常见阴道炎实验诊断方法的应用与评价

:130031,,3:(bacterialvaginosis,BV)(vulvovaginalCandidiasis,VVC),,,BV,40%50%BV,,BV,,BVBV,Amsel[1],Nugent[2]1.Amsel:4:,,;;pH4.5;(whifftest)3,BV2.Nugent:,()()()3,,3,BV7;46;03,04+:0;1+1;2+14;3+530;4+302006(CDC)[3],BV,BV,DNApH(BVblue),,,,,1.:,1,,,60%70%2.:,,3.:,pH5.86.0,,363748h,1,85%90%7dCDC,(point2of2caretest,POCT),Osom(Genzyme)AffirmTMVP(BectonDickenson)83%,97%,(),,,227200612512ChinJGenPract,December2006,Vol5,No.12,VVCBV,,80%90%,,(),[3]1.:1ml10%KOH,1,,,VVC50%,10%KOH70%,10%KOH2.:,,3.:CHROMagarCDCVVC,,,10%20%,,,,,(),,,,,,1.:,80%90%,100%,4872h,,,,2.:(enzymeimmnunoassay,EIA)(directfluorescentantibody,DFA)EIA(LPS),LPS,DFA(MOMP)LPS,MOMPUnipath(ClearviewChlamydiakit),3.(nucleicacidamplificationtests,NAATs):NAATs,,,,RocheAmplicorPCR,AbbottLCx(LCR),BDProbeTecTMETDNA,99%Gen2ProbeAPTIMATM23SRNA[4],NAATsNAATs,NAATs,,,PCR,2(RT2PCR)327200612512ChinJGenPract,December2006,Vol5,No.12(),,,1.:95%,99%,[3]2.:,TM,,,NAATs,,()(UU),,UU,UU[5],,,UU41.2%,UU70%,,UU,,,,,PCR(FQ2PCR)UU,UU1.0106copy/g,;FQ2PCR1.0106copy/g,,,(HPV)(HSV)(CMV),PCRFQ2PCR,,,,;,,1AmselR,TottenPA,SpiegelCA,etal.Nonspecificvaginitisdiagnosticcriteriaandmicrobialandepidemiologicassociations.AmJMed,1983,74:14222.2NugentRP,KrohnMA,HillierSL.Reliabilityofdiagnosingbacterialvaginosisisimprovedbyastandardizedmethodofgramstaininterpretation.JClinMicrobiol,1991,29:2972301.3CentersforDiseaseControlandPrevention,WorkowskiKA,BermanSM.Sexuallytransmitteddiseasestreatmentguidelines,2006.MMWRRecommRep,2006,55(RR211):1294.4JohnsonRE,NewhallWJ,PappJR,etal.ScreeningteststodetectChlamydiatrachomatisandNeisseriagonorrhoeaeinfections22002.MMWRRecommRep,2002,51(RR215):1238.5,,,..,2005,21:6562658.(:2006209220)(:)20071,,,427200612512ChinJGenPract,December2006,Vol5,No.12