化学解耦联剂对活性污泥工艺中剩余污泥的减量作用叶芬霞

:0253-2468(2004)03-0394-06　　　:X701.5　　　:A叶芬霞1,陈英旭2,冯孝善2　(1.,　315016;2.,　310029):,3,3′,4′,5-(TCS).,g0.5mg,TCS,30%.60d,COD,,SOUR.,60d,,,.,TCS,.:;;;3,3′,4′,5-(TCS);ReductionofexcesssludgeproductionbychemicaluncouplerinactivatedsludgeprocessYEFenxia1,CHENYingxu2,FENGXiaoshan2　(1.DepartmentofChemicalEngineering,NingboCollege,Ningbo　315016;2.DepartmentofEnvironmentalEngineering,ZhejiangUniversity,Hangzhou　310029)Abstract:3,3′,4′,5-tetrachlorosalicylanilide(TCS),ametabolicuncouplerwasaddedtocompletelymixedactivatedsludgeprocess.ThecontinuoustestshaveconfirmedthatTCSwasaneffectivechemicaluncouplerinreducingtheexcesssludgeproductionby30%whenitsspecif-icconcentrationwashigherthan0.5mg·g-1.ItwasalsorevealedthatCODremovalefficiencyandsludgesettleabilityfromthetreatedandcontrolsamplerswerecomparableandnotsignificantlydifferent,buteffluentnitrogenconcentrationwasaffectedadverselybythepresenceofTCSduringthe60doperation.SuchasludgegrowthreductionwasassociatedwiththeenhancementofmicrobialactivitiesintermsoftheSOUR.ThefilamentousbacteriaweremoreinthesludgefromsystemwithadditionofTCSthanthoseinthecontrolreactorafter60doperation.FewerprotozoaandmetazoangrowinthesludgefromthesystemwiththeadditionofTCSandtheiractivitiesweremuchlowerthancontrolsludge.TheseresultssuggestedthatitmightbepossibletoapplyTCSinactivatedsludgesystemstoreducetheexcesssludgeproductionbutitmightinfluencethepopulationinsludge.Keywords:activatedsludgeprocess;excesssludgereduction;protonphore;3,3′,4′,5-tetrachlorosalicylanilide(TCS);uncoupler:2003-04-11;:2003-11-15:(50201080):(1968—),,(),[e-mail],,.,,,..TeijsselTempest,,(energyspilling)(uncoupling),[1～3].,24320045　　　　　ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.3May,2004DOI:10.13671/j.hjkxxb.2004.03.005,.,,[4～12].,,.,3,3′,4′,5-(TCS),,TCSTCS,、.1　1.1　,1　Fig.1　Diagramofthetestapparatus1.13.5L,3.8L,5.6L.,,,,,,.1.2　.3,3′,4′,5-(TCS)(99.0%)Acros.1.3　2,.,,1.1　Table1　Componentsandconcentrationsofsyntheticmunicipalwastewater(mg·L-1)(mg·L-1)268MgSO466200CaCl26132KH2PO448.868(NH4)2SO41128FeSO40.3NaHCO380MnSO46　::CODCr=600mg·L-1,BOD5=280mg·L-1,=110mg·L-1,=60mg·L-11.4　2,,1,TCS,.70d.(HRT)4.63d,(FM)0.33kg·(kg·d)-1.10dTCS(0.4g·L-1)3,2mL,2.4mg.,60d1TCS(15mg).,TCS,TCS(),.26mg·L-1.(MLSS)3000mg·L-1,3953:,MLSS3000mg·L-1.25℃.1.4　:SVI;CODCr;MLSS(VSS);-.(SOUR):DO,300mL,.20min,MLVSS,SOUR,mg·(mg·d)-1,,.2　2.1　TCS260d2.2,22　Fig.2　Excesssludgeproductioninthe60dcontinuousoperation3　60dCODFig.3　CODremovalefficienciesoftwosystemsduringthe60dcontinuousoperation.,2.47g·d-1,TCS,1.73g·d-1,30.0%.TCS,60dTCS,,TCS,,..2.2　TCS2COD3.,TCSCOD.27d,,COD,,COD.TCS.2,4,6-[4]、2,4-[6]、[7,8]TCS[9,10].45.,TCS(NH+4-N)(TN).Low,,,,,.,396　　　　　　　　　　24　　　　　　4　60dNH+4-NFig.4　EffluentNH+4-Nconcentrationsofthetworeactorsduringthe60dcontinuousoperation5　60dTNFig.5　EffluentTNconcentrationsofthetworeactorsduringthe60dcontinuousoperation6　60dSVIFig.6　SVIvalueofthetwosystemsduringthe60-daycontinuousoperation7　60dSOURFig.7　SOURvalueofthetwosystemsduringthe60dcontinuousoperation.,TCS,TCS,,,.,-,.60d,SVI,SVI82.81,TCSSVI95.22,(6),Chen[9,10].2.3　TCSSOUR7.7,TCS,2.29mg·(mg·d)-12.93mg·(mg·d)-1,28.67%.May-hewStephenson[6]2,4-,.SOUR,,TCS,.,.2,3973:,、、.TCS,,(8).8　(a,b:;c,d:TCS)Fig.8　Thescanningelectronmicroscopicimageofthesludge(aandb:sludgefromcontrolsystem;candd:sludgefromsystemwithadditionofTCS)60d,2.,.TCS,.,,(8).TCS,,.Low,,[7,8].,TCS.3　,TCS,COD..SOUR,,.60d,,,,TCS,,..,,.398　　　　　　　　　　24:[1]　CookGM,RussellJB.EnergyspillingreactionsofStreptococcusbovisandresistanceofitsmembranetoprotonconductance[J].ApplEnvironMicrobiol,1994,60(6):1942—1948[2]　TempestW,NiejsselM.Physicologicalandenergeticaspectsofbacterialmetaboliteoverproduction[J].FEMSMicrobiolLett,1992,100(1):169—176[3]　RussellJB,CookGM.Energeticsofbacterialgrowth:balanceofanabolicandcatabolicreactions[J].MicrobiolReviews,1995,59(1):48—62[4]　StrandSE,HaremGN,StenselHD.Activatedsludgeyieldreductionusingchemicaluncouplers[J].WatEnvironRes,1999,71(4):454—458[5]　RiveranevaresJA,WymanJF,VonmindenDL,etal.2,6-ditert-butyl-4-nitrophenol(DBNP)apotentiallypowerfuluncouplerofoxidativephosphorylation[J].EnvironToxicolChem,1995,14(1):251—256[6]　MayhewM,StephensonT.Biomassyieldreduction:isbiochemicalmanipulationpossiblewithoutaffectingactivitysludgeprocessef-ficiency?[J].WatSciTechnol,1998,38(8-9):137—144[7]　LowEW,ChaseHA,MilnerMG,etal.Uncouplingofmetabolismtoreducebiomassproductionintheactivatedsludgeprocess[J].WatRes,2000,34(12):3204—3212[8]　LowEWandChaseHA.Theuseofchemicaluncouplersforreducingbiomassproductionduringbiodegradation[J].WatSciTech-nol,1998,37(4-5):399—402[9]　ChenGH,MoHK,LiuY.Utilizationofametabolicuncoupler,3,3',4',5-tetrachlorosalicylanilide(TCS)toreducesludgegrowthinactivatedsludgeculture[J].WatRes,2002,36(8):2077—2083[10]　ChenGH,MoHK,SabyS,etal.Minimizationofactivatedsludgeproductionbychemicallystimulatedenergyspilling[J].WatSciTechnol,2002,42(12):189—200[11]　OkeyRW,StenselHD.Uncouplersandactivatedsludge-theimpactonsynthesisandrespiration[J].ToxicolEnvironChem,1993,40(1):235—254[12]　LowEW,ChaseHA.Reducingproductionofexcessbiom