杭州城区及其边缘地区湿地反硝化潜力评价王阳

　2010,22(8):159～161ActaAgriculturaeJiangxi王阳1,章明奎2＊　　:2010-05-25:(40771090);(R306011)。:(1963─),,,,。＊:。(1.,325006;2.,310029)　:以杭州湿地为例,对湿地反硝化强度及其特性进行了分析,结果表明:杭州市区及边缘地区的湿地由于受人类活动影响的不同及所处环境的差异,其湿地反硝化强度有较大的差异,反硝化强度在0.21～2.38mg/(kg·h)之间,城市化并没有减弱湿地的反硝化强度,城区湿地的反硝化平均强度高于城市边缘区,农田湿地反硝化强度低于其它湿地;湿地反硝化强度主要与有机质含量、全氮、微生物生物量碳和细菌数量呈正比,而受质地、pH和CaCO3含量等的影响较小,因此,湿地系统中有机碳含量可作为其反硝化潜力的判断指标。:杭州;湿地;反硝化作用;评价:X171.5　:A　:1001-8581(2010)08-0159-03DenitrificationPotentialofWetlandsinHangzhouCityandItsEdgeAreasWANGYang1,ZHANGMing-kui2＊(1.WenzhouVocationalCollegeofScienceandTechnology,Wenzhou325006,China;2.ZhejiangProvincialKeyLaboratoryofSubtropicalSoilandPlantNutrition,CollegeofNaturalResourceandEnvironmentalSciences,ZhejiangUniversity,Hangzhou310029,China)Abstract:Inthisstudy,wemeasuredthedenitrificationpotentialof37wetlandsintheurbanandedgeareasofHangzhoucity,southeastChina.Therelationshipsbetweenthedenitrificationpotentialandpropertiesofwetlandswerecharacterized.Theresultsshowedthatthedenitrificationpotentialsofdifferentwetlandswerediverseduetothegreatdisturbingbyhumananddifferentenviron-mentalconditions,theyrangedfrom0.21to2.38mg/(kg·h).Intensityofdenitrificationpotentialofwetlandsinurbanareawasgreaterthanthatinedgeareas,andthatoffarmwetlandswaslessthanthatofotherwetlands.Thefactthatthereweresignificantcor-relationsbetweendenitrificationpotentialandCcycle-relatedvariables(organicmatter,microbialbiomassC,numberofbacteria)suggestedthatorganicmatteraccumulationandCdynamicswerecriticalcontrollersofvariationindenitrificationpotentialofwetlandsintheseurbanandedgeareas.Keywords:Urban;Wetland;Denitrificationpotential;Evaluation　　,、。,,。,,。,,;,[1]。、,[2],,。,。NO3-NNO、N2O、N2,。NO3-N,,,。[3,4],。,,,。1　1.1　　,,,。,,,,,、。,1/3,、、、()。37,,、、、、、。DOI:10.19386/j.cnki.jxnyxb.2010.08.048:2、3、2、1、4、4、4、()4、6、7。0～5cm,2,,pH、、、CaCO3、N;,,,7d。1.2　　,、、N、pHCaCO3[5]。,。[6],,、3。,。,,()()。:41g50mL,10mL10g/L(),25℃48h,4000r/min20min,。、()。NO3-N,。─,ShimadzuTOC。[7],37℃4d。2　2.1　　37,。、CaCO345～369、8.5～160、0～53g/kg(1),258、50.211.7g/kg;pH6.2～8.4,7.4;N0.43～8.66g/kg,2.49g/kg。,,,。,、、,;pHCaCO3,pHCaCO3。,、PN,CaCO3,、pH(1)。1　(g/kg)289a224a87～36645～369pH7.0a7.3a6.3～7.86.2～8.4(g/kg)69.4a29.8b24.3～1608.5～53.6CaCO3(g/kg)4.83b18.9a0～270～53(g/kg)1.97a0.78b0.63～4.660.17～1.87N(g/kg)3.31a1.53b1.13～8.660.43～2.76　:5%,,。2.2　　0.21～2.38mg/(kg·h),0.84mg/(kg·h),11。,,。,、,(1.07±0.49)mg/(kg·h)(n=20),(0.58±0.31)mg/(kg·h)(n=17),1.84。,,,。1,、、0.89mg/(kg·h),0.41mg/(kg·h),。,,、,0.50mg/(kg·h),0.50mg/(kg·h)。1　2.3　　,、N、,0.9252＊＊、0.7899＊＊、0.9041＊＊、0.7491＊＊0.7120＊＊(n=37)。、pHCaCO3,0.4475、-0.0450-0.2703,,160　　　　　　　　　　　　　　　　　　　　　　　22(2)。,。2　3　NO3-N[8]。,,,。,(、),2:,,;,,O2,,。[9～11]:、,N。,,。,,。Groffman,、、、[9～11]。、、,。,,,,GroffmanTiedje,,。1,,。,,。,、、,,,。,NO3-N,,。(、、)。,,。4　,。0.21～2.38mg/(kg·h),0.84mg/(kg·h),11。,;。、、,、pHCaCO3。,。:[1]DekimpeCR,MorelJL.Urbansoilmanagement:agrowingcon-ern[J].SoilScience,2000,165:31～40.[2]GilliamJW.Riparianwetlandsandwaterquality[J].JournalofEnvironmentalQuality,1994,23:896～900.[3]EttemaCH,LowranceR,ColemanDC.Ripariansoilresponsetosurfacenitrogeninput:temporalchangesindenitrification,labileandmicrobialCandNpools,andbacterialandfungalrespiration[J].SoilBiologyandBiochemistry,1999,31:1609～1624.[4]HunterRG,FaulknerSP.Denitrificationpotentialsinrestoredandnaturalbottomlandhardwoodwetlants[J].SoilScienceSocie-tyofAmericanJournal,2001,65:1865～1872.[5].[M].:,1978:132～502.[6],,.[J].,2006,34(1):121～124.[7],.[M].:,1986:220～223.[8]LowranceR,VellidisG,HubbardRK.Denitrificationinare-storedriparianforestwetland[J].JournalofEnvironmentalquali-ty,1995,24:808～815.[9]GroffmanPM,TiedjeJM.Denitrificationinnorthtemperatefor-ests:spatialandtemporalpatternsatthelandscapeandseasonalscales[J].SoilBiologyandBiochemistry,1989,21:613～620.(下转第167页)161　8　　　　　　　　　　　　:。。3　“、”,。:。,850%,、、、,;,、,、[8],,,、,、;,,、。,、、[9],。[10],,,,,。,。、、。,、、、。,,,。、,。。,,。,,,,:“、”,,,“”,2008。:[1],.[J].,2007(6):24～25.[2],.[J].,2008(5):69～70.[3].[J].,2010,29(1):149～152.[4],,,.[J].,2010,29(2):91～93,132.[5].[J].,2006(4):1～4.[6].[J].,2008(4):35～36.[7],,,.[J].,2008,27(6):162～164.[8],,.[J].,2008(5):20～23.[9],,.[J].,2010,29(2):129～132.[10],,.PBL[J].,2009,21(8):203～204.(上接第161页)[10]WeierKL,DoranJW,PowerJF,etal.Denitrificationandthedinitrogen/nitrousoxideratioasaffectedbysoilwater,availablecarbonandnitrate[J].SoilScienceSocietyofAmericanJour-nal,1993,57:66～72.[11]ClementJC,PinayG,MarmonierP.Seasonaldynamicsofdeni-trificationalonghydrotoposequencesinthreedifferentriparianwetlands[J].JournalofEnvironmentalquality,2002,31:1025～1037.[12]MohnJ,SchurmannA,HagedornEetal.Increasedrateofdeni-trificationinnitrogen-treatedforestsoil[J].ForestEcologyManagement,2000,137:113～119.167　8　　　　　　　　　　: