光合细菌固定化及其处理含油废水的研究毛雪慧

2009,28(7):1494-1499JournalofAgro-EnvironmentSciencePSBRSRhodobactersphaeroides。3。2%+2%CA。1000mL4mm1∶210g。6d、NH+4-N、PO3-480.1%、87.4%、96.3%。74.95%PSBRS35.31%50%。。X172A1672-2043(2009)07-1494-06121211.5106322.510632ImmobilizationofPhotosyntheticBacteriaforOilyWastewaterTreatmentMAOXue-hui1,XUMing-fang2,LIUHui1,ZENGLe2,XIAONa11.InstituteofHydrobiology,JinanUniversity,Guangzhou510632,China;2.BiotechnologyDepartment,JinanUniversity,Guangzhou510632,ChinaAbstractPhotosyntheticbacteriaRhodobactersphaeroidesPSBRSwasimmobilizedtotreatoilywastewater.ThreedifferentembeddingmaterialswerecomparedfortheoilremovaleffectoftheimmobilizedPSBRS.Theprocessforremovingoilwasoptimizedandthedegradationoffattyacidsinoilywastewaterwasalsoanalyzed.Thegelcomplexwith2%zeoliteand2%sodiumalginatewasselectedaftercomparisonforco-immobilization,withadiameterof4mmoftheimmobilizedgranuleandanembeddingratioof1∶2.After6daystreatmentwith10g·L-1granulesunderaerobicconditionsinthedarkness,theoil,NH+4-N,andPO3-4inthewastewaterwereremovedby80.1%,87.4%and96.3%.TheamountofoilremovedbyimmobilizedPSBRSwas50%higherthanthatbythesamebacteriawithoutimmobilization.Fattyacidswereal－solargelyremovedbyimmobilizedPSBRS.Thisworkisbeneficialtothescreeningandimmobilizationofmicroorganismsforefficienttreat－mentofoilywastewater.Keywordsimmobilization;photosyntheticbacteria;oilywastewater;ammonianitrogen;phosphate2008-10-111982—。[e-mail][e-mail][1]。C20H40O2~C16H30O2、[2]。。、、、、、、。、、、[3]。[4-5]。。[6-7]。Rhodobactersphaeroides287。11.1[7]g·LD-glucose4.0K2HPO40.015KH2PO40.006Na2HPO4·12H2O0.030NH4Cl0.117MgSO4·7H2O0.056CaCl20.010peptone0.150KNO30.0691000mL3300mg·L-1。1.2PSBRS[8]30℃、4000lx、4d5000r·min-115min225g·L-14℃。1.32%。1。10mL10cm2%CaCl22~4mm。2%CaCl224h。0.85%。1.41000mL1500mL3300mg·L-1。30℃6d、、。PSBRSPSBRS、、、PSBRS。1.51.5.1[9]1000mL65℃、、。=m1-m2/m1×100%m1gm2g。1.5.2[10]YSI550A。1.5.3PHILIPSXL－30ESEM。2.5%1h、。1.5.4[11]TRACE[ET]GC-MS-。DB-1730m×0.25mm×0.25μm99.999%1.2mL·min-1260℃60℃2min30℃·min-1145℃1.5℃·min-1250℃2min。EI70eV250℃220℃42~500。22.12.1.11。6d3PSBRSPVA65.3%。2A、B。[12]。。PVA。2C、D。[13]1Table1PercentageofgelscompositionPVA/%2101.5149520097。2E、FPVA。PVA[14]。PVA。48hPVA72hPVA[15]。PVA3。PVA、。2.1.26d3。3DO。[16][17]。DO1.23mg·L-170.4%。[18]。DO。1.23mg·L-1。1∶1、1∶2、1∶56d。2Figure2ConfigurationonthesurfaceofimmobilizedbeadsobservedunderSEMA.B.C.D.E.PVAF.PVAA.zeoliteswithnomicroorganism;B.zeoliteswithmicroorganism;C.glutinwithnomicroorganism;D.glutinwithmicroorganism;E.PVAwithnomicroorganism;F.PVAwithmicroorganismABCEFD149628741∶271.1%。1∶1[19]。1∶5。1∶2。2.25、10、15、20g·L-15。5.0~10.0g·L-1。10g·L-1。PSBRS、。5610g·L-1、80.1%、87.4%、96.3%。10g·L-1、48.6%、65.2%10g·L-1。、[20]。10g·L-1。2.31000mL10g149720097、10g30℃6、3、6dGC/MS。2C16H32O2、91215-C18H30O2、912-C18H32O2、9-C18H34O2C20H40O29-43.81%912-34.63%7.53%91215-7.44%。9-912-78%。3d9-912-13.29%12.18%、C17H34O29-C16H30O2C15H30O2。6d91215-。3dPSBRS9-912-9.53%7.11%C17H34O29-C16H30O2C15H30O2C3H8O3。6dPSBRS9-912-3.85%2.97%91215-、。。。β-CO2H2O[21]。[5]。29-912-、。3d912-。。6d。。26dPSBRSPSBRSPSBRS。3。。[22]。。。。312%+2%CA10%PVA+2%CA1.5%+2%CA。21.23mg·L-14mm1∶210g·L-1。6d、NH+4-N、PO3-480.1%、87.4%、96.3%。3。3Table3SummaryofoilremovalbyusingimmobilizedPSBRSandsuspendedPSBRS/%35.3174.959.622Table2ComparisonofvolatilefattyacidsremovalbymeansofimmobilizedPSBRSandsuspendedPSBRS/min/%/0d/3d/6d/3d/6d140.95C201.44－－－－231.519-C1843.8113.296.019.533.85332.43912-C1834.6312.184.717.112.97433.5591215-C187.441.76－0.95－526.15C170.08－－－－626.30C170.062.340.949.070.92735.12912-C170.251.380.557.610.69821.77C167.532.612.202.011.49921.979-C160.19－－－－1024.33C15－0.44－2.81－115.46C3－－－4.53－1498287[1],,.[J].,2001,1635-38.DONGXiao-dan,WANGChen-yu,WANGEn-de.Anexperimentalstudyonwastewaterprocessingintheinnsandrestaurantsbyusinganewagent[J].JournalofSafetyandEnvironment,2001,1635-38.[2],,,.[J].,2003911-14.HOUShi-bing,XUANXue-mei,JIAJin-ping,etal.Statusquoofre－searchandapplicationoftheoilywastewatertreatmenttechnology[J].ShanghaiChemicalIndustry,2003911-14.[3],,,.[J].,2007,28529-32.YANGYi-xun,JIANGYi,WANGYuan-yuan,etal.Researchprogressofoil-bearingwastewatertreatmenttechnique[J].JournalofChemicalIndustry&Engineering,2007,28529-32.[4]SasakiK,NoparatnarapornN,NagaiS.UseofphotosyntheticbacteriafortheproductionofSCPandchemicalsfromorganicwastes,inbioconver－sionofwastematerialstoindustrialproducts[M].NewYork,London,TokyoElsevierAppliedSciencePublishers,1991223-262.[5]HassanMA,ShiraiY,KusubayashiN,etal.Theproductionofpolyhy－droxyalkanoatefromanaerobicallytreatedpalmoilmilleffluentbyRhodobactersphaeroides[J].JournalofFermentationandBioengineer－ing,1997,835485-488.[6],.[J].,2005,25427-29.JIANGHao,GAOHong-mei.Immobilizationtechnologyofmicroorgan－ismandapplicationintreatmentofaquariumwater[J].ReservoirFish－eries,2005,25427-29.[7]NagadomiH,KitamuraT,WatanabeM,etal.SimultaneousremovalofchemicaloxygendemandCOD,phosphate,nitrateandH2Sinthesyn－theticsewagewastewaterusingporousceramicimmobilizedphotosyn－theticbacteria[J].BiotechnologyLetters,2000,221369-1374.[8]VanNielCB.Techniquesfortheenrichment,isolation,andmainte－nance