小鼠肝动脉重建对长时间冷保存移植肝存活率的影响

DOI:103969/jissn16747445201003002:(2009B030801012):510080,:,Emai:lwgdgd@163com王国栋!(hepaticarterialreconstruction,HAR)C57BL/668,(coldpreservationtime,CPT)1hCPT4hCPT8hCPT16hCPT16h+HAR()4∀UW()(),HAR5,,CPT1h4h8h12d7/710/109/9CPT16h1,36h,10%(1/10),CPT16h+HAR90%(9/10),,(P001)CPT1h4h,CPT8h,CRT16h,CPT16h+HAR,,HAR!;;;;EffectofhepaticarterialreconstructioninmiceonthesurvivalrateoflivergraftsfollowinglongtermcoldpreservationinjuryWANGGuodongDepartmentofTransplantationSurgery,FirstAffiliatedHospitalofSunYatsenUniversity,Guangzhou510080,ChinaCorrespondingauthor:WANGGuodong,Email:wgdgd@163comAbstract!ObjectiveTostudytheeffectofhepaticarterialreconstruction(HAR)onthesurvivalrateoflivergraftsfollowinglongtermcoldpreservationinjuryMethodsSyngeneicmaleC57BL/6mice(n=68)weredividedinto5groups:CPT1,4,8,16handCPT16h+HARgroupLiverperfusionwasflushedintoportalveinwith4∀UWsolutionandthegraftswerepreservedin4∀UWsolutionOrthotopiclivertransplantation(OLT)wasperformedwithacombinationofsuture(suprahepaticvenacava)andcuffanastomosistechnique(infrahepaticvenacavaandportalvein)ThebileductwasreconnectedthroughtheintraluminalstentThemethodsforHARinmiceincludeendtosideanastomosisofabdominalaortawithhepaticarteryfromdonorliverstoabdominalaortaintherecipientRecipientsurvivalwasfollowedupandhepatocellularinjurywasevaluatedbyhistologyHepatocytereplicationwasexaminedbyimmunohistochemistryResultsSurvivalrateonpostoperativeday12inCPT1,4,8hgroupwas7/7,10/10,9/9,respectivelyRecipients(9of10)inCPT16hgroupdiedwithin36haftertransplantation,whereasonly10%(1/10)ofmiceremainedaliveonday12aftertransplantationAllanimalsbutoneinCPT16h+HARgroupsurvived12dafterarterialreconstructionTherewassignificantdifferenceinsurvivalratebetweenthetwogroups(P001)HistologyshowedmildinjuryingraftsofCPT1and4hgroupsMoresevereinjuryandobvioushepatocytereplicationwasobservedinCPT8hgroupcomparedwiththeCPT1and4hgroupsHistologyexaminationin16hCPTgroup1352010513OrganTransplant,May2010,Vo.l1,No.3showedextensivehepatocytevacuolardegenerationandareasofnecrosiswithoutincreasedhepatocytereplicationLivergraftsinCPT16h+HARgroupshowedmildsinusoidalcongestion,vacuolardegeneration,hepatocytenecrosisandactivehepatocytereplicationConclusionHARcanimprovethesurvivalrateoflivergraftswithseverelongtermcoldpreservationinjuryKeywords!Mouse;Livertransplantation;Coldpreservationinjury;Hepaticarterialreconstruction;SurvivalrateUWHTK,[1],,,[24],[5,6],,(hepaticarterialreconstruction,HAR),C57BL/668,23~27gVectorLaboratories,Sigma,BoehringerMannheim,(DAB)VectorLaboratoriesBectonDicksonLeicaGZ6E(),Surgivet()5:(1)(coldpreservationtmie,CPT)1h,12;(2)CPT4h,12;(3)CPT8h,12;(4)CPT16h,16;(5)CPT16h+HAR,161:,,,,,,,,061mm,100U02ml,4∀UW2m,l,,4∀UW,,2:,,03ml[7],CPT16h+HARHAR,,:,,;,1362010513OrganTransplant,May2010,Vo.l1,No.3,,,;110,;,5,,,,,1:52:KaplanMeier5(10/)3:48h2,,14%,,,(HE)Suzuki,[8]11h(50mg/kg),,DAB,SPSS110#,,tKaplanMeier12dP005CPT16h+HAR,,(1)CPT1h4h8h1min,;CPT16h,,;CPT16h+HARHAR,,1CPT16h+HARCPT1h4h8h12d7/7,10/10,9/9CPT16h112d,36h,12d10%(1/10)CPT16h+HAR12d90%(9/10)12CPT16h,CPT16h+HAR(P001)48h,CPT1hCPT4h,,,;,CPT8h,;,CPT16h(90%36h,48h)CPT16h+HAR,,248h,CPT1h4h8hSuzuki(12#06)(14#05)(26#05)48h,CPT16hCPT16h+HARSuzuki(47#05)(31#07)1372010513OrganTransplant,May2010,Vo.l1,No.32:48h,CPT1h(2A)CPT8h(2B)CPT16h(2C)CPT16h+HAR(2D)(HE,∃100)48h,CPT1h(2E)CPT4h(2F)CPT8h(2G)CPT16h+HAR(2H)(,∃100),(P005),HARTian[9]HAR,HARSteger[10]Balb/c,CBA/Ca1382010513OrganTransplant,May2010,Vo.l1,No.3C57BL/10C57BL/6,C57BL/6,,HARCPT8h[3],HAR,C57BL/6HAR,,HAR,,8hCPT16h,,HAR16h,HAR[1113],(leukocyteentrapment)[1416],,,[1,17,18],T,,[1922],[2326],HARHAR,,,,HAR[1]SerracinoInglottF,HabibNA,MathieRTHepaticischemiareperfusioninjury[J]AmJSurg,2001,181(2):160166[2]DeboneraF,WangG,XieJ,etalSeverepreservationinjuryinducesIL6/STAT3activationwithlackofcellcycleprogressionafterpartiallivergrafttransplantation[J]AmJTransplant,2004,4(12):19641971[3]XieJF,WangG,DeboneraF,etalSelectiveneutralizationofthechemokineTCA3reducestheincreasedinjuryofpartialversuswholelivertransplantsinducedbycoldpreservation[J]Transplantation,2006,82(11):15011509[4],,30%[J],2009,24(5):383386[5]EricksonRPMousemodelsofhumangeneticdisease:whichmouseismorelikeaman?[J]Bioessays,1996,18(12):993998[6],,6[J],2008,23(7):507509[7],,[J],2006,29(5):371372[8]SuzukiS,ToledoPereyraLH,RodriguezFJ,etalNeutrophilinfiltrationasanimportantfactorinliverischemiaandreperfusioninjurymodulatingeffectsofFK506andcyclosporine[J]Transplantation,1993,55(6):12651272[9]TianY,RdigerHA,JochumW,etalComparisonofarterializedandnonarterializedorthotopiclivertransplantationinmice:prowessorrelevantmode?l[J]Transplantation,2002,74(9):12421246[10]StegerU,SawitzkiB,GasselAM,etalImpactofhepaticrearterializationonreperfusioninjuryandoutcomeaftermouselivertransplantation[J]Transplantation,2003,76(2):327332[11]ShenXD,GaoF,KeB,etalInflammatoryresponsesinanewmousemodelofprolongedhepaticcoldischemiafollowedbyarterializedorthotopiclivertransplantation[J]LiverTransp,l2005,11(10):12731281[12]ShenXD,KeB,ZhaiY,etalAbsenceoftolllikereceptor4(TLR4)si