核黄素对偶氮染料生物降解的影响赵婧

赵婧1,2,蒋进元2,丁林2,周岳溪2*,梁杰11.,1000832.,100012:1(55?1)e2(UASB)-X-3B(RR2),(VB2).:(HRT)18h,Q(RR2)40~100mgPL,Q(CODCr)800~1200mgPL,Q(NaCl)30000~35000mgPL,UASB2~3kgP(m3#d),013~014kgP(kg#d),Q(VB2)5mgPL,RR2CODCr95%;UASB,.:;UASB;;RR2:X505:A:1001-6929(2009)10-1193-05EffectsofRiboflavinontheBiodegradationofAzoDyesZHAOJing1,2,JIANGJin-yuan2,DINGLin2,ZHOUYue-xi2,LIANGJie11.CollegeofChemistryandEnvironmentalEngineering,ChinaUniversityofMiningandTechnology,Beijing100083,China2.ResearchCenterofWaterPollutionControlTechnology,ChineseResearchAcademyofEnvironmentalSciences,Beijing100012,ChinaAbstract:TheeffectsofriboflavinonReactiveBrilliantRedX-3B(RR2)dye(areactiveazodye)removalfromhypersalinedyewastewaterandonthebiologicphaseofactivatedsludgewasstudiedwithathermophilicup-flowanaerobicsludgeblanket(UASB)reactorat(55?1)eandamesophilicthree-phaseaerobicbiologicalfluidizedbedreactorcombinedprocess.Experimentalresultsdemonstratethatriboflavincanimprovethedyeremovalinhypersalinewastewater.Whentheriboflavindosageisabout5mgPL,theremovalsofRR2andCODCrarebothmorethan95%(thehydraulicretentiontime(HRT)is18hours;influentRR2dye,CODCrandsalt(NaCl)are40-100mgPL,800-1,200mgPL,and30,000-35,000mgPL,respectively).TheorganicloadingratesofUASBandthethree-phaseaerobicbiologicalfluidizedbedreactorare2-3kgP(m3#d)and013-014kgP(kg#d),respectively.Thepredominantactivatedsludgemicrobeinthissystemisbacillussp.,andtheadditionofriboflavincausessmallercellvolumeandlesscellcontent.Keywords:riboflavin;UASB;three-phaseaerobicbiologicalfluidizedbed;RR2:2009-02-18:2009-03-17:(2008ZX07207-004):(1982-),,,[e-mail].*,(1964-),,,,,,zhouyuexi@2631net,35%[1].,[2].(80%),[3-4].,-.[5].X-3B(2,RR2)[6]..,RR2[7],RR2,,,,[8].,,,[9],.,-(RedoxMediator,RM),2210200910ResearchofEnvironmentalSciencesVol.22,No.10Oct.,2009DOI10.13198/j.res.2009.10.83.zhaoj.011,[10],.,56(GB4287)1992),.11.11.UASB,24L,(55?1)e,.,12L,,.1);2);3)UASB;4);5);6);7);8);9);10)ORP;11);12);13)1Fig.1Schematicoftheexperimentsetup1.2X-3B(RR2),,.(),100%.(VB2)-,,7,8--10-(D-1-).().1.3,Q(RR2)40~100mgPL,Q(NaCl)30000~35000mgPL,Q(VB2)10mgPL5mgPL.NH4Cl,KH2PO4N,P,Q(CODCr)BQ(N)BQ(P)200B5B1,NaHCO3,pH7~8.1.4,1:Q(SS)7171gPL,Q(VSS)5190gPL,Q(VSS)PQ(SS)017652,40%.UASB2~3kgP(m3#d),013~014kgP(kg#d).1.5[11](10mm@10mm@10mm).,;,,,;,,,.1.690:Q(CODCr)800~1000mgPL,Q(RR2)40~45mgPL,Q(NaCl)30000~35000mgPL,2LPh,HRT18h(,HRT12h,HRT6h).120,(VB2),Q(VB2)10mgPL,.2d1mgPL,130,Q(VB2)5mgPL,.182,Q(RR2)50mgPL,Q(RR2)50~55mgPL,7d,189Q(RR2)100mgPL,,.1.7Q(CODCr)Q(MLSS)[12].pHpH(pHS-25,);Q(RR2)(752N,);(FEIQUANTA200,).22.1,Q(VB2)10mgPL5mgPL,2,3,Q(VB2).2,3,90dRR280%,Q(RR2)10mgPL,119422CODCr80%,Q(CODCr)200mgPL,56(GB4287)1992)(/0).130d,.Q(VB2)P(mgPL):1)10;2)52RR2Fig.2ThechangeofRR2afteraddingriboflavin4,,UASBRR2Q(VB2)P(mgPL):1)10;2)53CODCrFig.3ThechangeofCODCrafteraddingriboflavin(538nm),()NN)),Q(RR2)5~10mgPL;,UASB538nm(370440nm2),Q(RR2)1~3mgPL,RR2()NN)),RR2.1);2)UASB;3)4Fig.4ThefullwavescanningunderaddingriboflavinornotCODCr,VB2,Q(CODCr),1CODCr,295%,Q(CODCr)40~50mgPL,.VB2RR2CODCr95%.[13-15],-,(5).152[16],5-Fig.5Proposedmechanismforredoxmediatorimprovedtheazodyemicrobialdecoloration-,.119510:,.Q(RR2)100mgPL,,Q(RR2)5mgPL,RR2CODCr.,-,RR2,,,,.2.2120160UASB,.2.2.1UASBUASB61,Q(MLSS)19~22gPL,.6(a),4000,,,,.16(b)2,,8000,,,.6UASBFig.6ThesludgemicrobialmorphologyintheUASB2.2.2717,4000,.,,,,,,,.7(4000)Fig.7Thesludgemicrobialmorphologyonthefillerafteraddingriboflavin(4000times)3a.UASB-,Q(RR2),Q(CODCr),Q(NaCl),Q(VB2)100,800~1200,30000~350005mgPL.12h,6h,;-,RR2CODCr80%95%.b.(VB2),VB2,,,,.,,.(References):[1].[N].,2003-06-23(6).[2],,.[J].,2007,32(11):89-92.[3]DOSSANTOSAB,CERVANTESFJ,VANLIERJB.Reviewpaperoncurrentechnologiesfordecolourisationoftextilewastewaters:perspectivesforanaerobicbiotechnology[J].BioresourceTechnology,2007,98:2369-2385.[4]DOSSANTOSAB,MADRIDMP,DEBOKFAM,etal.Thecontributionoffermentativebacteriaandmethanogenicarcheatoazodyereductionbythermophilicanaerobicconsortium[J].EnzymeandMicrobiolTechnology,2006,39:38-46.[5]MAASR,CHAUDHARIS.AdsorptionandbiologicaldecolourizationofazodyeReactiveRed2insemicontinuousanaerobicreactors[J].ProcessBiochemistry,2005,40:699-705.[6],,,.[J].,2007,20(4):102-105.[7],,,.X-3B[C]PP.:,2005:257-258.119622[8],,,.[J].,2007,27(2):174-178.[9],,,.MDAT-IAT[J].,2006,19(3):71-74.[10],,,.-[J].,2008,13(2):103-106.[11],,,.APDAT-IAT[J].,2006,6(6):52-55.[12].[M].4.:,2002.[13]HERNANDFZME,NEWMANDK.Extracellularelectrontransfer[J].CellularandMolecularLifeScience,2001,58(11):1562-1571.[14]DOSSANTOSAB,TRAVERSEJ,CERVANTESFJ,etal.Enhancingtheelectrontransfercapacityandsubsequentcolorremovalinbioreactorsbyapplyingthermophilicanaerobictreatmentandredoxmediators[J].BiotechnolandBioengin,2005,89:42-52.[15]FIELDJA,CERVANTESFJ,VANDERZEEFP,etal.Roleofquinonesinthebiodegradationofprioritypollutants:areview[J].WaterSciTechnol,2000,42:215-222.[16].[M].:,2007.(责任编辑:孔欣)119710: