海水养殖含氮废水处理的复合微生态制剂研制周鑫

微生物学通报APR20,2010,37(4):543−546MicrobiologyChina©2010byInstituteofMicrobiology,[e-mail]*通讯作者：Tel:[phone];:[e-mail]收稿日期：2009-11-24;接受日期：2010-01-13研究报告海水养殖含氮废水处理的复合微生态制剂研制周鑫王素英*宋霖霞(300134)摘要:目前应用于海水养殖废水修复的微生态制剂不仅存在菌株针对性、适应性差的缺点,而且很少考虑功能微生物之间的相互作用,因此应用效果难以令人满意。本实验针对海水养殖过程中的氮素循环,选择分离自海水养殖废水环境的亚硝化细菌ZW38、反硝化细菌ZL5和蛋白酶产生菌ZS7进行配伍实验,以混合培养体系中各种类型细菌的数量及氨态氮、亚硝态氮和蛋白质的含量变化为指标,发现3种功能细菌以3:2:1的比例混合,且使用量为109CFU/L时,彼此之间可相互促进生长,经过5d处理,海水养殖废水中的主要污染物质可溶性蛋白和氨态氮的去除率分别达到94.0%和77.5%。关键词:复合微生态制剂,氨态氮,可溶性蛋白DevelopingComplexProbioticsUsedinTreatingResolubleNitrogenWastewaterfromSeawaterCultureZHOUXinWANGSu-Ying*SONGLin-Xia(CollegeofBiotechnologyandFoodScience,TianjinKeyLaboratoryofFoodBiotechnology,Tianjin300134,China)Abstract:Atthepresenttime,probioticsusedinrecoveringseaculturewastewaterhassomedefects,suchasnogoodcounter,adaptationandrelationshipofthesestrains,sotheapplyingeffectisnotsatisfying.Inourexperiment,nitrosobacteriaZW38,denitrobacteriaZL5andsecretingproteinasestrainZS7,whichwereisolatedfromwastewaterofmarineculture,weremixedaccordingthenitrogencycleofseawaterculture,thenumberofvariousstrainandthecontentofNH4-N,NO2-Nweredetermined.TheresultsshowedthatthesestrainsgrowwelleachotherwhentheratioofZW38,ZL5andZS7is3:2:1,andthetotalinoculatingnumeris109CFU/L.After5dfortreating,thedecomposedrateofresolubleproteinandNH4-Nis94.0%and77.5%respectively.Keywords:Complexprobiotics,Ammonianitrogen,Resolubleprotein,,,[1],,,[2−3],,,[4−5]DOI:10.13344/j.microbiol.china.2010.04.008544微生物学通报2010,Vol.37,No.4材料和方法1.1材料::ZS7ZW38ZL51.2培养基[6][7][8]1.3实验方法1.3.1菌悬液的制备:ZS7ZW38ZL5,,4000r/min15min2−3,,1010CFU/mL1.3.2配伍实验:,1081091010CFU/L;ZW38ZL5ZS71:1:21:1:12:1:13:2:14:2:1,5d1.3.3制剂中菌株之间相互作用的研究:3,5d,24h31.3.4制剂对海水养殖废水的降解效果:3,8hFolin−[9];[10];(GB/T5009.33−1996)2结果与讨论2.1配伍实验结果,5d,,1表1不同接种量和菌种比例对氨氮降解的影响Table1EffectofinoculatednumberandratioofvariousstrainondecomposedamonianitrogenNo.Inoculatednumber(CFU/L)Inoculatedratio(ZW38:ZL5:ZS7)Contentofamonianitrogen(mg/L)11081:1:20.08221081:1:10.08131082:1:10.07941083:2:10.06351084:2:10.05961091:1:20.08071091:1:10.07481092:1:10.06991093:2:10.047101094:2:10.0501110101:1:20.0911210101:1:10.0721310102:1:10.0751410103:2:10.0561510104:2:10.0611,109CFU/L,3:2:1,,3:2:1(ZW38:ZL5:ZS7)2.2复合微生态制剂中不同菌株之间的相互关系,24h3,132,ZS7,,1.42×109CFU/L1.39×109CFU/L,(P0.05)ZL5(α=0.05),5d,ZL5,,,;:545×108CFU/LZW38(α=0.05),,ZW385d,(7.0×108CFU/L5.9×108CFU/L)(P0.05),图1混合接种各菌株的生长情况Fig.1Numberofvariousstraininmixedculture图2单独接种各菌株的生长情况Fig.2Numberofvariousstraininculturerespectively,,,,,N2ON2,,,2.3复合微生态制剂对海水养殖废水的降解效果实验,345图3接种后废水中可溶性蛋白含量变化Fig.3Variationofresoubleproteincontentinwastewaterafterinoculation图4接种后废水中氨氮含量变化Fig.4Variationofamonianitrogencontentinwastewaterafterinoculation图5亚硝酸盐含量变化Fig.5Variationofnitritecontentinwastewaterafterin-oculation546微生物学通报2010,Vol.37,No.4(1),ZS7,,,96h,5,40h,,88h,,,,ZL5,,88h,,,ZL5,,5d,94.0%77.5%3结论ZS7ZW38ZL5,,3:2:1(ZW38:ZL5:ZS7),109CFU/L,3,5d,94.0%77.5%,,3,,参考文献[1],,,..,1997,4(4):78−82.[2],..,2004,43(1):258−262.[3],..,2009,185(5):46−50.[4],,,..,2007,29(3):63−66.[5],,,..,2009,32(11):28−31.[6],,,..,2008,34(8):79−82.[7],,,..,2006,26(5):366−369.[8]TakayaN,Catalan-SakairiMAB,YasushiS,etal.Aerobicdenitrificationbacteriathatproducelowlevelsofnitrousoxide.AppliedandEnvironmentalMicrobiology,2003,68(6):3152−3157.[9].QB1805.3-1993,.:,1993.[10],..,2002,19(5):631−633.