杭州西湖底泥反硝化作用初探

36320155JournalofHydroecologyVol．36No．3May2015DOI10．15928/j．1674－3075．2015．03．0032015－02－13“·”2012ZX07101007-005311230015120849851308530。1989。[e-mail]。[e-mail]121212111．4300722．100049。201312201465313X1～X13。TN1．568～2．613mg/L1．117～2．848mg/LTN76%72%。0．132～1．350mg/g0．643～1．286mg/g0．015～5．092μg/g0．665～19．123μg/gP＜0．01P＞0．05。P＜0．01P＜0．05TOCP＜0．05。P＜0．01DOP＜0．05。。X820．6A1674－3075201503－0018－07。1986、1999－2003、2003。《2010》Ⅴ2014。Seitzingeretal2006。63%Berelsonetal1998Lietal2009。、。Goering＆Dug-dale199615NSrensen1978。N2OYuetal2008。。Abdelmagid＆Tabatabai1987Yuetal2008。O2、、pHKnowles1982。。。11．15、、、、3、80～300cm。。20131220146。13X1～X1311。1Fig．1WestLakesamplingsites1GPSTab．1GPSlocationofWestLakesamplingsitesGPSX11N30°13'42．5″E120°07'57．6″X22N30°13'42．5″E120°07'55．3″X31N30°14'01．5″E120°07'46．0″X42N30°14'06．6″E120°07'43．2″X51N30°14'31．1″E120°07'34．1″X62N30°14'31．5″E120°07'26．7″X71N30°14'01．3″E120°08'16．2″X82N30°13'55．7″E120°08'18．0″X9N30°14'45．5″E120°07'52．8″X10N30°15'31．9″E120°08'23．8″X11N30°15'09．3″E120°07'46．0″X12N30°14'37．7″E120°08'23．8″X13N30°14'08．3″E120°08'53．8″1．25～10cm4℃。－20℃。DO、TpHOＲION5-Star。1．3NH+4-NNO－2-NN-1--NO－3-NTNTP2002。1．41．4．1TOC。29．00～32．00mgElementarvarioTOC。1．4．2Nr5g350mL4mL、0．9mM24-DNP1mL、25mMKNO35mL225℃－20℃24h10mL、4MKCl5mL3mL、0．19MpH8．51mL15min520nm。μg/gAbdelmagid＆Tabatabai1987。1．4．3De15g50mL40mLKNO32．0g、3．0g、K2HPO40.27g、KH2PO40．09gpH7．53min28℃150r/min20h。TN。TNYuetal2008。1．5PearsonOne-wayANOVA。Pear-sonSPSS13．0P＜0．01P＜0．05P＞0．05。22．123。TP0.034～0．048mg/L0．032～0．056mg/L0.02～0．1mg/LⅠ－ⅡTN1.568～91201532．613mg/L1.117～2．848mg/L139TN2．0mg/L8Ⅴ。TN76%72%。NO－2NH+4DO。TNNO－3X1、X2、X3、X4X7、X8。。TOC。0．015～5．092μg/g0．665～19．123μg/g。0．132～1．350mg/g0．643～1．286mg/g。P＜0．01P＞0．05。2Fig．2WaterqualityparametervaluesateachWestLakesamplingsiteinwinterandsummer3Fig．3SedimentparametervaluesateachWestLakesamplingsiteinwinterandsummer02363201552．22NH+4P＜0．05P＜0．01。TOCP＜0．05。DOP＜0．05P＜0．01。2DeNrPearsonTab．2PearsoncorrelationcoefficientforeachvariablewithdenitrificationintensityDeandnitratereductaseactivityNrDeNrNH+4－0．20－0．61*－0．29－0．52NO－2－0．080．17－0．05－0．10NO－30．17－0．15－0．06－0．29TN0．09－0．26－0．17－0．34TP－0．11－0．010．080．320．090．75＊＊－0．150．38－0．28－0．090．040．08－0．560．80＊＊－0．400．42pH0．490．04－0．02－0．17DO0．63*－0．190．400．03TOC0．110．15－0．060．65*De/Nr0．67＊＊0．310．67＊＊0．30“*”P＜0．05“＊＊”P＜0．01。Note*significantcorrelationP＜0．05＊＊highlysignificantcorrelationP＜0．01．2．33。3。。X1X2X1X2。X3X4X3X4。33．120116201466、7、817～40℃617～36℃12、1、2－4～24℃1233Tab．3DistributionofsubmergedmacrophytesinthreesectionsofWestLake/%X113050X22－20X317035X425－X51210X62－－“－”。NoteThesymbol“－”representsnosubmergedplantdetectedatthesamplingsite．－4～18℃。612。0．015～5．092μg/g0.665～19．123μg/g2007Zhangetal2010aAbdelmagid＆Tabatabai1987122～126μg/g。。。Ｒevsbech1988－。。。3．2DODO。5～70℃1220153。DODOP＜0．052007DO。P＜0．05。2003。10℃Xieetal2003。7．2～9．2℃10℃23.5～25.5℃。。P＜0．01TOCP＜0．05TOCTOC。2003。TOC10g/kg20082011TOC54．486～742．065mg/kg。Yuetal2008、。3．3Panetal2011Zhangetal2010b。、、pH、DOSand-Jensenetal1985Eriksson＆Weisner1997Jonesetal2000Krner1999。TN、NH+4、TPTN、TPDaietal2012。235%65%230%22%10%。2。。。、、、、、、。．2002．M．．．2014．J．361160－66．．2003．D．．．2011．J．27292－95．．2007．J．383206－211．．2008．J．17181－85．．2007．J．382277－280．AbdelmagidHMTabatabaiMA．1987．Nitratereductaseac-tivityofsoilsJ．SoilBiologyandBiochemistry194421－427．BerelsonWMHeggieDLongmoreAetal．1998．BenthicNutrientＲecyclinginPortPhillipBayAustraliaJ．Estu-arineCoastalandShelfScience466917－934．DaiYJiaCLiangWetal．2012．EffectsofthesubmergedmacrophyteCeratophyllumdemersumL．onrestorationofaeutrophicwaterbodyanditsoptimalcoverageJ．Ecologi-calEngineering40113－116．ErikssonPGWeisnerSEB．1997．Nitrogenremovalinawastewaterreservoirtheimportanceofdenitrificationby2236320155epiphyticbiofilmsonsubmersedvegetationJ．JournalofEnvironmentalQuality263905－910．GoeringJJDugdaleVA．1966．Estimatesoftheratesofdeni-trificationinasubarcticlakeJ．LimnologyandOceanog-raphy111113－117．JonesJIEatonJWHardwickK．2000．Theinfluenceofper-iphytononboundarylayerconditionsapHmicroelectrodeinvestigationJ．AquaticBotany673191－206．KnowlesＲ．1982．DenitrificationJ．MicrobiologicalＲeviews46143－70．KrnerS．1999．NitrifyinganddenitrifyingbacteriainepiphyticcommunitiesofsubmergedmacrophytesinatreatedsewagechannelJ．ActaHydrochimicaetHydrobiologica27127－31．LiJLBaiJGaoHWetal．2009．Quantificationofdenitrif-yingbacteriaanddenitrificationprocessinsurfacesedimentatadjacentseaareaoftheYangtzeＲiverEstuaryinsummerJ．ChinaEnvironmentalScience297756－761．PanGYangBWangDetal．2011．In-lakealgalbloomre-movalandsubmergedvegetationrestorationusingmodifiedlocalsoilsJ．EcologicalEngineering372302－308．ＲevsbechNPNielsenLPChristensenPBetal．1988．Combinedoxygenandnitrousoxidemicrosensorfordenitri-ficationstudiesJ．AppliedandEnvironmentalMicrobiol-ogy5492245－2249．Sand-JensenKＲevsbechNPJrgensenBB．1985．Micro-profilesofoxygeninepiphytecommunitiesonsubmergedmacrophytesJ．MarineBiology89155－62．SeitzingerSHarrisonJABhlkeJKetal．2006．Denitrifi-cationacrosslandscapesandwaterscapesasynthesisJ．EcologicalApplications．1662064－2090．SrensenJ．1978．Denitrif