复方星夏汤对痰湿型多囊卵巢综合征-省略-瘤坏死因子-白细胞介素-6的影响-陈央娣

35720177CHINESEAＲCHIVESOFTＲADITIONALCHINESEMEDICINEVol．35No．7Jul．20171813DOI10．13193/j．issn．1673-7717．2017．07．048－α、－61221．3111062．310007PCOS－αTNF－α、－6IL－6PCOS。PCOS。23ICＲ724、、、－3518。、E2、T、LH、FSH、FPG、FINS、TNF－α、IL－6LH/FSH、HOMA－IＲ。、－35PCOSLH、LH/FSHFPG、FINS、HOMA－IＲTNF－αIL－6、HOMA－IＲ－35P＜0.01。TNF－α、IL－6IＲ。TNF－αIL－6Ｒ711．75A1673-7717201707-1813-052017－02－162014202013ZA095GZS20120231987－、、。1954－、。[e-mail]。EffectsofCompoundXinxiaDecoctiononTNF－αandIL－6inPhlegm－DampnessPolycysticOvarySyndromePCOSModelMiceCHENYangdi1FUPing2XINGJia21．GynecologyDepartmentHangzhouYuhangDistrictHospitalofTraditionalChineseMedicineHangzhou311106ZhejiangChina2．GynecologyofTCMHangzhouHospitalofTraditionalChineseMedicineHangzhou310007ZhejiangChinaAbstractObjectiveToobservetheeffectofCompoundXinxiaDecoctiononleveloftumornecrosisfactor－αTNF－αandinterleukin－6IL－6inphlegm－dampnesspolycysticovarysyndromePCOSmodelmiceandpreliminarilyexploretheactionmechanismofCompoundXinxiaDecoctiontreatingphlegm－dampnesspolycysticovarysyndromeP-COS．MethodsUsedehydroepiandr－osteronesulfatejointchainureawithcephalosporinsandhighfatandsugarfeedingmethodtoestablishphlegm－dampnesspolycysticovarysyndromeinmicemodel．Atotalof72femaleICＲmiceof23daysoldwereselectedandrandomlydividedinto4groupsnormalcontrolgroupmodelcontrolgroupCompoundXinxiaDecoctiongroupDiane－35group18miceineachgroup．ComparechangesofweightlevelsofE2TLHFSHFPGFINSTNF－αIL－6andtheLH/FSHratioandHOMA－IＲindexwerecalculated．Ovarieswereharvestedforpatholog-icalsection．Thechangesinhistomorphologywereobservedunderopticalmicroscope．ＲesultsCompoundXinxiaDecoctiongroupandDiane－35groupbothcanreduceweightsandthelevelsofLHtheLH/FSHratioFPGFINSHOMA－IＲin-dexandTNF－αkeeporrestorenormalovariantissuemorphologicalstructure．IntheaspectofreducingserumlevelsofIL－6andHOMA－IＲindexCompoundXinxiaDecoctiongroupwassignificantlysuperortoDiane－35groupP＜0.01．ConclusionCompoundXinxiaDecoctionmayimprovetheinsulinresistanceIＲthroughloweringtheserumlev-elsofTNF－αandIL－6andthendirectlyorindirectlyregulateendocrinehormoneandreproductiveaxistopromptma-turefollicledevelopmentandovulationultimatelyimprovereproductivecapacitywhichcouldbeonewayofCompoundXinxiaDecoctiontreatingphlegm－dampnessPCOS．KeywordsCompoundXinxiaDecoctionphlegm－dampnesspolycysticovariansyndromeTNF－αIL－6insulinresistance318135720177CHINESEAＲCHIVESOFTＲADITIONALCHINESEMEDICINEVol．35No．7Jul．20171814polycysticovariansyndromePCOS、、。1PCOSTNF－αIL－6PCOSTNF－α、IL－6PCOS。11．123ICＲ7213～18gSCXKTF-SX20080033。、10%、10%、5%、75%。1．2①12g10g15g15g15g15g15g15g15g20g5g9g。0．806g/mL4℃。②－35ScheringGmbH＆Co．ProduktionsKG2mg0．035mgJ201000030．01mg/mL。③DHEA99%。④STZsigma≥98%0．1mmol/LSTZ30min。1．3232d。72、、、－35418。、、－35。1．41．4．12、、－350．9mg/10gDHEA0．1mL34d46。468h8∶00～16∶00、、－350．1mL/10gSTZ1。1．4．20．3mL/10g·d34d46－35－350．3mL/10g·d34d46、。1．4．334d12h。1．51．5．1。1．5．212hFPG。1．5．3－αTNF－α、－6IL－6、E2、T、LH、FSH、FINS。HOMA－IＲ3HOMA－IＲ=mmol/L×mIU/L/22．5。1．5．4。1．6SPSS17．0±珋x±sone－sampleKolmogorov－SmimovtestLevene’st。P＜0.05。22．1、－35、P＞0.05。1。1珋x±snggg1715．522±1．165▲27．122±1．131#11．600±2．559#1415．278±0．94332．285±0．84317．007±1．7391515．144±1．355▲29．504±1．146●14．360±1．891●－351515．653±1．355▲28．673±0．964#13．020±2．177#●P＜0.05#P＜0.01▲P＞0.05。2．2FINS、FPGHOMA－IＲP＜0.01。－35FINS、FPG、HOMA－IＲP＜0.01HOMA－IＲ－35P＜0.05。2。2FINS、FPG、HOMA－IＲn=9珋x±smIU/Lmmol/LHOMA－IＲ10．163±0．702●5．944±0．889●2．674±0．372●13．756±0．5629．567±0．6525．846±0．4228．601±0．503●＊＊6．522±0．624●＊＊2．498±0．325●△△－359．418±1．632●7．278±0．498●3．075±0．896●●P＜0.01－35△△P＜0.05＊＊P＞0.05。2．3T、LHLH/FSH418135720177CHINESEAＲCHIVESOFTＲADITIONALCHINESEMEDICINEVol．35No．7Jul．201718153E2、T、LH、FSH、LH/FSHn=9珋x±sng/Lnmol/Lng/LIU/LLHFSH48．557±11．096▲135．382±1．649●1405．441±83．389●7．466±0．316#5．636±0．668●51．001±3．911140．182±5．2911761．962±54．7128．154±0．1296．487±0．57443．272±4．593#136．957±3．244▲1436．653±34．203●7．815±0．070▲5．521±0．456●－3533．726±3．028●138．899±3．298▲1112．255±31．618●6．243±0．086●5．346±0．421●●P＜0.01#P＜0.05▲P＞0.05。P＜0.01FSHP＜0.05E2P＞0.05。E2P＜0.05－35E2P＜0.01。TP＞0.05－35TP＞0.05。－35LH、LH/FSHP＜0.01。FSHP＞0.05。3。2．4－α、－6TNF－αP＜0.01IL－6P＜0.05。－35TNF－αP＜0.01IL－6P＜0.01。－35IL－6P＜0.01TNF－αP＞0.05。－35LHP＞0.05。4、1。2．5ⅩⅤ2。8～9、、。2～31、、。、－358～9、。－35。－35。4TNF－α、IL－6n=9珋x±s－αng/L－6pg/mL389．525±37．105●23．735±9．010#431．736±42．61830．463±3．630371．627±19．128●△△12．735±2．566●＊＊－35389．300±13．520●25．694±2．887▲●P＜0.01#P＜0.05▲P＞0.05－35△△P＞0.05＊＊P＜0.01。3PCOSIＲ。TNF－α、IL－6PCOS4PCOSIＲ5。TNF－α、、、。Tar-kunI6PCOSTNF－αPCOS●P＜0.01#P＜0.05▲P＞0.05－35△△P＞0.05＊＊P＜0.01。1TNF－α、IL－6珋x±s518135720177CHINESEAＲCHIVESOFTＲADITIONALCHINESEMEDICINEVol．35No．7Jul．20171816TNF－αPCOS。2ICＲPCOSPCOSTNF－αP＜0.01PCOS。IL－6、、。IL－67－9IＲIL－6IＲ10－11。IL－6IＲan-drogenreceptorAＲMAPK12PCOSIL－6IL－6TBβIＲ13。IL－614。PCOSIL－6P＜0.01FINS、FPGHOMA－IＲP＜0.01IL－6T、LHLH/FSHP＜0.01IL－6PCOS。PCOS“”、“”、“”。PCOS。。40PCOSPCOS。、、“”、“”。。“”“”。PCOS。HEPCOS、LH、FINS、FPG、TNF－α、IL－6LH/FSH、HOMA－IＲTIL－6HOMA－IＲ－35。PCOS。TNF－α、IL－6IＲPCOS。1KellyCCLyallHPetrieJＲetal．Lowgradechronicinflamma-tioninwomenwithpolycysticovariansyndromeJ．JClinEndo-crinolMetab20018662453－2455．2．J．201533102367－2369．3LegroＲSCastracaneVDKauff