Chymosin B from Aspergillus niger var. awamori con

CHYMOSINBfromASPERGILLUSNIGERvar.AWAMORIcontainingthePROCHYMOSINBGENEPreparedatthe53rdJECFA(1999)andpublishedinFNP52Add7(1999),supersedingtentativespecificationspreparedatthe37thJECFA(1990),publishedinFNP52(1992).ADINotspecifiedestablishedatthe37thJECFAin1990.SYNONYMSRennin,milk-clottingenzyme,chymosin,chymosinB,aspartylproteaseC.A.S.number85713-24-2SOURCESProducedextracellularlybythecontrolledfermentationofAspergillusnigervar.awamoricontainingthebovineprochymosinBgene.Thestrainisnon-pathogenicandnon-toxicogenic(forexample,NRRL3112).Afterinactivationoftheproductionorganism,thecellularmaterialisremovedbycentrifugationorfiltration.Theenzymeispurifiedbyextractionwithpolyethyleneglycol,followedbyclarificationwithactivatedcarbonandseparationfromthesolventbycationexchangechromatography.ActiveprinciplesChymosinSystematicnamesandnumbersNone(EC3.4.23.4)ReactionscatalyzedCleavesasinglebondinkappa-caseinDESCRIPTIONClear,colourlessorslightlycolouredaqueoussolutionscontainingtheactiveenzymeFUNCTIONALUSESEnzymepreparationUsedinclottingofmilkforcheeseproductionGENERALSPECIFICATIONSMustconformtotheGeneralSpecificationsforEnzymePreparationsusedinFoodProcessing(seeVolumeIntroduction)CHARACTERISTICSIDENTIFICATIONMilkclottingactivity(Vol.4)Thesampleshowsmilkclottingactivity