海水微生物菌群去除铵氮和亚硝酸氮研究闫海

闫　海　林毅雄(,,100085)孙建新(,524017)　　,NH+4-NNO-2-N。,,0.48g/L,3h5h,106mg/LNH+4-N49.9mg/LNO-2-N。　　　RemovalofammoniumandnitritebybacterialpopulationinseawaterYanHai　LinYixiong(StateKeyLaboratoryofEnvironmentalAquaticChemistry,ResearchCenterforEco-EnvironmentalSciences,ChineseAcademyofSciences,Beijing100085)SunJianxin(GuangdongLubaidaoBiotechnologyCompanyLtd.,Zhanjiang524017)Abstract　Removalofinorganicnitrogencontainingchemicalsbytheisolatedautotrophicandheterotrophicbac-terialpopulationswasinvestigated.ItwasshownthatboththegrowthandremovalofNH+4-NandNO-2-Nbyhet-erotrophicbacterialpopulationweremuchhigherthanthosebyautotrophicbacterialpopulation.Themixedautotrophicandheterotrophicbacteriawithhighactivityofnitrogenremovalwereobtained,andinitial106mg/LofNH+4-Nand49.9mg/LofNO-2-Ncouldberemovedat3and5hinthepresenceof0.48gdrycells/Lofmixedbacteriacul-tured,respectively.Keywords　autotrophicbacteria;heterotrophicbacteria;removalofnitrogen:2003-05-09;:2003-06-09:(1962～),,,,。1　　,,NH+4-NNO-2-N,,,[1]。,,。,NH+4-NNO-2-N[2],。Mike[3]Keller[4],。,。,,。,,,。,Ludwig[5](Thiosphaerapantotropha),。Robertson[6],。Van[7],,(T.pantotropha)(Nitrosomonaseu-ropaea)。411Vol.4,No.11200311TechniquesandEquipmentforEnvironmentalPollutionControlNov.2003。,(PSB),。PSB、[8],,PSB、NH+4-N,PSB[9]。NH+4-NNO-2-N,,。2　　2.1　(1):,,,(4℃)。(2):。[10],5.0g/L0.1g/LCaCl2,NaCl2g/L15g/L,K2HPO41.0g/L2.0g/L,[10]1.0mL/L。,2.0g/LNaHCO3,NH4ClNaNO2200mg/LNH+4-N150mg/LNO-2-N。,5.0g/L,200mg/LNH+4-N150mg/LNO-2-N。2molNaOH2molHCl,pH7.8。(3):,100mg/LNH+4-N50mg/LNO-2-N50mmol(NaCl15g/L;pH7.0),NH+4-NNO-2-N。2.2　(1)NH+4-NNO-2-N:[11]N-(1-)-[11],NH+4-NNO-2-N。,15g/LNaClNH+4-NNO-2-N,,。,(12000r/min,10min),,NH+4-NNO-2-N。(2):5g,10mL15g/LNaCl2h。0.5mL100mLNH+4-NNO-2-N。5d1mL,3。(3):,722680nm(OD680nm),。OD680nm,OD680nm。(4):124℃,。500mL,200mL。30℃,160r/min。3　3.1　NH+4-NNO-2-N　　[11]NH+4-N,0—2.1mg/L,NH+4-NOD420nm,NH+4-N(mg/L)=5.18×OD420nm-0.05(R2=0.9969)。N-(1-)-[11]NO-2-N,0—0.2mg/L,NO-2-NOD540nm,NO-2-N(mg/L)=0.444×OD540nm-0.003(R2=0.9984)。,NH+4-N,NO-2-N。15g/LNaCl,,NH+4-NNO-2-N,,NH+4-NNO-2-N。3.2　NH+4-NNO-2-N　　NH+4-N,1,NH+4-N,。5d4511　:,,2OD680nm1.6,,,5,OD680nm0.15(1)。NH+4-N,3200mg/LNH+4-N,,5NH+4-N175mg/L(1)。1　Fig.1　ThegrowthofautotrophicandheterotrophicbacteriaandtheremovalofNH+4_N2　Fig.2　ThegrowthofantotrophicandheterotrophicbacteriaandtheremovalofNO-2_N2,NO-2-N,NO-2-N,。5d,OD680nm0.0010.108,0.0010.521(2)。,5d,150.4mg/LNO-2-N60.5mg/L,,137.5mg/L(2)。,,,NH+4-NNO-2-NNO-2-NNO+3-N[1,3,4]。,、,[12],,NH+4-NNO-2-N。[13],,。,NH+4-NNO-2-N,(12)。NH+4-N2:NH+4-NNO+3-N,NH+4-N[3]。,NH+4-N[6],,[5]。[12],,。,,NH+4-NNO-2-N,。NH+4-NNO-2-N,,,[4]。3.3　NH+4-NNO-2-N　　NH+4-N,2g/LNaHCO35g/L,,NH+4-N100mg/L,。3,4d,OD680nm0.0010.85,2100mg/LNH+4-N0。,106mg/LNH+4-N49.9mg/LNO-2-N50mmol(1.5%NaCl),30℃,160r/min,。,0.48g/L,2hNH+4-N0,4645hNO-2-N0(4),NH+4-NNH+4-NNO-2-N。3　Fig.3　ThegrowthofmixedbacteriaandtheremovalofNH+4_N4　NH+4-NNO-2-NFig.4　TheactivitiesfortheremovalofNH+4_NandNO-2_Nbyculturedmixedbacteria,,BQ,,NH+4-NNO-2-N[1,2,9]。,,,,,5d,。,,2d(3)。,,,NH+4-NNO-2-N(4)。,,。4　　,NH+4-NNO-2-N,:(1)NH+4-NNO-2-N,。(2)NH+4-NNO-2-N。(3)NH+4-NNH+4-N,NO-2-N,。[1],..,2000,20(1):68—73[2],,,..,2000,19(6):339—341[3]MikeS.M.J.,SusanneL.,GerardM.,etal.Novelprinci-plesinthemicrobialconversionofnitrogencompounds.An-tonieVanLeeuwenhoek,1997,71:75—93[4]KellerJ.,YuanZ.,BlackallL.L.Integratingprocessengi-neeringandmicrobiologytoolstoadvanceactivatedsludgewastewatertreatmentresearchanddevelopment.ReviewsinEnvironmentalSci.&Biotechnol.,2002,1:83—97[5]LudwigW.,MittenhuberG.,FriedrichC.G.TransferofThiosphaerapantotrophatoParacoccusdenitrificans.Int.J.Syst.Bacteriol.,1993,43:363—367[6]RebertsonL.A.,KuenenJ.G.HeterotrophicnitrificationinThiosphaerapantotropha:Oxygenuptakeandenzymestudies.J.Gen.Microbiol.,1988,134:857—863[7]VanNielE.W.J.,ArtsP.A.M.,WesselinkB.J.,etal.Competitionbetweenheterotrophicandautotrophicnitrifiersforammoniainchemostatcultures.FEMSMicrobiol.Ecol.,1993,102:109—118[8],..,2001,21(1):23—36[9],,,..,2002,21(1):91—94[10],,..:,2001[11]..:,1998[12],,,..,2000,29(5):397—399[13],,,..,1999,27(3):251—254(责任编辑:郑晓梅)4711　: