DB61∕T 408-2007 牛奶中D一乳酸 L乳酸的测定-酶法

DB61if~JFiftDB61/T408-~20072007-09-03~~2007-09-03~~!IDB61IT408-2007I!J*:fJFffEr:p~~ft:i'RAjJ~*411Ilft:i'R.11*~ffE~r:p$A~~~OOM@iliA~~~~&~.ilifr~p.*~ffE®~.~:r:p$A~~~OO.~WA~~~~&~.*~ffE~.A:~*~,~m*,*~ili.~a•.*;fJFffE~tti'xtt;ffl•1If\DB61tr408-2007;;js:*ffiftE1mT~1t;:~yl~JE4JgJ9D-~Lft/L~Lmil¥J~:wI¥r7J¥t;:o'*1~~ftE13:J+]T4W)~D-~L~/L~LftI¥JWl~JE02;m5iHiijIm3i:f!fr')lUX1'1:9B\J~~JEli1;;js:¥j\1lI¥Ji]IJ+jTIDJJX;))**~fitEJ~~ofL1id1E3,1tIjtEJijIJ+jX1'1:,:It[)}fiJFiFn{TA1.~.(~~Wm~~~3)~.U~~~~J+]T;;js:~m,~TID,.~••;;js:~ftE~~~~tEJ~~~~liki!fi5J1!fJij:l!%J;:x1'Hn.ii:~~;;js:o}1;li!:;~I£E3Jt~I¥J~IJ+]X14,;1=tiiWr~*13:J·tlr;;js:*ffiftE0EN-ISO80693[)J.ltWJ9?¥L~Ec~Lft~*]ttA1~~~EN12631**:fIIJfi~dt9D-~Lmi;fI]L~L~(~)I¥J~~~JEDIN103354WJ:f114WJ1ttlM9D-/L-~LM~~1~JED-LDHL-LDHNADNADHGPTUACD-~LftJl£tI.!WL-~L~Jl£~UWM~£IJ:,~n~pt--=~1rMj3-1W~-~n~~--~ft1TM~:fr~~p;jill0~$'&H~~Illijnfr1.i00~ffi1fT.,JJX;111rno1I¥J4ioJ.at0~,&:Ytm{Jl;mN!tr~i:&,Crt41*11..Ii1':fD-~L~Jl£tI.!W(D--LDH)f¥tfA1~1lF,D-!JiLlW1JJtjWMH~ID'ftPj!pt~~tr~(NAD)¥t1tJJX;p;Jill~~(pyruvate),L-!¥L~fffi~tfL-!JiL~Jl£ttl,fCL-LDH)fffta1;;t~~flll¥t1toJiJIV.5:tJ,):[H.OII(0D-Lactate+NAD~pyruvate+NADH+II(2)L-Lactate+NADLUlll~pyruvaet+NADH+H&JiY.1¥J'¥1iJJt(lkT!¥LMI¥J1$toJEluL&!tIJ.Hililjlp,ftL-i't~1W(L-·glutamatc)ff{±R1~{'I:!,.f&:j§-tk~p;Jfj]~~$i{ij~~CGPT)H1HtR~j:q1ijl~IW~(pyruvae),&J\Y.'¥f~jn;fIjTjAJJiJW~(pyruvae);fllNADIII¥.J1:.JJX;,JiJ.i\f.:r\JJ;DB61/T408-2007GPT(3) Pyruvate+L--glutarnate~L-alanine+2~oxoglutarate1?J.1:B(@.p%Jix;i¥JNADHD-Lactate.L-Lactatei¥JW7t!ijIJfftE1.t~itit)t~o1:fiX:f¥1NADHi¥Jn)l7tJ.l1IfilJ:{f340nmr~IJ:m:o5.1)(J{itJ:B;t5.2NAD5.3GPT5.4D~LDH5.5L~LDH5.6HCl5.7NaOH5.8KOH_fl}.J5.9'li~:K5.10illPJ1!tJt]6.1f$~lHE:(5mL.1mL50~200l.lL1O~50JlL)6.2btellIl:7tf5::1.0cm6.3~3J~5t7t7tJ.lit,TlJ3k:@(K,fr:HOnm~W06.41JT~1~~~,JLH10Jlm06.56.6'tBjll1ilkm@6.771~nJE~iia~6.8~{l'fJl6.9~~~,m~,~~AA.B.~~ft~~,~4,.~W~7.1lIt1fIJj!jl*U7.1.1~1tj[H.t~;~;~flFlfX4.75gXXit~J;t,f110.88R;f¥1L-fiJ:lW.ML,110A60mUIH1~*,J£7tm~lt-Jfl,)fj2mol/LNaOHV~pH¥1O.0;7.1.2 NADI1i;~1!t}+lB1,ImLJI~7JJ.f¥M35mg=f*)}liPJJNADT1f¥~o7.1.3GPT~~¥Hif~}fjfit,}+lO.7mLm:,$)Km:~lf.ll00Ui¥JGPT=f;fJJlmJJGPT;~,J1~o7.1.4 D-LDH;gHi1~JtJfI't.JtJO.7mL1M:t4)KmMA~13800[JIf'.lD-LDI-Ic7.1.5 L-LDHigHif!t,LB111,,LBO.7mL:lli:~*mffFL.k,j13800Ui¥JD-LDHo7.1.6He):2moIlL7.1.7NaOH:2moi/L2DB61n'408-20077.1.8KOH:2mollL1.2lit~ffiIJ1i!fX.2mL(g)4tJj,noA98mUlU~J./,mr~l1ifE~th~/~H~lOmin,1t5t1~~,ii~,7J!~J.jr-:ftEtWlIJo1.3#tWIlj(jJt~1ilHT)~ 1.3.1i9:~s~-t!m:!fX.bt~llJl,f-&(xnl~A1.OOOmLX){i=t1!B:tgl{lP~,O.200mLNADIf'f~,0.020mLGPT;t;k~,1.000mL ~J./,~itJ!i5minJ§-@;!fX.D&JtlJila(AI),noAO.02mLD-LDHm~,M'nBir&&jG1£(*~'130min),~!fX.ll&JtfJtillCAz),f!}tmAO.02mLL-LDHm~,tJ¥~it&)\IljG1£(jdJ30min),.~!fX.f1&JtlJi{a(A3),~SD&JtJ.im.&.=CAeAJ)'~~J;.j,~C7.3.2'l'+1::n::p!fX.bt~lIll.,f-&ixnoA1.OOOmL)(J.i:t1!B:t~{lP~,O.200mLNAD.I~ff~,O.020mLGPT,t;k7¥~,0.100mL1J.\t¥?~,4~/j(O.900mLlft-frJl5minFn@;!fX.ll,&JtJ!£lHCAl),tlnAO.02mLD-LDHm~,t;'n~it&&7iifJ:X;(:k~J30min),-@;!fX.D&jt[\[{tl:(A2),MnoA0.02mLL--IDHm~,*¥~ft&&jGJiX(:A~J30min),~!fX.!1&Jt&lli(A3),H®D&Jt[\[fJl#=(AI)l'Ia;~1#l;9!ljl~~5t)JIJ~~~1E3::8L.jf4':*I¥JD,&jt[\[{Jlti:CA2-AIJ,JiJt+MD&.ltJJt1Jl#~-:t3::An&jtJl{1l~WJI;1~~U0A(1):f:,AIJ-'il,~=[A2--AIJIH-[A,,-A:]UI..·······,,·,,..···•..·...............(1):5J\.£IJ~1tl1E:i:AJ'JH*rr~Il&.ltlJim.&.[A,-AJ,mth~INjtl!H§:ft~-:t~Ar&jtJllLjl1rilJ1;1f~~IJ0A(2):f:,AL-~t!W=[A,-A2JII{'-[A,-A,]','1'1'.....................................··(2)8it.itHI~0J:\.(3)itm:tR&:!------XLjAXf·....········....·..............··..········..··(3)txdxV::vx1000NADT1f~GPT:~Hj£~t¥~~m11?i1£~J./1.000mL0.200mL0.02mL1.000mL1.000mLO.200mL0.02rnLO.100mLO.900mL2.240X90.1CXLj&~XftX1.00X O.100X10002.018,,3DB61fT408-2007L-~L~*ftitrt:0j\;2.260X90.1CXL:1AI.1UItXf£X1.00XO.100X10002.036CV-MIt(g/U¥rfldR1ff)--~----X100(g/100g)..............................(4)C;IUIf(g/Uflldffilf)G.-liAr=-----~--X100(g/100g)....•··....·....·..··..·..·..··..(5)c~~WluW,'9fI¥J#{!.!r(giL)MW~~l:9lljWJPtI¥JffjJ(!ViiiL~L~(~jJ\mw90.19!mol)Illi:~Ul~LU:(~jJ(!Viii89.1g/mol)VIct~J]l4'~Jlljlit¥fH~I¥J/\f;,1*fJ(,~1f7:~mL~~~*~I¥J~£m.I¥J~1*~,.~~~ff¥nhm.I1'J~iff:itt~d~~5J]lI¥JJtW,~{Ir.~emNADPHI¥JWl7t~:rz:ft340run=6.3Lmmor-1em~IL1Ar~~Jt&fft#9.1D-¥LIUL¥L~MitS:?:;~Hi£-1-*ff-/l!f.JD-¥L~IL¥L~*~ifi9.2MitS:?:!f.Jmm9.2.1;~1JnMitS:?:j~;fiflj&mf**Jtl09.2.2IiFfi1H~i1!fT~,;,&mf¥~M~'tRt&JiY.tR-*t,AzinB3;W,tuO.050mll¥J!Vir£Rt11QfIJfft~JlU¥l:B-frm~,&JlY.FnW:}f,(lOmin),••~@I¥J~j\;(Ar~)#rt:#{&~,JiY.~~~&~m1*~o~~Jt!.!rffi~o~~~.~~Jt&ffi~11u.9.2.3~f;F4DB611T408-2007:tJnA~t~;fft:pl¥Jm~AffMlf¥1:lrm-nn+;]\-~-xxi=t~n;!dll{tP~NAD.f1'f.~GPT;~¥¥~fffi1r.tm~D-~L~JW:t:lm~EixL1L~JW:t:lm~m~;J1.000mL0.200mLO.020mL-1.000mL1.000mLO.200mLO.020mLO.lOOmL-O.900mL1.OOOmL0.200mLO.020mL-O.100mLO.900mL1.OOOmL0.200mL0.020mLO.050mLO.050mL0.900mL1liil5iH-rrJR~:tttrl&.jt;/J[11I[AI],~JRt7.3;ff.z~~2t~~til;ff.z~~la~r¥'----­m~~1ta)X100(%)..............................'''(6)5DB61ff408~20072,btjflj.p,!IIG1tIJYJI¥J~{tjl~,~JiIj~T~}~»Y.ff.r2·C~8·Co~1tuf.iX;1¥JNAD]:ft~fr2·C~8·CWJ~SE=jii],I£{f-15·C~-25·C~f4t-'iiJ1*fJW'i1'-fja~1tuf.iX;FlJD-MDH.L