共振散射光谱法测定部分水解聚丙烯酰胺的水解度凌绍明

　　　201129共振散射光谱法测定部分水解聚丙烯酰胺的水解度凌绍明(,　533000)　:在1.0mol/L醋酸介质中,部分水解聚丙烯酰胺(HPAM)与次氯酸钠溶液作用生成疏水性的氯酸胺微粒,该微粒体系在480nm处产生强的共振散射峰。HPAM的酰胺基团浓度在1.12～56.0mg/L范围内与480nm处共振散射光强度成线性关系,检出限为0.21mg/L。研究了共存物质对共振散射光谱法测定HPAM酰胺基浓度的影响,该方法的选择性较好,应用于样品中HPAM水解度的测定,结果满意。:部分水解聚丙烯酰胺;水解度;共振散射光谱法;次氯酸钠DETERMININGHYDROLYSISDEGREEOFHPAMPOLYMERSBYRESONANCESCATTERINGSPECTRALMETHODLingShaoming(DepertmentofChemistryandLifeScience,BaiseUniversity,Baise533000,China)Abstract:InthemediumofHAc,partiallyhydrolyzedpolyacrylamides(HPAM)reactedwithNaClOtoformammoniachloratenanoparticles,whichwerehydrophobicandhadastrongresonancescattering(RS)peakat480nm.TheRSintensityat480nm(I480nm)increasedlinearlytotheamidegroupcontentintherangeof1.12～56.0mg/L,andthedetectionlimitwas0.21mg/L.TheeffectofcoexistentsubstancesonthedeterminationofPAMwasstudied,anditshowedthisRSmethodhasgoodselectivity.ThehydrolysisdegreeofHPAMinsampleswasdetectedwiththismethod,andsatisfyingresultswereget.Keywords:partiallyhydrolyzedpolyacrylamides(HPAM);hydrolysisdegree;resonancescatteringspectralmethod;sodiumhypochlorite0　(HPAM)、,、、、。HPAM,;、、。HPAM,。,HPAM-[1]、-[2]、[3]、[4]、[5],。、,、[6-11],HPAM。,1.0mol/L,HPAM,,HPAM,,。1　1.1　仪器与试剂CaryEclipse(Varian,5nm,PMT400V);NaNo-ZS90Zeta(Malvern);PT-10pH(Sarto-rius)。(PAM,sigma,5×106,500mg/LPAM);1(HPAM1,3×106,28.6%,,);2(HPAM2,8×106,23.4%,,);3(HPAM3,0.6×106,15.8%,,);1.31g/L:239DOI:10.13205/j.hjgc.2011.s1.040　　　2011291.31g(≥10%),100mL(14d);5.0mol/L。1.2　测定原理1.0mol/L,HPAM,,HPAM480nm,ΔI480nm,。:1.3　实验方法5mL,HPAM,5.0mol/L0.5mL,,2min,0.75mL1.31g/L,2.5mL,30min。。480nmI480nmIb。ΔI480nm=I480nm-Ib。1.4　工作曲线的绘制1.3,(PAM)(HPAM),。1.5　水解度的测定1.3,(C0)(HPAM),ΔI480nm,C,HPAM。HD=1-C/C02　2.1　共振散射光谱,PAM,,PAM,,。1,480nm。480nm。2.2　实验条件的选择2.2.1　Cl2,Cl2PAM,。,,:c(HAc)=1.0mol/L;c(NaClO)=393.0mg/L;PAM(c(PAM)/(mg/L)):a,0.0;b,1.4;c,3.5;d,7.0;e,14.0;f,28.0;g,42.0。1　PAM-NaClO-HAc,,≥0.5mL,;≥0.9mL,ΔI480nm。5.0mol/L0.5mL。2.2.2　:c(HAc)=1.0mol/L;c(PAM)=28.0mg/L;T=25℃;t=30min2　ΔI480nm2。2:393.0mg/L,ΔI480nm。393.0mg/L。2.2.3　240　　　201129。ΔI480nm3。3,,ΔI480nm,30min,ΔI480nm。30min,,,30min。:c(HAc)=1.0mol/L;c(NaClO)=393.0mg/L;c(PAM)=28.0mg/L;T=25℃3　2.2.4　ΔI480nm,,40.0mg/LPAM,4。4,,ΔI480nm,18℃,ΔI480nm;25℃,ΔI480nm。:c(HAc)=1.0mol/L;c(NaClO)=393.0mg/L;c(PAM)=28.0mg/L;t=30min4　。,20～25℃。2.3　工作曲线,(PAM)ΔI480nm,c(PAM)ΔI480nm,。,PAMc(PAM)1.12～56.0mg/LΔI480nm,ΔI480nm=19.563c(PAM)+8.480,r=0.9975。11,3δ/K(δ,K),0.21mg/L。2.4　干扰实验,。PAMc(PAM)40.0mg/L,±10%,1。1,PAM,。1　(cCES/CMA)/%Na+,Cl-500+3.4K+,Cl-300+4.2Ca2+,Cl-40+5.8Mg2+,Cl-45+6.2Fe3+,Cl-15-8.0Cu2+,Cl-50+5.6Al3+,Cl-10-9.0Zn2+,Cl-80+7.4Ni2+,Cl-40+6.5Pb2+,Ac-30+7.1Co2+,Cl-50+6.7Mn2+,Cl-30+5.8Na+,CO32-25+4.9Na+,HCO3-100+5.2Na+,SO42-10+6.6Na+,NO3-500+4.52.5　样品的测定HPAM,,2。。2　/%/%RSD/%HPAM1HPAM2HPAM328.2,29.1,28.3,28.5,29.323.0,22.9,23.7,24.0,23.215.4,15.7,16.0,16.2,15.928.7±0.523.3±0.515.8±0.301.722.021.9328.5±0.423.4±0.415.8±0.3(268)241　　　201129,[5]。4.3　防护距离。1):GB/T13021-91《》;。80、90,21、、,,、。、,,,,。2),,,。,,。,,,,,,。5　,,,HJ2.2-2008,,、、,、、、、,,、。[1]HJ2.2-2008———[S].[2]U.S.EnvironmentalProtectionAgencyOfficeofAirQualityPlan-ningandStandardsEmissions,Monitoring,andAnalysisDivisionResearchTrianglePark.Screen3ModelUser'sGuide[M].NorthCarolina:U.S.EnvironmentalProtectionAgencyOfficeofAirQualityPlanningandStandardsEmissions,Monitoring,andAnaly-sisDivisionResearchTrianglePark,1995:43-45.[3]GBZ1-2010[S].[4],,.[J].,2008(20):6.[5].[J].,2010,43(5):52.　　100053　4　316E-mail　[e-mail]2011-05-16(241)[1],.-[J].,1997,14(1):73-76.[2]GB12005.6-89　[S].[3],,.HPAM[J].,2006,22(2):194-196.[4].[J].,2006,23(2):170-172.[5],,,.[J].,2004,23(4):59-60.[6]JIANGZL,ZHOUSM,LIANGAH,etal.ResonancescatteringeffectofrhodaminedyeassociationnanoparticlesanditsapplicationtorespectivedeterminationoftraceClO2andCl2[J].EnvironSciTechnol,2006(40):4286-4291.[7]JiangZL,LiaoXJ,DengGAP,etal.CatalyticeffectofnanogoidonCu(Ⅱ)-N2H4reactionanditsapplicationtoresonancescat-teringimmunoassay[J].AnalChem,2008(80):8681-8687.[8],,,.-[J].,2006,64(2):111-116.[9],,,.DNA[J].,2010,30(2):486-488.[10],,,.Pb2+[J].,2010,68(4):339-344.[11],,.[J].,2009,15(3):140-143.　　533000　E-mail　[e-mail]2010-12-31268