好氧脱氮微生物的混合培养条件耿金菊

2002,8(1):78~82ChinJApplEnvironBiol=ISSN1006-687X2002-02-25*耿金菊刘登如华兆哲陈坚**(214036),NH+4N2,NO-3.NaHCO3CH3COONa,0.25gL-1;(NH4)2SO40.2gL-1;pH7~10;30e;,30h98%,2.9gL-1.(NH4)2SO431.0%.6411;;CLCQ939.111.06+X172MIXEDCULTURECONDITIONSOFNOVELAEROBICDENITRIFYINGBACTERIA*GENGJinju,LIUDengru,HUAZhaozhe&CHENJian**(KeyLaboratoryofIndustrialBiotechnologyunderMinistryofEducation,SouthernYangtzeUniversity,Wuxi214036,China)AbstractAerobicdenitrifyingbacteriawereisolatedfromsoilandwater.Theammoniacouldbeaerobicallyox-idizedtonitrates,thenaerobicallyreducedtomolecularnitrogen.Thecultureconditionsofthemixeddenitrifyingbacteriawerestudied.ThemixtureofNaHCO3andCH3COONawasprovedtobetheoptimalcarbonsource,withtheirmassconcentrationbothbeing0.25gL-1.Theoptimalmassconcentrationofnitrogen(NH4)2SO4was0.2gL-1.pH7~10and30ewerethefavorableconditionsformixeddenitrifyingbacteria.Withtheseconditions,theammoniacouldberemovedbyover98%in30handthemaximumbiomassreached2.9gL-1.Thebiomasswasincreasedby31.0%usingfed-batchculturestrategy.Fig6,Tab4,Ref11Keywordsdenitrifyingbacterial;aerobicdenitrifying;mixedcultureCLCQ939.111.06+X172.,.:,N2.4~6wk,[1],,,.,:2001-05-23:2001-06-13*SupportedbytheTrans-centuryTalen-ttrainingProgramFoundationoftheMinistryofEducation**Correspondingauthor,,-.,,NO-3,;,OH-,pH;,,,.,[1~5],Pseudomonasspp.AlicaligenesfaecalisThiosphaerapantotropha[6],Robertson[6],Thiosphaerapantotropha/(),Poly-B-hydroxybuty-rate(PHB).Gupta[7]RBC(rotatingbiologicalcontactor)-,Thiosphaerapantotropha,-;.,[8],,,,,,,.,.,(NH4)2SO4,2L.11.11.1.1(aerobicden-itrifyingbacteria),.1.1.2(Q/gL-1)0.5,1(NH4)2SO420.2,NaCl1.0,FeSO40.4,K2HPO41.0,MgSO40.25,,Q(C)=0.5gL-1,Q(N)=0.2gL-1.1.21.2.110(U/%),50mL500mL,H=30e,200r/min.1.2.21Q(N)20.2gL-1,10(U/%),1.2L2L,30e,1L/(Lmin),400r/min,24h30h(NH4)2SO4Q(N)0.15gL-1.1.31.3.1[9].1.3.2[9].1.3.35mL,10000r/min10min,5mL0.1molL-1HCl,105e,1Q(DCW)/gL-12.22.12.1.1Q(C)Q(N)0.5gL-10.2gL-1,(1).CO2-3CO2,[10].(1).CH3COONa,CO2,.,.CaCO3,,[11],.,NaHCO3CH3COONa.2.1.22,0.5gL-1,,..1Tab1EffectofcarbonsourceongrowthandammoniaremovalofdenitrifyingbacteriaSortofcarbonsourceCaCO3NaHCO3CH3COONaNaHCO3+CH3COONa(1)CaCO3+CH3COONaQ(DCW)/gL-11.71.32.22.83.0Ammoniaremoval(r/%)(2)48.351.981.298.191.3Residualammonia(Q/gL-1)0.1370.1280.0490.0050.023Note:(1),NaHCO3CH3COONa1÷1()Thecarbonsourceconcentrationisthetotalcarbonmassconcentration,inwhichtheratioofNaHCO3andCH3COONais1÷1(Thesamebelow);(2)()Theammoniaremovalisnetammoniaremovalbymicrobes(Thesamebelow).791:2NaHCO3CH3COONa1Q0(C)2*Tab2EffectofconcentrationofNaHCO3andCH3COONaonthegrowthandammoniaremovalofdenitrifyingbacteriaQ0(C)/gL-10.10.20.30.50.8Q(DCW)/gL-11.82.32.42.92.6Ammoniaremoval(r/%)81.289.296.598.798.7Residualammonia(Q/gL-1)0.0500.0290.0080.0030.003*Q(N)=0.2gL-12.1.3(NH4)2SO4,Q0(N).3,,(NH4)2SO4,(NH4)2SO4,0.3gL-1,0.2gL-1,,0.2gL-1,(NH4)2SO4.2.22.2.1pHpH.pH7.2~8.07.0~7.5[10].(12),pH.pH7~10,6h,pH,,:pH(7~10),pH.pH.pH5,,;pH10,,,.3*Tab3EffectofQ0(N)ongrowthandammoniaremovalofdenitrifyingbacteriaQ0(N)/gL-10.050.10.20.30.40.5Q(DCW)/gL-11.391.982.872.901.821.73Ammoniaremoval(r/%)95.396.297.689.286.976.7Residualammonia(Q/gL-1)0.0010.0030.0040.0240.0400.092*Q(C)=0.5gL-11pHFig1EffectofinitialpHonDCW2pH(r/%)Fig2EffectofinitialpHonammoniaremoval(r/%)2.2.227e30e33e,(34)30e,,,,,;,80ChinJApplEnvironBiol8,.3Fig3EffectoftemperatureonDCW4(r/%)Fig4Effectoftemperatureonammoniaremoval(r/%)2.32.3.12.1.3,,.,,((NH4)2SO4).56.(Q(N))0.2gL-1,24h30h(NH4)2SO4Q(N)0.15gL-1.5,(24h),2.9gL-13.5gL-1,17.1%,(6).,(3.5gL-13.8gL-1),,31.0%.5Q(DCW)Fig5TimecourseofQ(DCW)duringfed-batchculture6(Q/gL-1)Fig6Timecourseofresidualammonia(Q/gL-1)duringfed-batchculture2.3.22L2L,(4),2L,,,15.4%..42LTab4Comparationofnomediumfedwithfed-batchcultureon2LfermentorModeofmediumfedNomediumfedFed-batchcultureQ(DCW)/(gL-1)3.33.9(r/%)Ammoniaremoval96.090.8(Q/gL-1)Residualammonia0.0080.037,NO-3.811:30h98%,2.9gL-1,3.9gL-1,.References1WilliamN.Denitrifyingbacteriumpreparationandmethod.USpatent,6,025,152.2000-02-152PaiSL,ChongNM,ChenCH.Potentialapplicationsofaerobicdenitrifyingbacteriaasbioagentsinwastewatertreatment.Biore-sourTechnol,1999,68(2):179~1853ZartD.HighrateofaerobicnitrificationanddenitrificationbyNi-trosomonaseutrophagrowninafermentorwithcompletebiomassretentioninthepresenceofgaseousNO2orNO.ArchMicrobiol,1998,169(4):282~2864RobertsonLA,KuenenJG.Aerobicdenitrification:acontroversyrevived.ArchMicrobiol,1984,139(4):351~3545RobertsonLA,KuenenJG.Aerobicdenitrification-oldwineinnewbottles?AntonievanLeeuwenhoek,1984,50(5~6):525~5446RobertsonLA,VanNielEWJ,Torremans,RobAM,KuenenJG.SimultaneousnitrificationanddenitrificationinaerobicchemostatculturesofThiosphaerapantotropha.ApplEnvironMi-crobiol,1988,54(1):12~18,287GuptaSK,RajaSM.Simultaneousnitrification-denitrificationinaroatingbiologicalcontactor.EnvironTech,1994,15:145~1538WangXP(),ChenJ(),HuaZZ(),LunSY().ChinJApplEnvironBiol(),1999,5(1):64~68956..3.:,1989.252~256,266~26910..:,1992.17~1911H#;..:,1960.4156()ISSN1006-687XCN51-1482/Q:62-15(),.,...56(1999).25,96,200116,.11.00,66.00.().199519961997199819992000(32.0044.0044.00