人促卵泡激素时间分辨荧光免疫分析检测试剂的研制Ξ

X1,2,2,1,1(11,510515;21,510663):(TRFIA)(hFSH)hFSH2TRFIA,hFSH2TRFIA1256UöL,0.08UöL,2.6%4.2%,6.1%8.6%,(hLH)(hTSH)(hCG)0.14%,0.08%0.01%30,0.993,:(hFSH);(TRFIA);:R446161:A:167127414(2006)062036203Timed-resolvedFluoroimmunoassayKitforDetectionofHumanFollicleStimulatingHormoneWUYing2song1,DONGZhi2ning2,TANGYong2ping2,XUWei2wen1,LIMing1(1.CollegeofBiotechnology,NanfangMedicalUniversity,GuangdongGuangzhou510515,China;21GuangzhouDaruiAntibodyCO.LTD,GuangdongGuangzhou510663,China)Abstract:ObjectiveToprepareakitfordetectionofhumanfolliclestimulatinghormone(hFSH)inserumbytime2resolvedfluoroimmunoassay(TRFIA).MethodsTwoanti2hFSHmonoclonalantibodieswereusedtodevelopthesandwichTRFIAkitfordetectionofhFSHinserum.ResultsThemeasurerangeofdevelopedkitwas1256UöL.Itsassaysensitivitywas0.08UöL.Thecrossreactivityratewas0.14%,0.08%and0.01%withhumanluteinizinghormone,humanthyroidstimulatinghormoneandhumanchorionicgonadotropin,respectively.Theintra2andinter2assaycoefficientsofvariationwere2.6%4.2%and6.18.5%,respectively.Thecorrelationcoefficientofbloodsamplesdetectionresultsbetweenhome2madeandcommerciallyavailableDELFIAFSHkits(PerKinElmer)was0.993.ConclusionThedevelopedhFSH2TRFIAkitinthisstudyisavaluabletestforclinicalapplicationwithbettersensitivity,specificityandaccuracy.Keywords:humanfolliclestimulatinghormone(hFSH);timed2resolvedfluoroimmunoassay(TRFIA);detection(humanfolliclestimulatinghormone,hFSH),hFSHKlinefelterTurner;,,,hFSH,hFSH,[1][2][3][4](timed2resolvedfluoroimmunoassay,TRFIA),,,PerKinElmerFSH2DELFIA,,hFSH2TRFIA,,11.1FSHMedix,FSHBiodesign;96labsystem;SephadexG250Pharmacia;FSH;Eu3+FSH2DELFIAPerKinElmer;Auto2Delfia123530FSH1.211211:50mmolöLpH9.63Lgöml,200Ll96,4,,(2gödlBSA),250Ll,4,,,-201.212Eu3+:0.5mgMillipore,8000römin56min(50mmolöL,Na2CO3,pH9.0)6200Ll63200611216JModLabMed,November2006,Vol.21,No.6X:(2004Zl2E4021):(1974-),,,,,E2mail:[e-mail]50Lg,251.213Eu3+:SephadexG-50(1cm30cm),(0.9gödlNaCl50mmolöLTris-HCl),(1mlö),(A280nm),,,Eu3+(IgEu3+)8.7,95.8%1.214:0.2gödlBSA,0.1gödlNaN3,50mmolöLTris2HCl(pH7.8)hFSH0,1,4,16,64,256UöL,1ml,41.215:25Ll,200Ll,3h,4,1÷75200Llö,30min,6,200Ll5minAuto2Delfia1235AutoDELFIA1235TRFIA22.1,(Log2Logit),2(1),tP0.05,;FSH,0.941.07(1)2r=0.9989,A:2;B:21hFSH2TRFIA21hFSH2TRFIA(n=8)(UöL)(UöL)ABCDEF01416642560(00.09)0.94(0.911.03)4.07(3.974.33)16.8(16.217.4)65.7(60.569.5)269(245277)-0.94(0.911.03)1.01(0.991.08)1.05(1.011.09)1.03(0.951.09)1.05(0.961.08)2.21256UöL,1500UöL,87.3%,2.3(A)202,0.08UöLPerKinElmerFSH2DELFIA0.05UöL,2.4hFSH(IIIIII,5,15,50UöL),,82,10%,2(xq2s,UöL)(n=8)xqsCV%xqsCV%xqsCV%(,n=38)xqsCV%É(040301)Ê(040603)Ë(040902)515506.600.1717.00.6556.32.362.63.84.26.220.2516.70.4552.52.844.02.75.46.820.1916.80.6256.81.762.83.73.16.540.5616.50.5955.43.388.57.66.1:2.5250UöL(hLH)300mUöL(hFSH)2000UöL73200611216JModLabMed,November2006,Vol.21,No.6(hCG)0.35UöL,0.24mUöL0.10UöL,0.14%,0.08%0.01%2.630(Y)FSH2DELFIA(X),,Y=0.963X+0.553,r=0.993,P0.0013TRFIA,,,,[5],,,,TRFIAhFSH,hFSH2TRFIA0.08UöL,1256UöL,DPC(:0.1170UöL)(0.3200UöL)FSHPerKinElmerPRL2DELFIA,3FSH2TRFIA10%,41y377d,TRFIAHOOK,hFSH,01400UöLhFSH1400UöL,,1400UöL1÷103hFSH2TRFIAFSH2DELFIAhFSH2TRFIAFSH2DELFIA(UöL)(Ll)(UöL)(%)3h40min0.08251256CV7.2,41y3h40min0.05251256CV5.5,41y,FSH:[1],.ELISALHFSH[J].,2002,17(2):1516.[2],,.FSHLH[J].,1997,22(6):425426.[3],,.FSHLH[J].,1996,7(4):210.[4],,.E22FSHLH[J].,2005,18(5):407408.[5],,.[J].,2004,4(3):340343.:2006207203ÊX,,,(,641100):API20STREPÊ,API20STREP27h,612h,API20STREP:46414534641003,ÊBACTEC9050,BD,,API20STREP:Ê;;API20STREP:R37811+2R44615:A:167127414(2006)062038202,Ê[1],2005204,43,122,31,5,4Ê,3,183200611216JModLabMed,November2006,Vol.21,No.6X:(1967-),,,,,E2mail:[e-mail].