管材和流速对供水管道生物膜形成的影响

4610201410JOUＲNALOFHAＲBININSTITUTEOFTECHNOLOGYVol.46No.10Oct．2014121212121212121．1500902．150090PCＲ－DGGE．、TOC、PE0.20.4m/s0.8m/sSphingomonasBacillus．TU991．21A0367－6234201410－0031－06InteractionofflowvelocitiesandpipematerialsonbiofilmformationZHUZebing12WUChenguang12ZHONGDan12YUANYixing12SHANLili12ZHANGJie12YUANYuan121．StateKeyLaboratoryofUrbanWaterＲesourceandEnvironmentHarbinInstituteofTechnology150090HarbinChina2．SchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnology150090HarbinChinaAbstractTheflowvelocitiesandpipematerialsmaybevariableindrinkingwaterdistributionsystemandtheirinteractiononthewaterqualityandbiofilmformationunderacertainchlorinelevelhavebeeninvestigated．HeterotrophicplatecountsanddenaturinggradientgelelectrophoresiswereemployedtoanalyzebiofilmformationandtheresultsshowedthatthechlorineTOCofbulkwatertotalbacteriacountsandmicrobialcommunitystructurewereallvariedinpipematerialswhileflowvelocitiesonlyaffectedthechlorineofbulkwaterandbiofilmbiomass．InthesametimePEpipesattachedsignificantlygreaterbiofilmbiomassthanstainlesssteel．Thebiofilmbiomassgrownunderflowvelocities0.2and0.4m/sweresignificantlygreaterthanthatunder0.8m/sbutthebiofilmgrowthbetween0.2and0.4m/swerenotsignificant．InthematurebiofilmmicrobialcommunitySphingomonassp．andBacillussp．weredominantinallbiofilmswhilethehydrophobicbacteriaweredifficulttogrowonthestainlesssteelpipeespeciallyunderhighflowvelocities．Keywordspipematerialsflowvelocitiesbiofilmmicrobialcommunitystructure2013－10－12．511081232014TS08．1984—1957—1938—[e-mail]．1．95%．．1025mg/L2、、3．、．、、、4．5．6．、47．、．11.161．3041.2m3PVC50L20PE10cm30mm、3mm．30412.5cm25mm、1mm．304PVC．PVC10mg/LNaClO13dPVC．、PVC70%38．NaClO30mg/L1130mg/L112、3、422h5．、．NaClOpH2h．1、2、3、51、210min4115．2、3、40.20.40.8m/s12h．22341、51．1.0mg/LHPC5CFU/mLpH7.321．234．!#$%&’()&*!+,-.&/!#$%&*#01!01#01%01&01’234.&/’()56789:;#&=?@&ABC11.27142135567798d223462HPC．．119d6HPCPCＲ－DGGE．710～30mL．1.3HPC1min1min330sＲ2AHPC．0.22μm10～100mLＲ2AHPC．·23·46Ｒ2A20℃10d．1.4DNAPCＲ-DGGEPowerSoilTMDNAIsolationKitMoBioLabaratoriesInc．CAUSADNA．PCＲDGGE8．PCＲ8F5’－AGAGTTTGATCCTGGCTCAG－3’、518Ｒ5’－ATTACCGCGGCTGCTGG－3’GC．NCBIBlastXMEGA5.2．1.5、、pH、、TOC、9DPDNN－－14－．1.6±SPSS13．0Fp＜0.05．22.112．1～15CFU/mLpH7.32．12h．、TOCp＜0.001．0.97mg/L12h0.20mg/LTOC2.14mg/L12h2.99～3.59mg/L．、TOC．PVCPVC、PEDOC10PVC1mg/LTOCTOCPEPVCPETOCPETOC0.39～0.54mg/LPETOC．0.97mg/L12h74%Gibbs111mg/LPVC0.720.86．PEPEp＜0.01PE10PE．1/m·s－1t/℃/NTUpH/μs·cm－1TOC/mg·L－1ρ/mg·L－1ρ/mg·L－10.221.99±3.180.62±0.227.38±0.06107.90±11.583.38±0.420.20±0.090.19±0.04PE0.421.44±3.150.65±0.257.39±0.06103.46±10.413.52±0.390.20±0.100.16±0.040.822.98±3.330.71±0.267.42±0.07102.15±10.003.59±0.400.20±0.080.15±0.050.221.81±3.190.60±0.197.37±0.06106.75±11.542.99±0.420.22±0.080.25±0.060.421.25±3.110.61±0.237.38±0.06107.78±10.053.13±0.400.21±0.080.22±0.060.822.61±3.230.67±0.237.41±0.06110.19±13.163.05±0.460.21±0.080.20±0.0718.30±3.090.46±0.137.32±0.06105.63±8.852.14±0.360.19±0.070.97±0.082FpHTOC0.150.400.302.8529.08＊＊＊0.2932.06＊＊＊39.07＊＊＊1.791.193.060.211.260.036.94＊＊0.54×0.7480.561.281.246.49＊＊＊0.089.24＊＊＊8.13＊＊＊PE1.000.641.561.361.220.014.44*0.550.790.561.510.390.500.043.140.10＊＊＊．p＜0.001＊＊．p＜0.01*．p＜0.05．·33·102．p＜0.0012．11d．．PE53811100～1800CFU/mL．p＞0.50.4m/s0.20.8m/s．39d8196480～580CFU/mL．PE．25．12h1～17d12．!!##$%&’(!##$)&’($%&#$%&’(!##$*&’($%&#$)&’($%&#$*&’(+####+###+##+###$+#%#)#,#*#+##+%#’()*’-+,-./*’0./01&23+122.23．．PEp＜0.00114dPE21d56PE7000～16000CFU/cm2982500～6000CFU/cm2．PVC、PEDOC10PE．0.8m/sPEp＜0.0010.4m/s0.20.4m/sp＞0.1．Lehtola130.3m/s0.5m/s13．．53～4m/s13．．!!#$%&’(!!#$)&’(#$#$*&’(!!#$*&’(#$#$%&’(#$#$)&’(+##+#+#$+#%#*#,#)#+##+%#%&’(’-)*+,-.(’/+#./012&34+032.3PCＲ－DGGE．4PE30%80%．4118NCBI5．45ProteobacteriaFirmicutes6α·43·46AlphaproteobacteriaγGammaproteobacteriaβBetaproteobacteria．Actinobacteria0.2m/sPE．Zhu81.20mg/Lαγββαγα．αSphingomonasechinoides10Bacilluscereus56PE．EPS14．15．．!!#$!#%!&’()*$+,!)$!#%!&’(-*$+,!.$!#%!&’(/*$+,00#$%&’#%!&’()*$+,00)$%&’#%!&’(-*$+,00.$%&’#%!&’(/*$+4PCＲ－DGGEPE0.8m/sγPseudomonadalesαＲhizobialesPE．PEβBurkholderialesγXanthomonadalesStenotrophomonasMicrobacteriumα0.2m/sPE．Bachmann16－17Pseudomonas、AcidovoraxMethylobacterium．AcidovoraxMethylobacterium/178．βＲhodocyclus0.2m/s18．54DGGE31、TOC、PEp＜0.001PEp＜0.05．2PE．．PE．PE567000～16000CFU/cm2·53·10982500～6000CFU/cm2．3α、γβ．．1SIMOESLCSIMOESMOLIVEIＲAＲetal．PotentialoftheadhesionofbacteriaisolatedfromdrinkingwatertomaterialsJ．JournalofBasicMicrobiology2007472174－183．2CHANDYJPANGLESML．DeterminationofnutrientslimitingbiofilmformationandthesubsequentimpactondisinfectantdecayJ．WaterＲesearch200135112677－2682．3SZEWZYKUSZEWZYKＲMANZWetal．MicrobiologicalsafetyofdrinkingwaterJ．AnnualＲeviewofMicrobiology20005481－127．4DOUTEＲELOISHAＲPEＲLBOXALLJB．InfluenceofhydraulicregimesonbacterialcommunitystructureandcompositioninanexperimentaldrinkingwaterdistributionsystemJ．WaterＲesearch2013472503－516．5TSAIYP．ImpactofflowvelocityonthedynamicbehaviourofbiofilmbacteriaJ．Biofouling2005215/6267－277．6SIMOESLCSIMOESM．BiofilmsindrinkingwaterproblemsandsolutionsJ．ＲscAdvances2013382520－