现代生物技术发展史上的重要事件

:1008-9632(2002)05-0057-04(扬州教育学院生物系,扬州225002),,,,,,21,21,,,,2070DNA,,1917,KarlEreky/0,1928,Flemming1943,6000,,1944,Avery(,DNA),MacleodMcCartyDNA1953,WatsonCrickDNA,,,,2050,Foley,Hable(polymavirus),,,,1956,SoberPeterson,1959B.JDavis(Palyaerylanidegelelectrophoresis)1959,Uchoa,,DNAKornberg,DNA1961,M.Nirenberg,DNA1964,,1964LittlefieldHATHAT1965,JacobMonod(operon)IwoffJacobMonod,DNA,mRNA1967,5DNA1970,T4DNA1969,DL-,1970,B-,1970,1971,,701972,KhoranatRNABergDNA,57195200210JOURNALOFBIOLOGYVol.19No.5Oct,2002)))1972,PotterBALB/C,MOPC(mineraloilplasmacytoma)1973,HerberBoyerStanleyCohenEcoRIpSC101,EcoRI,EcoRIDNADNA,DNA,/0,DNA,DNA,1973SchwaberCohen,1973,,,1980,2080,Anderson,,Joyner,1974JerneT,,,(Idiotype,ID)1975,KohlerMilstein,,,B1975,E.MSouthern(Blotting)DNA,Agarose,,,,,RNA,DNA-RNA,,DNA,,Southernblotting(Southern)1976,DNA1976,DNA1976,GenentechRobertSwansonHerbBoery1977,Genentech,,,1979,DNA,Genentech1982)))2070Imperial(ICI)Methlophilusmethylotrophus1979Imperial50000t,19803,(cecropin)ABcecropin,,,,,,1980616,DiamondChakrabarty,Chakrabarty/0,DNA,1980BotsteinWhiteSkolnickDavis4,1981,DNA1981,Behring,,KohlerMilsteinJerne,19841981(AIDS),,1985HIV,HIVDNA,199858195200210JOURNALOFBIOLOGYVol.19No.5Oct,20026DNA1982,DNA1983,Ti,1983,(SevereCombinedImmunodificientmine,SCIDmine)Bosma,CB-1716,T,TB,,-,Hu-SCID1985CetusMullisDNA,(polymerasechainreaction,PCR),Mullis19938,,PCR,PCR,PCR,PCR1986Beachy,(TMV)35S,TMV1986,Winter(reshapingantibody),-1986Dulbecco,,1987,(NIH)(DOE),19903@109bp,,1987Zimmermann1987Gordon(tPA),,19983,Ó,A1-,A-1988412,))),,,Chakrabarty1989,LuytjesA(ribonucleoproteincomplex,RNP),RNA1989,Huse(CombinantorialImmunoglobulinlibrary)1991WinterLerner(Phagedisplayantibody),,1989Ward(Singledomainantibody,SdAb)BeuggemanIgM(minilocus),4%L,IgM50Lg/ml,L1990,Rosenberg(TIL),TIL,neor,NIHDNA(recombinantDNAadvisorycommittee,RAC)1990914,1990,Hirataetal.Kaneetal.S.cerevisiaeH+-ATPase69KDVMA1,(Proteinsplicing)19917,/B0,,,199355,561991,White(antitrypsin,ATT),,5,4,,4ATT,1~35g/L,250~800L,1991,59195200210JOURNALOFBIOLOGYVol.19No.5Oct,2002,126141993,,JespersL.S:(Epitopeguidingselection,EGS,Epitopeimprintedselection,EIS),()()()()EGS1993,WolffDNA,2DNA[(geneticimmunity)]19941995,,DNA,DNA1994,Perler,Xuetal.(thebranchedintermediate)1994,GilmanRodbellG1995,LewisNusslein-volhardWieschaus2040~701996DohertyZinkernagelMHC,,1997223,Wilmut,))),,19981,19987,,,,,1998,Rohl32001656,,,,2001126,11)))328,2001529(HaSNPV),HaSNPV,,HaSNPV,/0,,2002215,/0,/0,,,2002,:,/0,MeselsonStah1(1958)DNA,Crick1954))),YanofskyBrener(1961),2020,20607080,,,,,,,60195200210JOURNALOFBIOLOGYVol.19No.5Oct,2002