固定化细菌对低浓度重金属Pb2的吸附特征及影响因素郭平

　45　1()Vo.l45　No.1　20071JOURNALOFJILINUNIVERSITY(SCIENCEEDITION)Jan　2007Pb2+　1,1,1,2(1.,130012;2.,132101):采用固定化技术将从污水处理厂活性污泥中筛选的吸附重金属能力强的优势细菌固定,研究其对重金属离子Pb2+的吸附特征及其影响因素.结果表明,固定化细菌对Pb2+的吸附是动态过程,180min吸附达到平衡.随着Pb2+浓度的增加,固定化细菌与吸附量呈正相关;当吸附体系pH=6,离子强度为0.01mol/L时吸附量最大;环境温度对固定化细菌吸附Pb2+的影响包括两个阶段;吸附率随着吸附剂质量增加呈上升趋势,而单位质量吸附剂吸附Pb2+的量呈下降趋势;共存离子Cd2+降低了固定化细菌对Pb2+的吸附量.:固定化;吸附;Pb2+:X132　　:A　　:1671-5489(2007)01-0143-05TheCharacteristicandInfluencingFactorsofLowConcentrationPb2+AdsorptionontoImmobilizedBacteriaGUOPing1,GUOXiao-jing1,KANGChun-li1,ZHANGYi-jun2(1.CollegeofEnvironmentandResources,JilinUniversity,Changchun130012,China;2.JilinFuelAlcoholCompanyLimited,Jilin132101,JilinProvince,China)Abstract:ThecharacteristicandinfluencingfactorsoflowconcentrationPb2+adsorptionontotheimmobilizedbacteriafromtheactivatedsludgeinthesewagetreatmentplantwasstudied.TheresultsshowthattheadsorptionprocessofPb2+ontotheimmobilizedbacteriawasadynamicprocess,andachievedtheequilibriumstatewhthin180min.WiththeincreaseofthePb2+concentration,thenumberoftheimmobilizedbacteriawaspositivelyrelatedwiththeadsorptivecapacity.ThehighestuptakecapacitywasobtainedataninitialpHvalueof6andanintensityvalueof0.01mol/L.Theenvironmentaltemperature'sinfluenceontheadsorptivecapacityofPb2+includedtwosteps.Theadsorptionrateshowedanincreasingtrendwiththequalityoftheadsorbentraised,buttheadsorptivecapacityofPb2+byunitmassshowedadescendingtrend.TheconcomitantCd2+reducedtheadsorptivecapacityoftheimmobilizedbacteriaforPb2+.Keywords:immobilization;adsorption;Pb2+:2006-03-27.:　(1972～),,,,,,E-mail:[e-mail].:(1963～),,,,,,,E-mail:[e-mail].:(:205770141130702)(:419070200046).,,,.,,,,DOI:牨牥牨牫牬牨牫牤jcnkijdxblxb牪牥牥牱牥牨牥牫牥.,,.、、,,,.[1～3],(、、).、、、、,.,,,,.,,..,[4,5].,,、、、.,[6].,:(1),,;(2);(3),;(4);(5)pH.,Pb2+,.1　1.1　　　:、、、、、、、、,GR,CR,、BR,AR..:();HZQ-F160();PHS-3pH();WYX-9004().1.2　　　,、,.:,4%,2%,2～3mm(CA),24h,.,0.01mol/LNaNO3,180～200r/min[3].,,Pb2+,.2　2.1　、pH、　　pH=6.0,20℃,0.01mol/LPb2+,1.1,Pb2+,,5min730.34μg/g,84.72%;,180min.,3h.pH.20℃,0.01mol/L,pHPb2+2.2,144　　()　　　45　pH,Pb2+,pH=6,822.58μg/g(95.42%).pH,H+,H+Pb2+;,pH,()Ca2+H+,,、H+,,Pb2+[7];pH,H+,,pH,.pH=7,,Pb2+.Fig.1　AdsorptiondynamicalprocessFig.2　EffectofpHonadsorption　　,.pH=6.0,0.01mol/L.Pb2+(3),20℃,846.90μg/g(98.24%).5～20℃,Pb2+;20～40℃,Pb2+..4.pH=6.0,20℃,,,,:Y()=-0.34X()+1.00,　　R=0.9960.,().,Na+,Pb2+,Pb2+.Fig.3　EffectoftemperatureonadsorptionFig.4　Effectofionicstrengthonadsorption2.2　、　　pH=6.0,20℃,0.01mol/LPb2+,5.5,Pb2+,Pb2+,,:Y=159.91X+3.14,R=0.9974(R0.01(7-2)=0.7977).,Pb2+0.01.,,.,,,145　1　　　　　　　,:Pb2+　　　,[8]..pH=6.0,20℃,0.01mol/L,Pb2+6.6,,Pb2+,Y=0.2037ln(X)+1.2962,R=0.8588(R0.01(6-2)=0.8343);Pb2+,Y()=-4.96X()+1.38,R=0.9918(R0.01(6-2)=0.8343)(7).,Pb2+,,Pb2+.Fig.5　EffectofPb2+concentrationonadsorptionFig.6　Effectofadsorptionamountonadsorptionratio2.3　　　,,.Pb2+Cd2+,Pb2+,Cd2+.pH=6.0,20℃,0.01mol/L,8.,Cd2+,Pb2+,Cd2+,Pb2+,Cd2+,,(Y(Cd)=158.42X+154.07,R=0.9577(R0.01(6-2)=0.8343);Y(Cd)=89.53X()+30.66,R=0.9884(R0.01(6-2)=0.8343)).,Pb2+..Cd2+,,Cd2+,Pb2+,Pb2+.Fig.7　EffectofadsorbentamountonadsorptionprocessFig.8　EffectofCd2+concentrationonadsorption3　　　　Pb2+,,3h.pH,Pb2+,pH=6;Pb2+,20℃,Pb2+,20～40℃,Pb2+;,.Pb2+:20℃、146　　()　　　45　0.01mol/L.　　Pb2+Pb2+;,Pb2+,Pb2+.Cd2+,Pb2+,Cd2+.[1]　GUOPing,GUOPeng,MAXiao-fan,eta.lRelationshipbetweentheBiosorptionofPb2+andCd2+ontotheDominantDeadBacteriainNaturalWaterandInfluencingFactors[J].JournalofJilinUniversity(ScienceEdition),2004,42(3):471-474.(　,　,,.Pb2+Cd2+[J].(),2004,42(3):471-474.)[2]　NuhogluY,OguzE.RemovalofCopper(Ⅱ)fromAqueousSolutionbyBiosorptionontheConeBiomassofThujiaOrientalis[J].ProcessBiochemistry,2003,38:1627-1631.[3]　KANGChun-li,SUChun-yan,DONGDe-ming,eta.lStudiesonLeadandCadmiumAdsorptionbyExopolysaccharideofNaturalBiofilm[J].JournalofJilinUniversity(ScienceEdition),2005,43(1):121-125.(,,,.[J].(),2005,43(1):121-125.)[4]　YUXia,CHAILi-yuan,GANXue-ping.PresentStateandProspectofCellImmobilizationTechniqueandItsApplicationstoWastewaterTreatment[J].IndustrialWaterTreatment,2001,21(10):9-12.(　,,.[J].,2001,21(10):9-12.)[5]　WANGLi-ao,CUIZhi-qiang,QIANZong-qin,eta.lAdvancesinImmobilizedMicroorganismandItsApplicationsofWastewaterTreatment[J].JournalofChongqingUniversity(NaturalScienceEdition),2004,27(3):125-129.(,,,.[J].(),2004,27(3):125-129.)[6]　LIUHong-bin,LIUZhuan-nian,JINQi-ting.ApplicationofImmobilizedMicroorganisminWastewaterTreatment[J].TechniquesandEquipmentforEnvironmentalPollutionControl,2002,3(5):61-65.(,,.[J].,2002,3(5):61-65.)[7]　TUYong,ZHANGHong-ling.BiosorptionofHeavyMetalsbyImmobilizedActivatedSludge[J].EnvironmentalScienceandTechnology,2004,27(6):25-26.(　,.[J].,2004,27(6):25-26.)[8]　LIUHeng,WANGJian-long,WENXiang-hua.StudyonBiosorptionofHeavyMetalIon-leadbyYeast[J].ResearchofEnvironmentalSciences,2002,15(2):26-29.(　,,.[J].,2002,15(2):26-29.)(责任编辑:李桂英)147　1　　　　　　　,:Pb2+