProline/PhytochelatinAccumulationinaNewlyDiscoveredCdHyperaccumulator,SolanumNigrumL[J]EnvironExpBot,2007,60(3)#468476[10]MiyashitaY,GoodAG,ContributionoftheGABAShunttoHypoxiaInducedAlanineAccumulationinRootsofArabidopsisThaliana[J].PlantCellPhysiol,2008,49(1)#92102[11]FaitA,YellinA,FrommH,GABAShuntDeficienciesandAccumulationofReactiveOxygenIntermediates:InsightfromArabidopsisMutants[J]FEBSLett,2005,579(2)#415420EFFECTSOFCADMIUMONAMINOACIDANDTHEIRDERIVATIVESINARABIDOPSISTHALIANASUNXiumeiZHANGJixiuZHANGHaijunNIYuwenZHANGQingCHENJipingGUANYafeng(DalianInstituteofChemicalPhysics,ChineseAcademyofSciences,Dalian,116023,China)ABSTRACTThispaperstudiedtheresponsesofaminoacidsandtheirderivativesinArabidopsisthalianaundercadmiumstressTheresultsindicatedtha,tthemajorityofthecompoundsdetectedwerefoundtoalterinresponsetocadmiumtreatmentThemetabolitelevelswithinthesamepathwaytendedtoalterintandemCoordinatedecreasesinthepoolsizesofanumberofaminoacids(Glu,Gln,Asp,Asn)derivedfromalphaketoglutarate,andoxaloacetatewereobserved,exceptProTheaminoacids(Ser,Gly)derivedfrom3phosphoglycerateincreasedTheaminoacids(Va,lLeu,Isoleu)derivedfrompyruvatealmostdidnotchangeAsmallgroupofaminecontainingmetabolites(Ala,4aminobutyricacid,tyramineandputrescine)withprotectivepropertiesappearedtobecoordinatelyincreasedAdditionally,wediscussedtheirresistantmechanismKeywords:Arabidopsisthaliana,cadmiumstress,aminoacidderivatives,stressresponses