含盐量对好氧颗粒污泥稳定性及微生物组成的影响李志华

:,,1976,,。＊(No.50708089、No.50621140001);(No.2006AA06Z328)。含盐量对好氧颗粒污泥稳定性及微生物组成的影响＊李志华　王立东　王晓昌(,,　710055)　　　(NaCl)。,(5.0%)(1.0%2.5%)。();,,(1)。,。,,。　　　　　　Effectofsalinityonthestabilityofaerobicgranularsludgeanditsmicrobialcomposition　LiZhihua,WangLidong,WangXiaochang.(SchoolofEnvironmentalandMunicipalEngineering,Xi'anUniversityofArchitectureandTechnology,KeyLaboratoryofNorthwestWaterResources,EnvironmentandEcology,MinistryofEducation,Xi'anShanxi710055)Abstract:　Wehavestudiedtheeffectofsalinityonthestabilityofaerobicgranularsludgeanditsmicrobialcom-position.Themicrobialcompositionofgranulesunderhighsalinity(5.0%)andlowsalinity(1.0%and2.5%)var-iedgreatly.SEMobservationshowedthatthemicroorganismsweremainlyrod-shapedandcoccus-shapedbacteriaun-derlowsalinity.Butalargeportionoffilamentousbacteriaappearedunderhighsalinity,andformedwhiteandblackfilamentousgranules,whichforashorttimedisplayedgoodsettleabilityandstrength.However,thesefilamentousgranuleslaterbecamelargerandlooser,andfinallydisintegratedinthereactor.Itappearedthattheproliferationoffil-amentousbacteriawasamainfactoraffectingthestabilityofaerobicgranularsludge.Keywords:　aerobicgranularsludge;salinity;filamentousbacteria;stability　　,。,,,。[1],,[2-5]。　　、、[6]。,NaCl1%[7]427。,,,。[7]427-433、[8]、SBR[9]—(MDAT-IAT)[10],。,、、[11]。1　1.1　试验及试验装置3(R1、R2R3),5cm,100cm,20,2.0L。。,CH3COONa258.3mg/L,189.00mg/L,NH4Cl189mg/L,KH2PO428.56mg/L,NaOH8.8mg/L,MgSO4·7H2O88.56mg/L。,1.70cm/s。、、、4,。·8·　　30　10　200810DOI:10.15985/j.cnki.1001-3865.2008.10.0142min,162min,10min,6min。45min。,50%,COD1.6kg/(m3·d)。1.2　含盐量变化　　(NaCl,),。18,30.5%;22,31.0%;30,R2R32.5%,R1;60,R35.0%,R1R2。2　2.1　好氧颗粒污泥颗粒化过程,R3,。,。,R3。1R3。19,(1(a));27,,(1(b))。,。1　Fig.1　Morphologyofgranulesindifferentstages2.2　不同含盐量下微生物的组成及特征,,、。,。278。,。,R1(1.0%)R2(2.5%)()(2(a));R3(5.0%),,,(SEM),(2(b)、(c)、(d))。2　(78)Fig.2　Microorganismcompositionofaerobicgranulesunderdifferentsalinity　　,(5.0%)、3,,()(3(a))。,,(3(b))。3　5.0%Fig.3　Morphologyofaerobicgranulesatdifferentperiods2.3　不同好氧颗粒污泥的特征及分析4(4　Fig.4　Settlingvelocityandporosityofdifferentgranules·9·　)。4,,80m/h;,。(110)(3(b)),。　　ZHENG[12],。,,;,,,。,(3(b))。2.4　好氧颗粒污泥稳定性的影响因素及分析,,。,,SS,。LIU[13],、、、。1,。,,、、pH,。,。,(3(a)),,。,(3(a))(3(b)),。1　Table1　Characteristicsoffilamentousbacteriaoccurredinaerobicgranularsludgesystemsunderdifferentcondition,COD(8kg/(m3·d))[14],COD(6kg/(m3·d))[12],[15],,pH[16],,[17],,-[18]3　　(1)(1.0%2.5%)();(5.0%),,。(2),,,。(3),。[1]　JIANGHelong,TAYJH,MASZENANAM,etal.Bacterialdiversityandfunctionofaerobicgranulesengineeredinase-quencingbatchreactorforphenoldegradation[J].Appl.Envi-ron.Microbiol.,2004,70(11):6767-6775.[2]　WANGShuguang,LIUXianwei,GONGWeixin,etal.Aerobicgranulationwithbrewerywastewaterinasequencingbatchre-actor[J].BioresourceTechnol.,2007,98:2142-2147.[3]　,,,.[J].,2006,28(9):703-706.[4]　,,.[J].,2006,28(10):788-791.[5]　,,,.[J].,2007,28(5):1026-1032.[6]　,,,.[J].,1999,20(3):104-106.[7]　KARGIF,DINCERAR.Salinewastewatertreatmentbyhalo-phile-supplementedactivatedsludgecultureinanaeratedrotatingbiodisccontactor[J].Enzyme.Microb.Technol.,1998,22(6).(下转第15页)·10·　　30　10　200810　　CRUZ-GUZMAN,SO3H/SiO2,。SO3H/SiO2SO3H。3　　(1)(SO3H/SiO2)。,SO3H/SiO2SO3H,SO3H/SiO2FTIRC—H、Si—O—SiSi—C,SiO2。　　(2)SO3H/SiO2(0.12gSO3H/SiO220mL1.4mg/L91%),,3h。。　　(3)SO3H/SiO2Langmuir,。SO3H/SiO2SO3H,—SO-3SO3H/SiO2。,。　　(4),SO3H/SiO2。[1]　NAVARROS,BARBAA,SEGURAJC,etal.Disappearanceofendosulfanresiduesinseawaterandsedimentunderlabora-toryconditions[J].PestManagementSci.,2000,56:849-854.[2]　COMBERSD.Abioticpersistenceofatrazineandsimazineinwater[J].Pestic.Sci.,1999,55:696-702.[3]　.[J].,2004,26(7):39.[4]　NAVARROS,VELAN,GIMENEZJMEA.Persistenceoffours-triazineherbicidesinriver,seaandgroundwatersamplesexposedtosunlightanddarknessunderlaboratoryconditions[J].Sci.TotalEnviron.,2004,329:87-97.[5]　HAYESMH.Adsorptionoftriazineherbicidesonsoilorganicmatter,includingashortreviewonsoilorganicmatterchemis-try[J].ResidueReviews,1970,32:131-174.[6]　WEBERJB.Mechanismsofadsorptionofs-triazinesbyclaycolloidsandfactorsaffectingplantavailability[J].ResidueRe-views,1970,32:93-130.[7]　CELISR,CORNEJOJ,HERMOSIMC,etal.Sorptionofat-razineandsimazinebymodelassociationsofsoilcolloids[J].SoilSci.Soc.Am.J.,1998,62(1):165-171.[8]　CRUZ-GUZMANM,CELISR,HERMOSINMC,etal.Ad-sorptionoftheherbicidesimazinebymontmorillonitemodifiedwithnaturalorganiccations[J].EnvironmentalScienceandTechnology,2004,38(1):180-186.[9]　SANNINOF,FILAZZOLAMT,VIOLANTEA,etal.Ad-sorption-desorptionofsimazineonmontmorillonitecoatedbyhydroxyaluminumspecies[J].EnvironmentalScienceandTechnology,1999,33(23):4221-4225.[10]　,,,.[J].,2007,29(3):165-167.[11]　SAGIVJ.Organizedmonolayersbyadsorption1.Formationandstructureofoleophobicmixedmonolayersonsolidsur-faces[J].J.Am.Chem.Soc.,1980,102(1):92-98.[12]　,,,.[J].,2006,28(9):41-44.[13]　,,,.[J].,2006,26(7):1226-1229.[14]　BOWMANBT,ADAMSJR,FENTONSW.Effectofwateruponmalathionadsorptionontofivemontmorillonitesystems[J].J.Agric.FoodChem.,1970,18(4):723-730.[15]　RUSSELLJD,MARIBELC,WHITEJL,etal.Modeofchemical-degradationofs-triazinesbymontmorillonite[J].Sci